ID   GRFIN_GRISQ             Reviewed;         121 AA.
AC   P84801;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   02-JUN-2021, entry version 46.
DE   RecName: Full=Griffithsin;
DE            Short=GRFT;
OS   Griffithsia sp. (strain Q66D336) (Red alga).
OC   Eukaryota; Rhodophyta; Florideophyceae; Rhodymeniophycidae; Ceramiales;
OC   Ceramiaceae; Griffithsia; unclassified Griffithsia.
OX   NCBI_TaxID=373036;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RX   PubMed=15613479; DOI=10.1074/jbc.m411122200;
RA   Mori T., O'Keefe B.R., Sowder R.C. II, Bringans S., Gardella R., Berg S.,
RA   Cochran P., Turpin J.A., Buckheit R.W. Jr., McMahon J.B., Boyd M.R.;
RT   "Isolation and characterization of griffithsin, a novel HIV-inactivating
RT   protein, from the red alga Griffithsia sp.";
RL   J. Biol. Chem. 280:9345-9353(2005).
CC   -!- FUNCTION: Mixed specificity lectin with anti-HIV activity. Binds to HIV
CC       envelope glycoproteins, including exterior membrane glycoprotein gp120,
CC       and inhibits viral entry into cells. Binding to gp120 is dependent on
CC       gp120 being glycosylated, and is inhibited by mannose, glucose and N-
CC       acetylglucosamine. {ECO:0000269|PubMed:15613479}.
CC   -!- INTERACTION:
CC       P84801; P84801: -; NbExp=3; IntAct=EBI-8453570, EBI-8453570;
CC       P84801; Q75760: env; Xeno; NbExp=2; IntAct=EBI-8453570, EBI-8453491;
CC   -!- MASS SPECTROMETRY: Mass=12770.05; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:15613479};
CC   -!- SIMILARITY: Belongs to the jacalin lectin family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01088, ECO:0000305}.
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DR   PDB; 2GTY; X-ray; 1.30 A; A/B=1-121.
DR   PDB; 2GUC; X-ray; 1.79 A; A/B=1-121.
DR   PDB; 2GUD; X-ray; 0.94 A; A/B=1-121.
DR   PDB; 2GUE; X-ray; 2.02 A; A/B=1-121.
DR   PDB; 2GUX; X-ray; 2.00 A; A=1-121.
DR   PDB; 2HYQ; X-ray; 2.00 A; A/B=1-121.
DR   PDB; 2HYR; X-ray; 1.51 A; A/B=1-121.
DR   PDB; 2NU5; X-ray; 1.56 A; A/B=1-121.
DR   PDB; 2NUO; X-ray; 1.50 A; A/B=1-121.
DR   PDB; 3LKY; X-ray; 1.11 A; A=1-121.
DR   PDB; 3LL0; X-ray; 1.70 A; A=1-121.
DR   PDB; 3LL1; X-ray; 0.97 A; A=1-119.
DR   PDB; 3LL2; X-ray; 0.97 A; A=1-121.
DR   PDBsum; 2GTY; -.
DR   PDBsum; 2GUC; -.
DR   PDBsum; 2GUD; -.
DR   PDBsum; 2GUE; -.
DR   PDBsum; 2GUX; -.
DR   PDBsum; 2HYQ; -.
DR   PDBsum; 2HYR; -.
DR   PDBsum; 2NU5; -.
DR   PDBsum; 2NUO; -.
DR   PDBsum; 3LKY; -.
DR   PDBsum; 3LL0; -.
DR   PDBsum; 3LL1; -.
DR   PDBsum; 3LL2; -.
DR   BMRB; P84801; -.
DR   SMR; P84801; -.
DR   DIP; DIP-29130N; -.
DR   IntAct; P84801; 1.
DR   MINT; P84801; -.
DR   UniLectin; P84801; -.
DR   EvolutionaryTrace; P84801; -.
DR   GO; GO:0030246; F:carbohydrate binding; IDA:UniProtKB.
DR   GO; GO:0005536; F:glucose binding; IDA:UniProtKB.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0005537; F:mannose binding; IDA:UniProtKB.
DR   GO; GO:0046871; F:N-acetylgalactosamine binding; IDA:UniProtKB.
DR   Gene3D; 2.100.10.30; -; 1.
DR   InterPro; IPR001229; Jacalin-like_lectin_dom.
DR   InterPro; IPR036404; Jacalin-like_lectin_dom_sf.
DR   Pfam; PF01419; Jacalin; 1.
DR   SMART; SM00915; Jacalin; 1.
DR   SUPFAM; SSF51101; SSF51101; 1.
DR   PROSITE; PS51752; JACALIN_LECTIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Lectin; Mannose-binding.
FT   CHAIN           1..121
FT                   /note="Griffithsin"
FT                   /id="PRO_0000228815"
FT   DOMAIN          1..120
FT                   /note="Jacalin-type lectin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01088"
FT   STRAND          2..8
FT                   /evidence="ECO:0007829|PDB:2GUD"
FT   STRAND          12..15
FT                   /evidence="ECO:0007829|PDB:2GUD"
FT   STRAND          20..34
FT                   /evidence="ECO:0007829|PDB:2GUD"
FT   STRAND          37..40
FT                   /evidence="ECO:0007829|PDB:2GUD"
FT   STRAND          44..46
FT                   /evidence="ECO:0007829|PDB:2GUD"
FT   STRAND          58..76
FT                   /evidence="ECO:0007829|PDB:2GUD"
FT   STRAND          81..86
FT                   /evidence="ECO:0007829|PDB:2GUD"
FT   STRAND          90..120
FT                   /evidence="ECO:0007829|PDB:2GUD"
SQ   SEQUENCE   121 AA;  12731 MW;  F89843AF6DE5049D CRC64;
     SLTHRKFGGS GGSPFSGLSS IAVRSGSYLD XIIIDGVHHG GSGGNLSPTF TFGSGEYISN
     MTIRSGDYID NISFETNMGR RFGPYGGSGG SANTLSNVKV IQINGSAGDY LDSLDIYYEQ
     Y