GRF9_ARATH
ID GRF9_ARATH Reviewed; 431 AA.
AC Q8S9M3; F4IG65; O22125; Q8S8T9;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Growth-regulating factor 9;
DE Short=AtGRF9;
DE AltName: Full=Transcription activator GRF9;
GN Name=GRF9; OrderedLocusNames=At2g45480; ORFNames=F17K2.27, F4L23.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12974814; DOI=10.1046/j.1365-313x.2003.01862.x;
RA Kim J.H., Choi D., Kende H.;
RT "The AtGRF family of putative transcription factors is involved in leaf and
RT cotyledon growth in Arabidopsis.";
RL Plant J. 36:94-104(2003).
RN [6]
RP INDUCTION.
RX PubMed=15200956; DOI=10.1016/j.molcel.2004.05.027;
RA Jones-Rhoades M.W., Bartel D.P.;
RT "Computational identification of plant microRNAs and their targets,
RT including a stress-induced miRNA.";
RL Mol. Cell 14:787-799(2004).
RN [7]
RP TISSUE SPECIFICITY, AND INTERACTION WITH GIF1.
RX PubMed=15960617; DOI=10.1111/j.1365-313x.2005.02429.x;
RA Horiguchi G., Kim G.T., Tsukaya H.;
RT "The transcription factor AtGRF5 and the transcription coactivator AN3
RT regulate cell proliferation in leaf primordia of Arabidopsis thaliana.";
RL Plant J. 43:68-78(2005).
RN [8]
RP INDUCTION.
RX PubMed=19453503; DOI=10.1111/j.1399-3054.2009.01229.x;
RA Liu D., Song Y., Chen Z., Yu D.;
RT "Ectopic expression of miR396 suppresses GRF target gene expression and
RT alters leaf growth in Arabidopsis.";
RL Physiol. Plantarum 136:223-236(2009).
RN [9]
RP DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=20023165; DOI=10.1242/dev.043067;
RA Rodriguez R.E., Mecchia M.A., Debernardi J.M., Schommer C., Weigel D.,
RA Palatnik J.F.;
RT "Control of cell proliferation in Arabidopsis thaliana by microRNA
RT miR396.";
RL Development 137:103-112(2010).
RN [10]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=21036927; DOI=10.1093/jxb/erq307;
RA Wang L., Gu X., Xu D., Wang W., Wang H., Zeng M., Chang Z., Huang H.,
RA Cui X.;
RT "miR396-targeted AtGRF transcription factors are required for coordination
RT of cell division and differentiation during leaf development in
RT Arabidopsis.";
RL J. Exp. Bot. 62:761-773(2011).
CC -!- FUNCTION: Transcription activator that plays a role in the regulation
CC of cell expansion in leaf and cotyledons tissues. Component of a
CC network formed by miR396, the GRFs and their interacting factors (GIFs)
CC acting in the regulation of meristem function, at least partially
CC through the control of cell proliferation.
CC {ECO:0000269|PubMed:21036927}.
CC -!- SUBUNIT: Interacts with GIF1. {ECO:0000269|PubMed:15960617}.
CC -!- INTERACTION:
CC Q8S9M3; Q8L8A5: GIF1; NbExp=4; IntAct=EBI-1396638, EBI-1396623;
CC Q8S9M3; Q9MAL9: GIF2; NbExp=3; IntAct=EBI-1396638, EBI-1396863;
CC Q8S9M3; Q93VH6: GIF3; NbExp=3; IntAct=EBI-1396638, EBI-15194507;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU01002}.
CC -!- TISSUE SPECIFICITY: Detected in the shoot apical meristem (SAM) and in
CC young leaf primordium. {ECO:0000269|PubMed:15960617,
CC ECO:0000269|PubMed:21036927}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the early stages of leaf
CC development and expression decreases with the maturation of the leaf.
CC {ECO:0000269|PubMed:20023165}.
CC -!- INDUCTION: microRNA 396 (miR396a or miR396b) negatively regulates
CC growth-regulating factors (GRF1-4 and GRF7-9).
