GRIN1_HUMAN
ID GRIN1_HUMAN Reviewed; 1008 AA.
AC Q7Z2K8; C9JM70; Q8ND74; Q96PZ4;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=G protein-regulated inducer of neurite outgrowth 1;
DE Short=GRIN1;
GN Name=GPRIN1; Synonyms=KIAA1893;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT VAL-300.
RC TISSUE=Brain;
RX PubMed=11572484; DOI=10.1093/dnares/8.4.179;
RA Nagase T., Kikuno R., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XXI. The
RT complete sequences of 60 new cDNA clones from brain which code for large
RT proteins.";
RL DNA Res. 8:179-187(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANTS THR-121
RP AND VAL-300.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 183-897 (ISOFORM 1), AND VARIANT
RP VAL-300.
RC TISSUE=Brain;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=10480904; DOI=10.1074/jbc.274.38.26931;
RA Chen L.T., Gilman A.G., Kozasa T.;
RT "A candidate target for G protein action in brain.";
RL J. Biol. Chem. 274:26931-26938(1999).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-60; SER-64; SER-75; SER-436;
RP SER-452; SER-615; SER-737 AND SER-993, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: May be involved in neurite outgrowth. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with activated forms of GNAI1, GNAO1 and GNAZ.
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q7Z2K8; Q05941: Chrna7; Xeno; NbExp=6; IntAct=EBI-11152848, EBI-79422;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor
CC {ECO:0000250}. Cell projection, growth cone {ECO:0000250}. Note=Highly
CC enriched in growth cone. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7Z2K8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7Z2K8-2; Sequence=VSP_018546, VSP_018547;
CC -!- TISSUE SPECIFICITY: Widely expressed in the central nervous system,
CC with highest levels in spinal cord. {ECO:0000269|PubMed:10480904}.
CC -!- PTM: Palmitoylation on Cys-999 and/or Cys-1000 is required for membrane
CC targeting. {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB67786.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB067480; BAB67786.1; ALT_INIT; mRNA.
DR EMBL; AC091934; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC052950; AAH52950.1; -; mRNA.
DR EMBL; AL834165; CAD38868.1; -; mRNA.
DR CCDS; CCDS4405.1; -. [Q7Z2K8-1]
DR RefSeq; NP_443131.2; NM_052899.2. [Q7Z2K8-1]
DR AlphaFoldDB; Q7Z2K8; -.
DR BioGRID; 125349; 62.
DR IntAct; Q7Z2K8; 19.
DR MINT; Q7Z2K8; -.
DR STRING; 9606.ENSP00000305839; -.
DR ChEMBL; CHEMBL5266; -.
DR GlyGen; Q7Z2K8; 2 sites, 1 O-linked glycan (2 sites).
DR iPTMnet; Q7Z2K8; -.
DR PhosphoSitePlus; Q7Z2K8; -.
DR SwissPalm; Q7Z2K8; -.
DR BioMuta; GPRIN1; -.
DR DMDM; 296434528; -.
DR EPD; Q7Z2K8; -.
DR jPOST; Q7Z2K8; -.
DR MassIVE; Q7Z2K8; -.
DR MaxQB; Q7Z2K8; -.
DR PaxDb; Q7Z2K8; -.
DR PeptideAtlas; Q7Z2K8; -.
DR PRIDE; Q7Z2K8; -.
DR ProteomicsDB; 68962; -. [Q7Z2K8-1]
DR ProteomicsDB; 68963; -. [Q7Z2K8-2]
DR Antibodypedia; 45999; 122 antibodies from 24 providers.
DR DNASU; 114787; -.
DR Ensembl; ENST00000303991.5; ENSP00000305839.4; ENSG00000169258.7. [Q7Z2K8-1]
DR GeneID; 114787; -.
DR KEGG; hsa:114787; -.
DR MANE-Select; ENST00000303991.5; ENSP00000305839.4; NM_052899.3; NP_443131.2.
DR UCSC; uc003meo.2; human. [Q7Z2K8-1]
DR CTD; 114787; -.
DR DisGeNET; 114787; -.
DR GeneCards; GPRIN1; -.
DR HGNC; HGNC:24835; GPRIN1.
DR HPA; ENSG00000169258; Tissue enhanced (brain, pituitary gland, retina).
DR MIM; 611239; gene.
DR neXtProt; NX_Q7Z2K8; -.
DR OpenTargets; ENSG00000169258; -.
DR PharmGKB; PA162390183; -.
DR VEuPathDB; HostDB:ENSG00000169258; -.
DR eggNOG; ENOG502S6S7; Eukaryota.
DR GeneTree; ENSGT00940000162796; -.
DR HOGENOM; CLU_346336_0_0_1; -.
DR InParanoid; Q7Z2K8; -.
DR OMA; TKAPSWE; -.
DR OrthoDB; 612273at2759; -.
DR PhylomeDB; Q7Z2K8; -.
DR TreeFam; TF337047; -.
DR PathwayCommons; Q7Z2K8; -.
DR Reactome; R-HSA-9022699; MECP2 regulates neuronal receptors and channels.
DR SignaLink; Q7Z2K8; -.
DR SIGNOR; Q7Z2K8; -.
DR BioGRID-ORCS; 114787; 8 hits in 1074 CRISPR screens.
DR ChiTaRS; GPRIN1; human.
DR GenomeRNAi; 114787; -.
