GRLE_DICDI
ID GRLE_DICDI Reviewed; 816 AA.
AC Q54ET0; Q1PDD1;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 2.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Metabotropic glutamate receptor-like protein E;
DE AltName: Full=DdmGluPR;
DE AltName: Full=GABA-B receptor-like protein grlE;
DE Flags: Precursor;
GN Name=grlE; Synonyms=GluPR; ORFNames=DDB_G0291356;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=AX2;
RX PubMed=16527814; DOI=10.1074/jbc.m512723200;
RA Taniura H., Sanada N., Kuramoto N., Yoneda Y.;
RT "A metabotropic glutamate receptor family gene in Dictyostelium
RT discoideum.";
RL J. Biol. Chem. 281:12336-12343(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP DISRUPTION PHENOTYPE, FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=16672332; DOI=10.1242/dev.02399;
RA Anjard C., Loomis W.F.;
RT "GABA induces terminal differentiation of Dictyostelium through a GABAB
RT receptor.";
RL Development 133:2253-2261(2006).
RN [4]
RP NOMENCLATURE.
RX PubMed=16735079; DOI=10.1016/j.ejcb.2006.04.003;
RA Prabhu Y., Eichinger L.;
RT "The Dictyostelium repertoire of seven transmembrane domain receptors.";
RL Eur. J. Cell Biol. 85:937-946(2006).
RN [5]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=17501984; DOI=10.1186/1471-213x-7-44;
RA Prabhu Y., Mueller R., Anjard C., Noegel A.A.;
RT "GrlJ, a Dictyostelium GABAB-like receptor with roles in post-aggregation
RT development.";
RL BMC Dev. Biol. 7:44-44(2007).
RN [6]
RP INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=18559084; DOI=10.1186/1471-2164-9-291;
RA Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B.,
RA Skelton J., Ivens A., Bozzaro S.;
RT "Genome-wide transcriptional changes induced by phagocytosis or growth on
RT bacteria in Dictyostelium.";
RL BMC Genomics 9:291-291(2008).
CC -!- FUNCTION: May be involved in early development in cAMP sensing and
CC subsequent chemotactic response. Probable receptor of GABA and
CC glutamate, leading respectively to the induction or inhibition of SDF-2
CC formation. {ECO:0000269|PubMed:16672332, ECO:0000269|PubMed:17501984}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:16527814}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:16527814}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development. Expression is
CC very low in vegetative cells and starts to accumulate in the first 2
CC hours after the initiation of development. It reaches a peak at 4 hours
CC and then declines. However, a slightly larger transcript accumulates
CC after 10 hours and remains until 22 hours of development.
CC {ECO:0000269|PubMed:16527814, ECO:0000269|PubMed:16672332,
CC ECO:0000269|PubMed:17501984}.
CC -!- INDUCTION: Down-regulated by growth on bacteria.
CC {ECO:0000269|PubMed:18559084}.
CC -!- DISRUPTION PHENOTYPE: Cells grow faster, reach higher densities,
CC exhibit delayed aggregates formation upon starvation and altered
CC chemotaxis toward cAMP. Cells do not produce SDF-2 in response to GABA.
CC {ECO:0000269|PubMed:16527814, ECO:0000269|PubMed:16672332}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 3 family.
CC {ECO:0000305}.
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DR EMBL; DQ447637; ABE02692.1; -; mRNA.
DR EMBL; AAFI02000177; EAL61662.1; -; Genomic_DNA.
DR RefSeq; XP_635160.1; XM_630068.1.
DR AlphaFoldDB; Q54ET0; -.
DR SMR; Q54ET0; -.
DR STRING; 44689.DDB0231976; -.
DR TCDB; 9.A.14.15.3; the g-protein-coupled receptor (gpcr) family.
DR PaxDb; Q54ET0; -.
DR EnsemblProtists; EAL61662; EAL61662; DDB_G0291356.
DR GeneID; 8628106; -.
DR KEGG; ddi:DDB_G0291356; -.
DR dictyBase; DDB_G0291356; grlE.
DR eggNOG; KOG1055; Eukaryota.
