GROA_MOUSE
ID GROA_MOUSE Reviewed; 96 AA.
AC P12850;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Growth-regulated alpha protein;
DE AltName: Full=C-X-C motif chemokine 1;
DE AltName: Full=Platelet-derived growth factor-inducible protein KC;
DE AltName: Full=Secretory protein N51;
DE Contains:
DE RecName: Full=KC(5-72);
DE AltName: Full=Hematopoietic synergistic factor;
DE Short=HSF;
DE AltName: Full=KC-T;
DE Flags: Precursor;
GN Name=Cxcl1; Synonyms=Gro, Gro1, Mgsa, Scyb1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2917992; DOI=10.1016/s0021-9258(19)84972-4;
RA Oquendo P., Alberta J., Wen D., Graycar J.L., Derynck R., Stiles C.D.;
RT "The platelet-derived growth factor-inducible KC gene encodes a secretory
RT protein related to platelet alpha-granule proteins.";
RL J. Biol. Chem. 264:4133-4137(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2909392; DOI=10.1016/0014-4827(89)90230-9;
RA Ryseck R.P., Macdonald-Bravo H., Mattei M.-G., Bravo R.;
RT "Cloning and sequence of a secretory protein induced by growth factors in
RT mouse fibroblasts.";
RL Exp. Cell Res. 180:266-275(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RA Bozic C.R., Kolakowski L.F. Jr., von Uexkull C., Garcia-Rodriguez M.,
RA Conklyn M.J., Breslow R., Showell H.J., Gerard N.P., Gerard C.;
RL Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
RC TISSUE=Liver;
RX PubMed=7561058;
RA Ohmori Y., Fukumoto S., Hamilton T.A.;
RT "Two structurally distinct kappa B sequence motifs cooperatively control
RT LPS-induced KC gene transcription in mouse macrophages.";
RL J. Immunol. 155:3593-3600(1995).
RN [5]
RP PROTEIN SEQUENCE OF 29-46, IDENTIFICATION OF KC(5-72) BY MASS SPECTROMETRY,
RP PROTEOLYTIC PROCESSING OF N-TERMINUS, AND FUNCTION.
RX PubMed=10725737; DOI=10.4049/jimmunol.164.7.3774;
RA King A.G., Johanson K., Frey C.L., DeMarsh P.L., White J.R., McDevitt P.,
RA McNulty D., Balcarek J., Jonak Z.L., Bhatnagar P.K., Pelus L.M.;
RT "Identification of unique truncated KC/GRO beta chemokines with potent
RT hematopoietic and anti-infective activities.";
RL J. Immunol. 164:3774-3782(2000).
CC -!- FUNCTION: Has chemotactic activity for neutrophils. Contributes to
CC neutrophil activation during inflammation (By similarity).
CC Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic
CC progenitor cell proliferation. KC(5-72) shows a highly enhanced
CC hematopoietic activity. {ECO:0000250, ECO:0000269|PubMed:10725737}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- INDUCTION: By platelet-derived growth factor. In lung, by
CC lipopolysaccharide or inflammation (By similarity). {ECO:0000250}.
CC -!- PTM: The N-terminal processed form KC(5-72) is produced by proteolytic
CC cleavage after secretion from bone marrow stromal cells.
CC {ECO:0000269|PubMed:10725737}.
CC -!- SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
CC {ECO:0000305}.
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DR EMBL; J04596; AAA40131.1; -; mRNA.
DR EMBL; U20634; AAB03376.1; -; Genomic_DNA.
DR EMBL; U20527; AAB03376.1; JOINED; Genomic_DNA.
DR EMBL; S79767; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS39143.1; -.
DR PIR; A32954; A32954.
DR RefSeq; NP_032202.1; NM_008176.3.
DR AlphaFoldDB; P12850; -.
DR SMR; P12850; -.
DR IntAct; P12850; 1.
DR MINT; P12850; -.
DR STRING; 10090.ENSMUSP00000031327; -.
DR PaxDb; P12850; -.
DR PRIDE; P12850; -.
