GRHL2_XENTR
ID GRHL2_XENTR Reviewed; 619 AA.
AC Q5M7R9; Q28D77;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Grainyhead-like protein 2 homolog;
DE AltName: Full=Transcription factor CP2-like 3;
GN Name=grhl2; Synonyms=tfcp2l3; ORFNames=TGas068d14.1;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Gastrula;
RG Sanger Xenopus tropicalis EST/cDNA project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Neurula;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcription factor playing an important role in primary
CC neurulation and in epithelial development. Binds directly to the
CC consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and
CC repressor on distinct target genes. {ECO:0000250|UniProtKB:Q6ISB3,
CC ECO:0000250|UniProtKB:Q8K5C0}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q6ISB3}. Membrane
CC {ECO:0000250|UniProtKB:Q6ISB3}. Note=detected at cell-cell contact
CC areas. {ECO:0000250|UniProtKB:Q6ISB3}.
CC -!- MISCELLANEOUS: GRHL genes (GRHL1, GRHL2 and GRHL3) show a paradoxal
CC lack of redundancy despite their extensive sequence identity in the
CC DNA-binding and protein dimerization domains and the fact that the core
CC consensus DNA binding sites are identical. They have related but
CC remarkably different functions during embryogenesis because of their
CC differential spatiotemporal expression patterns during development.
CC {ECO:0000250|UniProtKB:Q8K5C0}.
CC -!- SIMILARITY: Belongs to the grh/CP2 family. Grainyhead subfamily.
CC {ECO:0000305}.
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DR EMBL; CR855659; CAJ83880.1; -; mRNA.
DR EMBL; BC088489; AAH88489.1; -; mRNA.
DR RefSeq; NP_001011338.1; NM_001011338.1.
DR AlphaFoldDB; Q5M7R9; -.
DR SMR; Q5M7R9; -.
DR PaxDb; Q5M7R9; -.
DR PRIDE; Q5M7R9; -.
DR DNASU; 496802; -.
DR Ensembl; ENSXETT00000014534; ENSXETP00000014534; ENSXETG00000006632.
DR GeneID; 496802; -.
DR KEGG; xtr:496802; -.
DR CTD; 79977; -.
DR Xenbase; XB-GENE-485995; grhl2.
DR eggNOG; KOG4091; Eukaryota.
DR HOGENOM; CLU_021156_1_1_1; -.
DR InParanoid; Q5M7R9; -.
DR OrthoDB; 286319at2759; -.
DR Proteomes; UP000008143; Chromosome 6.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000006632; Expressed in mesonephros and 7 other tissues.
DR ExpressionAtlas; Q5M7R9; baseline and differential.
DR GO; GO:0005911; C:cell-cell junction; ISS:UniProtKB.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0001161; F:intronic transcription regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0070830; P:bicellular tight junction assembly; ISS:UniProtKB.
DR GO; GO:0007420; P:brain development; IBA:GO_Central.
DR GO; GO:0007155; P:cell adhesion; ISS:UniProtKB.
DR GO; GO:0034329; P:cell junction assembly; ISS:UniProtKB.
DR GO; GO:0003382; P:epithelial cell morphogenesis; ISS:UniProtKB.
DR GO; GO:0001843; P:neural tube closure; ISS:UniProtKB.
DR GO; GO:0021915; P:neural tube development; IBA:GO_Central.
DR GO; GO:0051973; P:positive regulation of telomerase activity; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR007604; CP2.
DR Pfam; PF04516; CP2; 1.
DR PROSITE; PS51968; GRH_CP2_DB; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Membrane; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..619
FT /note="Grainyhead-like protein 2 homolog"
FT /id="PRO_0000227996"
FT DOMAIN 239..477
FT /note="Grh/CP2 DB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01313"
FT REGION 1..90
FT /note="Transcription activation"
FT /evidence="ECO:0000250|UniProtKB:Q8K5C0"
FT REGION 86..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 125..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 423..444
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 418
FT /note="Important for activation of transcription"
FT /evidence="ECO:0000250|UniProtKB:Q6ISB3"
FT CONFLICT 488
FT /note="T -> I (in Ref. 1; CAJ83880)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 619 AA; 70451 MW; E921D7A0D3620A75 CRC64;
MSQETDNKRL VVLVPNDAVF NPRRAYTSED EAWKSYLENP LTAATKAMMS INGDEESAAA
IGLLYDYYKV PREKRLLSLN KINEGHEDQD KRNCLPANET PSNLSTGENR VQVLKTVPVN
LSLNNDTVES SNREKYTTSL SESPQPAPAS AVTVVKAEEF TPVFMAPSVP YRSDGEEPRG
VIFEQTHFGV HSITTHSDYL KDDQRSTPDS TYNESFKETT EKYRTPSVGT EEFLYEQTAS
STFQYTLEAT KSLRQKQGEG PMTYLNKGQF YAITLSETGA NKCFRHPISK VRSVIMVVFS
EDKNRDEQLK YWKYWHSRQH TAKQRVLDIA DYKESFNTIG NIEEIAYNAV SFTWDVNEEA
KIFITVNCLS TDFSSQKGVK GLPLMIQIDT YSYNNRSNKP IHRAYCQIKV FCDKGAERKI
RDEERKQNRK KGKSQAAQAQ CNNSADGKLS AVPLQKKSDI TFFKTMTDLD VQPVLFIPDV
HFGNLQRTGQ VFYNTDDDIE GGVLVKRLLR PVDEDYGPPA PKQMKEGSRK VLLYVRKETD
EVFDALMLKY PTVKGLLEAI SEKYGIPVEK IVKIYKKSKK GILVNMDDNI IEHYSNEDTF
ILNVESLAEQ GYKITLTEI
