GRIM_DROME
ID GRIM_DROME Reviewed; 138 AA.
AC Q24570; Q9VVP6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Cell death protein Grim;
GN Name=grim; ORFNames=CG4345;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=Canton-S;
RX PubMed=8698237; DOI=10.1101/gad.10.14.1773;
RA Chen P., Nordstrom W., Gish B., Abrams J.M.;
RT "Grim, a novel cell death gene in Drosophila.";
RL Genes Dev. 10:1773-1782(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION.
RC TISSUE=Embryo;
RX PubMed=9740659; DOI=10.1006/dbio.1998.9000;
RA Chen P., Rodriguez A., Erskine R., Thach T., Abrams J.M.;
RT "Dredd, a novel effector of the apoptosis activators reaper, grim, and hid
RT in Drosophila.";
RL Dev. Biol. 201:202-216(1998).
RN [6]
RP INTERACTION WITH DIAP2.
RX PubMed=18166655; DOI=10.1083/jcb.200706027;
RA Ribeiro P.S., Kuranaga E., Tenev T., Leulier F., Miura M., Meier P.;
RT "DIAP2 functions as a mechanism-based regulator of drICE that contributes
RT to the caspase activity threshold in living cells.";
RL J. Cell Biol. 179:1467-1480(2007).
CC -!- FUNCTION: Activator of apoptosis, independent of rpr and hid, that acts
CC on the effector Dredd. {ECO:0000269|PubMed:8698237,
CC ECO:0000269|PubMed:9740659}.
CC -!- SUBUNIT: Interacts with Diap2 (via BIR2 domain).
CC {ECO:0000269|PubMed:18166655}.
CC -!- DEVELOPMENTAL STAGE: Expression coincides with the onset of programmed
CC cell death (PCD) at all stages of embryonic development.
CC {ECO:0000269|PubMed:8698237}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U61976; AAC47727.1; -; mRNA.
DR EMBL; AE014296; AAF49265.1; -; Genomic_DNA.
DR EMBL; BT003512; AAO39516.1; -; mRNA.
DR RefSeq; NP_524137.2; NM_079413.3.
DR PDB; 1JD5; X-ray; 1.90 A; B=2-11.
DR PDB; 1SE0; X-ray; 1.75 A; B=2-11.
DR PDBsum; 1JD5; -.
DR PDBsum; 1SE0; -.
DR AlphaFoldDB; Q24570; -.
DR SMR; Q24570; -.
DR BioGRID; 65298; 58.
DR DIP; DIP-20419N; -.
DR ELM; Q24570; -.
DR IntAct; Q24570; 2.
DR STRING; 7227.FBpp0074896; -.
DR PaxDb; Q24570; -.
DR EnsemblMetazoa; FBtr0075130; FBpp0074896; FBgn0015946.
DR GeneID; 40014; -.
DR KEGG; dme:Dmel_CG4345; -.
DR UCSC; CG4345-RA; d. melanogaster.
DR CTD; 40014; -.
DR FlyBase; FBgn0015946; grim.
DR VEuPathDB; VectorBase:FBgn0015946; -.
DR eggNOG; ENOG502TD6Q; Eukaryota.
DR HOGENOM; CLU_1857348_0_0_1; -.
DR InParanoid; Q24570; -.
DR OMA; RQQGAMS; -.
DR OrthoDB; 1560536at2759; -.
DR PhylomeDB; Q24570; -.
DR BioGRID-ORCS; 40014; 0 hits in 1 CRISPR screen.
DR EvolutionaryTrace; Q24570; -.
DR GenomeRNAi; 40014; -.
DR PRO; PR:Q24570; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0015946; Expressed in anterior ectoderm anlage (Drosophila) and 19 other tissues.
DR ExpressionAtlas; Q24570; baseline.
DR Genevisible; Q24570; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005739; C:mitochondrion; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:FlyBase.
DR GO; GO:0042803; F:protein homodimerization activity; IPI:FlyBase.
DR GO; GO:0006915; P:apoptotic process; IDA:FlyBase.
DR GO; GO:0097190; P:apoptotic signaling pathway; IGI:FlyBase.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:FlyBase.
DR GO; GO:0035193; P:larval central nervous system remodeling; TAS:FlyBase.
DR GO; GO:0035006; P:melanization defense response; IMP:FlyBase.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; IMP:FlyBase.
DR GO; GO:0043065; P:positive regulation of apoptotic process; IMP:FlyBase.
DR GO; GO:2001272; P:positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis; IDA:FlyBase.
DR GO; GO:1901216; P:positive regulation of neuron death; IMP:FlyBase.
DR GO; GO:0043068; P:positive regulation of programmed cell death; IMP:FlyBase.
DR GO; GO:0010623; P:programmed cell death involved in cell development; IMP:FlyBase.
DR DisProt; DP02149; -.
PE 1: Evidence at protein level;
KW 3D-structure; Apoptosis; Reference proteome.
FT CHAIN 1..138
FT /note="Cell death protein Grim"
FT /id="PRO_0000083848"
FT REGION 21..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 93
FT /note="F -> L (in Ref. 1; AAC47727)"
FT /evidence="ECO:0000305"
FT STRAND 3..6
FT /evidence="ECO:0007829|PDB:1SE0"
SQ SEQUENCE 138 AA; 15486 MW; DE3FAC0487F0DB4E CRC64;
MAIAYFIPDQ AQLLARSYQQ NGQQTAASPR TTATAAAPSQ QQQQSQQQQQ QQRHHHQQQR
PQFRANISVP LGSQQGSMTM SEFGCWDLLA QIFCYALRIY SYSSSQRQPT VIQISFEISS
GGQNNDEDDV TDATSKEN