DR ProteomicsDB; 271098; -.
DR ABCD; P12850; 3 sequenced antibodies.
DR DNASU; 14825; -.
DR Ensembl; ENSMUST00000031327; ENSMUSP00000031327; ENSMUSG00000029380.
DR GeneID; 14825; -.
DR KEGG; mmu:14825; -.
DR UCSC; uc008ybl.2; mouse.
DR CTD; 2919; -.
DR MGI; MGI:108068; Cxcl1.
DR VEuPathDB; HostDB:ENSMUSG00000029380; -.
DR eggNOG; ENOG502S7MM; Eukaryota.
DR GeneTree; ENSGT00940000155233; -.
DR HOGENOM; CLU_143902_1_0_1; -.
DR InParanoid; P12850; -.
DR OMA; EKMLNCD; -.
DR OrthoDB; 1617719at2759; -.
DR PhylomeDB; P12850; -.
DR TreeFam; TF333433; -.
DR Reactome; R-MMU-380108; Chemokine receptors bind chemokines.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 14825; 0 hits in 74 CRISPR screens.
DR ChiTaRS; Cxcl1; mouse.
DR PRO; PR:P12850; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; P12850; protein.
DR Bgee; ENSMUSG00000029380; Expressed in endothelial cell of lymphatic vessel and 93 other tissues.
DR ExpressionAtlas; P12850; baseline and differential.
DR Genevisible; P12850; MM.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0008009; F:chemokine activity; ISO:MGI.
DR GO; GO:0045236; F:CXCR chemokine receptor binding; IBA:GO_Central.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0002526; P:acute inflammatory response; ISO:MGI.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR GO; GO:0097398; P:cellular response to interleukin-17; IDA:MGI.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IBA:GO_Central.
DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0030593; P:neutrophil chemotaxis; ISO:MGI.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISO:MGI.
DR GO; GO:1902035; P:positive regulation of hematopoietic stem cell proliferation; IDA:UniProtKB.
DR GO; GO:0070965; P:positive regulation of neutrophil mediated killing of fungus; IDA:UniProtKB.
DR GO; GO:0043268; P:positive regulation of potassium ion transport; ISO:MGI.
DR GO; GO:0010765; P:positive regulation of sodium ion transport; ISO:MGI.
DR GO; GO:0032930; P:positive regulation of superoxide anion generation; IDA:UniProtKB.
DR CDD; cd00273; Chemokine_CXC; 1.
DR InterPro; IPR001089; Chemokine_CXC.
DR InterPro; IPR018048; Chemokine_CXC_CS.
DR InterPro; IPR001811; Chemokine_IL8-like_dom.
DR InterPro; IPR033899; CXC_Chemokine_domain.
DR InterPro; IPR036048; Interleukin_8-like_sf.
DR PANTHER; PTHR10179; PTHR10179; 1.
DR Pfam; PF00048; IL8; 1.
DR PRINTS; PR00437; SMALLCYTKCXC.
DR SMART; SM00199; SCY; 1.
DR SUPFAM; SSF54117; SSF54117; 1.
DR PROSITE; PS00471; SMALL_CYTOKINES_CXC; 1.
PE 1: Evidence at protein level;
KW Cytokine; Direct protein sequencing; Disulfide bond; Growth factor;
KW Inflammatory response; Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000305"
FT CHAIN 25..96
FT /note="Growth-regulated alpha protein"
FT /id="PRO_0000005053"
FT CHAIN 29..96
FT /note="KC(5-72)"
FT /id="PRO_0000005054"
FT DISULFID 33..59
FT /evidence="ECO:0000250"
FT DISULFID 35..75
FT /evidence="ECO:0000250"
SQ SEQUENCE 96 AA; 10254 MW; 4A52B5E5C38B45C2 CRC64;
MIPATRSLLC AALLLLATSR LATGAPIANE LRCQCLQTMA GIHLKNIQSL KVLPSGPHCT
QTEVIATLKN GREACLDPEA PLVQKIVQKM LKGVPK
