GRLF_DICDI
ID GRLF_DICDI Reviewed; 770 AA.
AC Q54SW3;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Metabotropic glutamate receptor-like protein F;
DE Flags: Precursor;
GN Name=grlF; ORFNames=DDB_G0282175;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NOMENCLATURE.
RX PubMed=16735079; DOI=10.1016/j.ejcb.2006.04.003;
RA Prabhu Y., Eichinger L.;
RT "The Dictyostelium repertoire of seven transmembrane domain receptors.";
RL Eur. J. Cell Biol. 85:937-946(2006).
RN [3]
RP DEVELOPMENTAL STAGE.
RX PubMed=17501984; DOI=10.1186/1471-213x-7-44;
RA Prabhu Y., Mueller R., Anjard C., Noegel A.A.;
RT "GrlJ, a Dictyostelium GABAB-like receptor with roles in post-aggregation
RT development.";
RL BMC Dev. Biol. 7:44-44(2007).
RN [4]
RP INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=18590548; DOI=10.1186/1471-2180-8-109;
RA Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R.,
RA Ivens A., Martinez J.L., Escalante R.;
RT "Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common
RT and specific effects from PAO1 and PA14 strains.";
RL BMC Microbiol. 8:109-109(2008).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Increased levels found from the tight aggregation
CC stage onward. Levels stayed high during late development. Clear
CC expression at 24 hours when fruiting body formation is close to
CC completion. {ECO:0000269|PubMed:17501984}.
CC -!- INDUCTION: Down-regulated by Pseudomonas aeruginosa, PAO1 strain and
CC PA14 strain infection. {ECO:0000269|PubMed:18590548}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the BMP lipoprotein
CC family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the G-protein coupled
CC receptor 3 family. GABA-B receptor subfamily. {ECO:0000305}.
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DR EMBL; AAFI02000045; EAL66367.1; -; Genomic_DNA.
DR RefSeq; XP_640346.1; XM_635254.1.
DR AlphaFoldDB; Q54SW3; -.
DR SMR; Q54SW3; -.
DR PaxDb; Q54SW3; -.
DR EnsemblProtists; EAL66367; EAL66367; DDB_G0282175.
DR GeneID; 8623446; -.
DR KEGG; ddi:DDB_G0282175; -.
DR dictyBase; DDB_G0282175; grlF.
DR eggNOG; KOG1055; Eukaryota.
DR HOGENOM; CLU_365408_0_0_1; -.
DR InParanoid; Q54SW3; -.
DR PhylomeDB; Q54SW3; -.
DR PRO; PR:Q54SW3; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR InterPro; IPR017978; GPCR_3_C.
DR InterPro; IPR003760; PnrA-like.
DR Pfam; PF00003; 7tm_3; 1.
DR Pfam; PF02608; Bmp; 1.
DR PROSITE; PS50259; G_PROTEIN_RECEP_F3_4; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Signal; Transducer; Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..770
FT /note="Metabotropic glutamate receptor-like protein F"
FT /id="PRO_0000370350"
FT TOPO_DOM 23..370
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..391
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 392..405
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 406..426
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 427..442
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 443..463
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 464..483
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 484..504
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 505..528
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 529..549
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 550..565
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 566..586
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 587..594
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 595..615
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 616..770
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 639..740
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 715..760
FT /evidence="ECO:0000255"
FT COMPBIAS 639..658
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 672..698
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 699..725
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 726..740
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 24
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 185
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 260
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 286
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 319
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 344
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 770 AA; 86464 MW; 5407FA6FC64ED57F CRC64;
MKIKNFIYFL IYFIFLFKVI NGQNKTCKIS VLLSGDKSDL GYNYMMNEAR VLAESQLHMY
PFSIYYEHLE ESTLEAEHAI QDSVDKGANL IVVSSVIHFS LGVKYATKYK DEPIYWIIRG
SKLVPTLPKV VILNFNSFEL HYLLGYYAGL ATKTGVVGFV SPGIGINTLS CDNSFYIGAK
YARSNITFLD IHTGSWYNPE VSYKAAQKLI ENGADVIGMS QDDMSVQKAI MDSGGLGLGV
TGYPGHYQFG SDVAYSYLTN WTNLFITYAN HVINDDWPAF DSFFTNLSRK DAIFMDDFSF
RVPIDIQTKT KIELENLMNT SYAPYKSDPI LESIGLQFSG NWINDTQFRA NTKILPSYGD
STVDYPESLK IGVTVVSGFC IFLCLISMII VIKFKEAKVI KSSSPIFCLL ILFGCIVIFV
GCIMFARSPT DGSCRSRVWL LSLGYTIFLG NLMVKNWRIW LLFDNPKLKK RAITNWKLYP
WVSGIVIIDI VILSIWQALG DIVAESRTGI DSLTKYEYRN VCASSDQGSI ALYLLLVFHG
LILLVACFIS FKIKVVDIEE FNESKPITTS VYIITFCLFI VIPIMVSSPT VTTQTTIICI
CALITTMLSI ILLFGTKFFK MITVGLELGQ TFVTSTKSSS HSQRTKSSKS SNGSGPKING
FGGNVLNLDD DSDEISSEKE KKKPIESNPK DHMAVFTSDE ETSKASKFSS EQFSREQIND
NIILENNNDN EEKQKDEEEI KEEKLLVSEI QAKRLSLEQN GQTEIDSNDV
