ID   GRH1_CAEEL              Reviewed;         563 AA.
AC   G5EDF0;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Grainyhead-like protein 1 homolog {ECO:0000312|WormBase:Y48G8AR.1a};
GN   Name=grh-1 {ECO:0000312|WormBase:Y48G8AR.1a};
GN   ORFNames=Y48G8AR.1 {ECO:0000312|WormBase:Y48G8AR.1a};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|EMBL:AAP78770.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=12888489; DOI=10.1093/nar/gkg644;
RA   Venkatesan K., McManus H.R., Mello C.C., Smith T.F., Hansen U.;
RT   "Functional conservation between members of an ancient duplicated
RT   transcription factor family, LSF/Grainyhead.";
RL   Nucleic Acids Res. 31:4304-4316(2003).
RN   [2] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Probable transcription factor that binds a motif with the
CC       core sequence 5'-C[ACT][TG]G-3'. {ECO:0000269|PubMed:12888489}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in embryos, larvae and adults.
CC       {ECO:0000269|PubMed:12888489}.
CC   -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in defective
CC       cuticle development (PubMed:12888489). RNAi-mediated knockdown in L4
CC       stage larvae has no effect, but upon reaching adulthood, over 95% of
CC       the embryos produced by these animals arrest and fail to hatch
CC       (PubMed:12888489). Development arrests at the three-fold stage, showing
CC       abnormal constrictions or puckering in the external cuticle and
CC       approximately 8% of embryos exhibit ruptures in their cuticles
CC       resulting in the extrusion of cells (PubMed:12888489).
CC       {ECO:0000269|PubMed:12888489}.
CC   -!- SIMILARITY: Belongs to the grh/CP2 family. Grainyhead subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AY323527; AAP78770.1; -; mRNA.
DR   EMBL; BX284601; CCD72954.1; -; Genomic_DNA.
DR   RefSeq; NP_490837.2; NM_058436.4.
DR   AlphaFoldDB; G5EDF0; -.
DR   SMR; G5EDF0; -.
DR   STRING; 6239.Y48G8AR.1; -.
DR   EPD; G5EDF0; -.
DR   PaxDb; G5EDF0; -.
DR   PeptideAtlas; G5EDF0; -.
DR   EnsemblMetazoa; Y48G8AR.1a.1; Y48G8AR.1a.1; WBGene00001707.
DR   GeneID; 190048; -.
DR   CTD; 190048; -.
DR   WormBase; Y48G8AR.1a; CE34614; WBGene00001707; grh-1.
DR   eggNOG; KOG4091; Eukaryota.
DR   HOGENOM; CLU_005625_3_1_1; -.
DR   InParanoid; G5EDF0; -.
DR   OMA; LMVVFRE; -.
DR   PhylomeDB; G5EDF0; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   ExpressionAtlas; G5EDF0; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR   GO; GO:0007568; P:aging; IMP:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR007604; CP2.
DR   Pfam; PF04516; CP2; 1.
DR   PROSITE; PS51968; GRH_CP2_DB; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..563
FT                   /note="Grainyhead-like protein 1 homolog"
FT                   /id="PRO_0000451597"
FT   DOMAIN          194..428
FT                   /note="Grh/CP2 DB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01313"
FT   REGION          326..335
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NZI5"
FT   REGION          372..375
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NZI5"
FT   REGION          377..405
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   563 AA;  64052 MW;  5D4F29CEE04CE769 CRC64;
     MSFQLDQSTS VIKTAPPGRL EFKNEPIDIA SSNIQDLAKM QTLDGYYQQT SLQLPQSQQY
     HRMYENVYNN QQILYAQTQN FQYPSTLLTN GSLAQTEWRG QQMDTSAYSN TGYSRSSTSQ
     QPQYTQQVTE NERIVYKPVD MPSPVDSGIG GDISILNPKE EFFTSADGGS MLERSSERTL
     SHRDSPLVIP KLYNNLGFQY VLEAPISTSV RRDDDRMTYV NKGQFYTVSL EYTPDLNKCL
     KSQTVKSQLM VVFREDKTYE EEIKTWQSWH ARQHVSKQRI LEIDSKNSSG MIGQIEEIGN
     NAVQFYWNPS DPSGVRISIA VQCLSTDFST QKGVKGLPLH VQIDTYDGEN DKVPFHRGYC
     QIKVFCDKGA ERKLRDEDKR AQKRKVQEYT AGALPGGRKK SDGEYHDQCE RSEFYHMREL
     DKPAALFIAP EEFEPRYVDS TSLSFDMSEI EPIPTKRPRT SERIMLYVRK RDEQIYQPLH
     VVPASLSGLA LAIANKFGAD PDKMSGVYKR CAKGITVKVD DEMLRLYCNE DTFIIDVEHA
     TDGSTAATLI EVAPTNPNSY SNS