GRLB_DICDI
ID GRLB_DICDI Reviewed; 755 AA.
AC Q86HH3; Q55AP2;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Metabotropic glutamate receptor-like protein B;
DE Flags: Precursor;
GN Name=grlB; ORFNames=DDB_G0271686;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP NOMENCLATURE.
RX PubMed=16735079; DOI=10.1016/j.ejcb.2006.04.003;
RA Prabhu Y., Eichinger L.;
RT "The Dictyostelium repertoire of seven transmembrane domain receptors.";
RL Eur. J. Cell Biol. 85:937-946(2006).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=17501984; DOI=10.1186/1471-213x-7-44;
RA Prabhu Y., Mueller R., Anjard C., Noegel A.A.;
RT "GrlJ, a Dictyostelium GABAB-like receptor with roles in post-aggregation
RT development.";
RL BMC Dev. Biol. 7:44-44(2007).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Increased levels found from the tight aggregation
CC stage onward. Levels stayed high during late development. Clear
CC expression at 24 hours when fruiting body formation is close to
CC completion. {ECO:0000269|PubMed:17501984}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the BMP lipoprotein
CC family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the G-protein coupled
CC receptor 3 family. GABA-B receptor subfamily. {ECO:0000305}.
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DR EMBL; AAFI02000006; EAL71533.1; -; Genomic_DNA.
DR RefSeq; XP_645480.1; XM_640388.1.
DR AlphaFoldDB; Q86HH3; -.
DR SMR; Q86HH3; -.
DR STRING; 44689.DDB0231970; -.
DR PaxDb; Q86HH3; -.
DR EnsemblProtists; EAL71533; EAL71533; DDB_G0271686.
DR GeneID; 8618108; -.
DR KEGG; ddi:DDB_G0271686; -.
DR dictyBase; DDB_G0271686; grlB.
DR eggNOG; KOG1055; Eukaryota.
DR HOGENOM; CLU_365408_0_0_1; -.
DR InParanoid; Q86HH3; -.
DR OMA; YASHVIN; -.
DR PhylomeDB; Q86HH3; -.
DR PRO; PR:Q86HH3; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:dictyBase.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0016917; F:GABA receptor activity; IMP:dictyBase.
DR GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR InterPro; IPR000337; GPCR_3.
DR InterPro; IPR017978; GPCR_3_C.
DR InterPro; IPR028082; Peripla_BP_I.
DR InterPro; IPR003760; PnrA-like.
DR Pfam; PF00003; 7tm_3; 1.
DR Pfam; PF02608; Bmp; 1.
DR PRINTS; PR00248; GPCRMGR.
DR SUPFAM; SSF53822; SSF53822; 1.
DR PROSITE; PS50259; G_PROTEIN_RECEP_F3_4; 1.
PE 2: Evidence at transcript level;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Signal; Transducer; Transmembrane; Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..755
FT /note="Metabotropic glutamate receptor-like protein B"
FT /id="PRO_0000370347"
FT TOPO_DOM 24..385
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 386..406
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 407..417
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 439..455
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 456..476
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 477..492
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 493..513
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 514..541
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 542..562
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 563..578
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 579..599
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 600..607
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 608..628
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 629..755
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 656..676
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 691..729
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 659..675
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 16
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 183
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 273
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 755 AA; 84118 MW; 4608C2B72361E4D7 CRC64;
MKNLISIILL ILIFFNYSKF VKSKNCKIAV LLSGDFSDLG YNYQFNEARV KAESILKLSD
FSLYYKNLDE SEELSEASFK DAIAQGANLI VVASSGQTEM GLKYAKLYKD SDIYWIIKTS
ARPVDYLPKV AVLDFNTDRA HFTLGYFAGK MSKTGVVGFV SPGAYIASNG NAFYLGAKEA
RSNITFVTTF TGSWFNPEVA YKAAEMLISN GADFLGMSQD DMSVQKAVID SGSLGLGVTG
YPTRLIYGSA NIGLSYITDW TDVFVKYASH VINDTWPDSD YYKTSLNPGG SLLFDEYSYL
VPQSIRDLVN NEIEILKNDD FNPFRCNPMY IDLGYKLDVN GCISYNEFEK NQQVLKGDKY
SKTISFGTYT IPIEFVDYSS SMKLGITITS SICIFLCIIS IIIVLVFRTA RIIKSASPAF
LFLILMGCIL IFIGCIIFSQ SPNEGTCRAR VWLLSIGYTI FLGSLLVKNW RIWLLFDNPK
LKKRSITNWK LYPWVAGILA ADVLILAFWQ GLGNIRSESR IGIDSLTKYQ YTNVCSSNDQ
GSIALYILLV FHGIKLLVAC FISFKIKVVD IDEFNESKPI ASSVYIITFC LFIVIPLMVS
PQSVTSQVTT ICVCAIVTTL ISIILLFGSK FYKMITQGAA LNQTFASSSK SSSFSQSLEK
KKTGEEDDSE SSEENGKKAI VVTQQSVLAH FSSDTEDDEN ETQQIDEEKD EQIAGSNEDI
IQPEENIEEN NVSVIQSKRL SNQLNGEVEI DSNNL