DR InParanoid; Q54ET0; -.
DR PhylomeDB; Q54ET0; -.
DR Reactome; R-DDI-418594; G alpha (i) signalling events.
DR Reactome; R-DDI-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-DDI-977444; GABA B receptor activation.
DR PRO; PR:Q54ET0; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0038039; C:G protein-coupled receptor heterodimeric complex; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IDA:dictyBase.
DR GO; GO:0004965; F:G protein-coupled GABA receptor activity; IMP:dictyBase.
DR GO; GO:0008066; F:glutamate receptor activity; IMP:dictyBase.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IBA:GO_Central.
DR GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0043327; P:chemotaxis to cAMP; IMP:dictyBase.
DR GO; GO:0007214; P:gamma-aminobutyric acid signaling pathway; IMP:dictyBase.
DR InterPro; IPR001828; ANF_lig-bd_rcpt.
DR InterPro; IPR002455; GPCR3_GABA-B.
DR InterPro; IPR000337; GPCR_3.
DR InterPro; IPR017978; GPCR_3_C.
DR InterPro; IPR028082; Peripla_BP_I.
DR PANTHER; PTHR10519; PTHR10519; 1.
DR Pfam; PF00003; 7tm_3; 1.
DR Pfam; PF01094; ANF_receptor; 1.
DR PRINTS; PR00248; GPCRMGR.
DR SUPFAM; SSF53822; SSF53822; 1.
DR PROSITE; PS50259; G_PROTEIN_RECEP_F3_4; 1.
PE 2: Evidence at transcript level;
KW Chemotaxis; G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Signal; Transducer; Transmembrane; Transmembrane helix.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..816
FT /note="Metabotropic glutamate receptor-like protein E"
FT /id="PRO_0000328377"
FT TOPO_DOM 28..436
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 437..457
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 458..469
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 470..490
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 491..496
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 497..517
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 518..541
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 542..562
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 563..590
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 591..611
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 612..625
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 626..646
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 647..653
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 654..674
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 675..816
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 697..718
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 68
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 311
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 388
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 816 AA; 89935 MW; 810B7050E872C51D CRC64;
MKIKIGNILK NVVILVIFSL FISKINSEVV KPNPAKPDEL VIAFMSPYFD PEYTQYSGAA
EYALSKFNET FKTKYSKTIK LHTFTDTNDV IDSLDLVTMP VNGIVGPAYS GSSSTACLVF
GAFAVPSISF YATGASLSNS GSYPYFQRVM PDDRLQVQAI LSLLKKNGWT RVSCIHTNED
YGNGGADQLV QQSNAQGITV NTNQAIDPVD NGIAPEQLYY DIVFDNLEAA KSRVIVLYAL
FPPDCLEIWK QAKARGFLGE GFTWIVTDGC AELTGGTDPD LLGVLATFPS YGLGTEAYTD
FERTIVNDYN NNTGDAFYKG ASFSYDATYA MLMGIEAVLA KGGDPWDGEQ VRTELRNLKF
NGITGTIAFD KNTGDRLYGE FALLNLINST KGSFDPIGKI NPDSGEITLK SDILYSGPTY
KVPSDYQVVV FDRTLNIVLG VITGVCVLIV IGIGSVIALQ WRKFRYSSPL FCMFIIIGAL
MGLASVFTLL PTPTTPLCSG FPWLLGLGYV IVFGTLFTKT WRTWRLFSNA RKFKIIRITN
KFIITLVGGF VLLESIFMII WTAVDRPIPL AEPIFKAGEA QLQCTSDSEA WWYVFVFYKV
FYILFGVFLA FKTRNVVDSL NESKPITLAL YNLTFVMVVA IALGFILRDN PIAIIVIQTI
AILLGFTVTV SVLFLPKVWM ILSGQQHSMD SIGTSMDSMG RSNGNTTEAE STRGYTNKDY
NNQSVGRSFS AHATGFKDVA PSNHPQLGIV YTGGDNFPRV GSSQTAASRS EAEINVSKDV
LAKANKKNEE EFGEFFLKPS EDNILKKKKK KKNNNK