DR Pharos; Q7Z2K8; Tbio.
DR PRO; PR:Q7Z2K8; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q7Z2K8; protein.
DR Bgee; ENSG00000169258; Expressed in cortical plate and 120 other tissues.
DR Genevisible; Q7Z2K8; HS.
DR GO; GO:0030426; C:growth cone; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0051219; F:phosphoprotein binding; IEA:Ensembl.
DR GO; GO:0031175; P:neuron projection development; IBA:GO_Central.
DR InterPro; IPR026646; GPRIN2-like/GPRIN3.
DR InterPro; IPR032745; GRIN_C.
DR PANTHER; PTHR15718; PTHR15718; 2.
DR Pfam; PF15235; GRIN_C; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cell projection; Lipoprotein;
KW Membrane; Palmitate; Phosphoprotein; Reference proteome.
FT CHAIN 1..1008
FT /note="G protein-regulated inducer of neurite outgrowth 1"
FT /id="PRO_0000235977"
FT REGION 1..859
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 899..1008
FT /note="Interaction with GNAO1"
FT /evidence="ECO:0000250"
FT REGION 943..986
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..57
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 95..136
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 137..158
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 233..267
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 387..410
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 457..471
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 472..495
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 716..730
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 60
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 64
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 75
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 237
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3UNH4"
FT MOD_RES 436
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 452
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 615
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 737
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 799
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3UNH4"
FT MOD_RES 877
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q3UNH4"
FT MOD_RES 895
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3UNH4"
FT MOD_RES 914
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3UNH4"
FT MOD_RES 993
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT LIPID 999
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 1000
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
FT VAR_SEQ 748..798
FT /note="VEPKAEPVSSTEASSLGQKDLEAAGAERSPCPEAAAPPPGPRTRDNFTKAP
FT -> QASARPVPRAAAECAPAAVLLAGGTHGRVICPHFVRPSFRPSQAPFLITGP (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:11572484"
FT /id="VSP_018546"
FT VAR_SEQ 799..1008
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11572484"
FT /id="VSP_018547"
FT VARIANT 121
FT /note="P -> T (in dbSNP:rs17854765)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_063136"
FT VARIANT 300
FT /note="M -> V (in dbSNP:rs6556276)"
FT /evidence="ECO:0000269|PubMed:11572484,
FT ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:17974005"
FT /id="VAR_063137"
FT VARIANT 337
FT /note="G -> A (in dbSNP:rs10037225)"
FT /id="VAR_056902"
FT VARIANT 357
FT /note="S -> P (in dbSNP:rs34285890)"
FT /id="VAR_056903"
FT CONFLICT 231..238
FT /note="Missing (in Ref. 4; CAD38868)"
FT /evidence="ECO:0000305"
FT CONFLICT 680
FT /note="P -> A (in Ref. 4; CAD38868)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1008 AA; 102399 MW; DA4AAED9917E570E CRC64;
MDTAEDPAWL QLLQKDSSPP GPRPTAFFCP QDGSLGAGSS AMRDYCPSQQ KASPAPPRHT
PDQSPGMESR HRSPSGAGEG ASCSDGPRGS LACPSPTCFS PQESPSKETL EAHGASISGT
PEATTSGKPE PVSSVKTEPK SSDDRNPMFL EKMDFKSSKQ ADSTSIGKED PGSSRKADPM
FTGKAEPEIL GKGDPVAPGR MDPMTVRKED LGSLGKVDPL CSSKTYTVSP RKEDPGSLRK
VDPVSSDKVD PVFPRKEEPR YSGKEHPVSS EKVAPTSAEK VDLVLSGKRD PGPSGKADPM
PLESMDSAST GKTEPGLLGK LIPGSSGKNG PVSSGTGAPG SLGRLDPTCL GMADPASVGN
VETVPATKED SRFLGKMDPA SSGEGRPVSG HTDTTASAKT DLTSLKNVDP MSSGKVDPVS
LGKMDPMCSG KPELLSPGQA ERVSVGKAGT VSPGKEDPVS SRREDPISAG SRKTSSEKVN
PESSGKTNPV SSGPGDPRSL GTAGPPSAVK AEPATGGKGD PLSSEKAGLV ASGKAAPTAS
GKAEPLAVGK EDPVSKGKAD AGPSGQGDSV SIGKVVSTPG KTVPVPSGKV DPVSLGKAEA
IPEGKVGSLP LEKGSPVTTT KADPRASGKA QPQSGGKAET KLPGQEGAAA PGEAGAVCLK
KETPQASEKV DPGSCRKAEP LASGKGEPVS LGKADSAPSR KTESPSLGKV VPLSLEKTKP
SSSSRQLDRK ALGSARSPEG ARGSEGRVEP KAEPVSSTEA SSLGQKDLEA AGAERSPCPE
AAAPPPGPRT RDNFTKAPSW EASAPPPPRE DAGTQAGAQA CVSVAVSPMS PQDGAGGSAF
SFQAAPRAPS PPSRRDAGLQ VSLGAAETRS VATGPMTPQA AAPPAFPEVR VRPGSALAAA
VAPPEPAEPV RDVSWDEKGM TWEVYGAAME VEVLGMAIQK HLERQIEEHG RQGAPAPPPA
ARAGPGRSGS VRTAPPDGAA KRPPGLFRAL LQSVRRPRCC SRAGPTAE
