ID   GRLO_DICDI              Reviewed;         819 AA.
AC   Q55AP3; Q75JA3; Q75JD0;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Metabotropic glutamate receptor-like protein O;
DE   Flags: Precursor;
GN   Name=grlO; ORFNames=DDB_G0271688;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=16735079; DOI=10.1016/j.ejcb.2006.04.003;
RA   Prabhu Y., Eichinger L.;
RT   "The Dictyostelium repertoire of seven transmembrane domain receptors.";
RL   Eur. J. Cell Biol. 85:937-946(2006).
RN   [4]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=17501984; DOI=10.1186/1471-213x-7-44;
RA   Prabhu Y., Mueller R., Anjard C., Noegel A.A.;
RT   "GrlJ, a Dictyostelium GABAB-like receptor with roles in post-aggregation
RT   development.";
RL   BMC Dev. Biol. 7:44-44(2007).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Increased levels found from the tight aggregation
CC       stage onward. Levels stayed high during late development. Clear
CC       expression at 24 hours when fruiting body formation is close to
CC       completion. {ECO:0000269|PubMed:17501984}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the BMP lipoprotein
CC       family. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the G-protein coupled
CC       receptor 3 family. GABA-B receptor subfamily. {ECO:0000305}.
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DR   EMBL; AAFI02000006; EAL71534.1; -; Genomic_DNA.
DR   RefSeq; XP_645479.1; XM_640387.1.
DR   AlphaFoldDB; Q55AP3; -.
DR   SMR; Q55AP3; -.
DR   PaxDb; Q55AP3; -.
DR   EnsemblProtists; EAL71534; EAL71534; DDB_G0271688.
DR   GeneID; 8618107; -.
DR   KEGG; ddi:DDB_G0271688; -.
DR   dictyBase; DDB_G0271688; grlO.
DR   eggNOG; KOG1055; Eukaryota.
DR   HOGENOM; CLU_365408_0_0_1; -.
DR   InParanoid; Q55AP3; -.
DR   PhylomeDB; Q55AP3; -.
DR   PRO; PR:Q55AP3; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR017978; GPCR_3_C.
DR   InterPro; IPR003760; PnrA-like.
DR   Pfam; PF00003; 7tm_3; 1.
DR   Pfam; PF02608; Bmp; 1.
DR   PROSITE; PS50259; G_PROTEIN_RECEP_F3_4; 1.
PE   2: Evidence at transcript level;
KW   G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW   Reference proteome; Signal; Transducer; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..819
FT                   /note="Metabotropic glutamate receptor-like protein O"
FT                   /id="PRO_0000370358"
FT   TOPO_DOM        20..394
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        395..415
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        416..428
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        429..449
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        450..457
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        458..478
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        479..503
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        504..524
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        525..550
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        551..571
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        572..587
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        588..608
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        609..624
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        625..645
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        646..819
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          674..819
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        718..741
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        748..766
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        767..796
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        797..819
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        99
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        185
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        277
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        295
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        330
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        370
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   819 AA;  92649 MW;  A18109BE471383A3 CRC64;
     MKKVFFLILI LNCVVGALSN KNICKISLLL SGDYNDIGVN YMFNYARTQV EKNLNINSIV
     FTNLENNQNA INNAIIESIN KGSNFLISTI NSHSNFIINY SRLYKNKEIF WLIKGNDNER
     PIPDDLPRVK ILNINSDLSF YYLGFISSLI SKTGKIGFIS TKNIETDYQR LTNAFYIGAI
     NSNPNITFLV CSNNFNNQNN NKKISYKISK LLISKGVDFI GSDQDDNSIQ LAVIDNGGIG
     LGLTGFEYSK IYNDKFPFSF KLEWSQLLID ITNTIINGSW VDYDIIYITS FSRLNGTTNF
     TPEIEPIINY NFIPKIYQKQ INDEINKLKN HSTNYYFPHL CNNLFNNIYN QKQTNGCITN
     EQFSNSHLLN ASNIKIIDNK EILEFVDSYS NSIKISILSV SIFCIFICVL GMIFITVLRN
     ARILKSSSPS FLLLILFGCI VIFTGCILFS QPATDKTCQG RVWLLSIGYT IFLGSLLIKN
     WRVWLLFDNK KLRKRSITNW KLYPWVAGIL VVDVLILALW QGLGDIKSES RIIGTSFYQY
     TNVCTNNDQG SIALYILLAF HGLKLLGTCF ISFKIKLVDI EEFNESKPIT TSVFIILFCI
     FTIILLIAPS SSSSSASSPQ PIASLETIIC ICSVTTTAIS IGLLFGDKIY FITTQGLGLN
     QTFAKSSSFS LDKKDCDDDD DDSSDGSDHS NSNKNKNKNK NRNQSEKKKR PNSIKPIGLF
     SKSKQESVVF NPPSNNDLTN ELALPIEGIK EGHGHDSENN DEYEHHEDED HEYEGEGEDE
     DHEDEYEVEN DIEQEQEQES SNISISTKKN NENEIISDT