CC {ECO:0000269|PubMed:15200956, ECO:0000269|PubMed:19453503,
CC ECO:0000269|PubMed:20023165}.
CC -!- DOMAIN: The QLQ domain and WRC domain may be involved in protein-
CC protein interaction and DNA-binding, respectively.
CC -!- SIMILARITY: Belongs to the GRF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM14831.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AEC10560.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC002387; AAM14831.1; ALT_INIT; Genomic_DNA.
DR EMBL; AC003680; AAF18607.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC10560.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002685; ANM61817.1; -; Genomic_DNA.
DR EMBL; AY074647; AAL69463.1; -; mRNA.
DR EMBL; AB493593; BAH30431.1; -; mRNA.
DR PIR; A84891; A84891.
DR PIR; T00861; T00861.
DR RefSeq; NP_001324014.1; NM_001337134.1.
DR RefSeq; NP_850438.2; NM_180107.3.
DR AlphaFoldDB; Q8S9M3; -.
DR BioGRID; 4492; 5.
DR IntAct; Q8S9M3; 5.
DR STRING; 3702.AT2G45480.1; -.
DR PaxDb; Q8S9M3; -.
DR PRIDE; Q8S9M3; -.
DR ProteomicsDB; 247289; -.
DR EnsemblPlants; AT2G45480.3; AT2G45480.3; AT2G45480.
DR GeneID; 819156; -.
DR Gramene; AT2G45480.3; AT2G45480.3; AT2G45480.
DR KEGG; ath:AT2G45480; -.
DR Araport; AT2G45480; -.
DR TAIR; locus:2043595; AT2G45480.
DR eggNOG; ENOG502RD11; Eukaryota.
DR HOGENOM; CLU_038997_0_0_1; -.
DR InParanoid; Q8S9M3; -.
DR OrthoDB; 823826at2759; -.
DR PhylomeDB; Q8S9M3; -.
DR PRO; PR:Q8S9M3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8S9M3; baseline and differential.
DR GO; GO:0005634; C:nucleus; ISS:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0048366; P:leaf development; TAS:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR014978; Gln-Leu-Gln_QLQ.
DR InterPro; IPR031137; GRF.
DR InterPro; IPR014977; WRC_dom.
DR PANTHER; PTHR31602; PTHR31602; 2.
DR Pfam; PF08880; QLQ; 1.
DR Pfam; PF08879; WRC; 2.
DR SMART; SM00951; QLQ; 1.
DR PROSITE; PS51666; QLQ; 1.
DR PROSITE; PS51667; WRC; 2.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..431
FT /note="Growth-regulating factor 9"
FT /id="PRO_0000419300"
FT DOMAIN 24..59
FT /note="QLQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01001"
FT DOMAIN 89..133
FT /note="WRC 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT DOMAIN 307..351
FT /note="WRC 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT MOTIF 94..104
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT MOTIF 122..129
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT MOTIF 312..322
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT MOTIF 340..345
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
SQ SEQUENCE 431 AA; 48867 MW; 28756B46F614A288 CRC64;
MKMQSPKMEQ EEVEEERMRN KWPWMKAAQL MEFRMQALVY RYIEAGLRVP HHLVVPIWNS
LALSSSSNYN YHSSSLLSNK GVTHIDTLET EPTRCRRTDG KKWRCSNTVL LFEKYCERHM
HRGRKRSRKL VESSSEVASS STKYDNTYGL DRYNESQSHL HGTISGSSNA QVVTIASLPS
ARSCENVIRP SLVISEFTNK SVSHGRKNME MSYDDFINEK EASMCVGVVP LQGDESKPSV
QKFFPEVSDK CLEAAKFSSN RKNDIIARSR EWKNMNVNGG LFHGIHFSPD TVLQERGCFR
LQGVETDNEP GRCRRTDGKK WRCSKDVLSG QKYCDKHMHR GMKKKHPVDT TNSHENAGFS
PLTVETAVRS VVPCKDGDDQ KHSVSVMGIT LPRVSDEKST SSCSTDTTIT DTALRGEDDD
EEYLSLFSPG V
