GROA_RAT
ID GROA_RAT Reviewed; 96 AA.
AC P14095;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Growth-regulated alpha protein;
DE AltName: Full=C-X-C motif chemokine 1;
DE AltName: Full=Cytokine-induced neutrophil chemoattractant 1;
DE Short=CINC-1;
DE AltName: Full=Platelet-derived growth factor-inducible protein KC;
DE Flags: Precursor;
GN Name=Cxcl1; Synonyms=Cinc1, Gro, Scyb1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=8482545; DOI=10.1016/0378-1119(93)90382-d;
RA Konishi K., Tanaka Y., Yamamoto M., Yomogida K., Watanabe K., Tsurufuji S.,
RA Fujioka M.;
RT "Structure of the gene encoding rat neutrophil chemo-attractant Gro.";
RL Gene 126:285-286(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1374243; DOI=10.1016/0006-291x(92)90679-f;
RA Huang S., Paulauskis J.D., Kobzik L.;
RT "Rat KC cDNA cloning and mRNA expression in lung macrophages and
RT fibroblasts.";
RL Biochem. Biophys. Res. Commun. 184:922-929(1992).
RN [3]
RP PROTEIN SEQUENCE OF 25-96.
RX PubMed=2684956; DOI=10.1016/s0021-9258(19)47149-4;
RA Watanabe K., Konishi K., Fujioka M., Kinoshita S., Nakagawa H.;
RT "The neutrophil chemoattractant produced by the rat kidney epithelioid cell
RT line NRK-52E is a protein related to the KC/gro protein.";
RL J. Biol. Chem. 264:19559-19563(1989).
RN [4]
RP PROTEIN SEQUENCE OF 25-56.
RC STRAIN=Wistar;
RX PubMed=8607872; DOI=10.1006/bbrc.1996.0511;
RA Nakagawa H., Shiota S., Takano K., Shibata F., Kato H.;
RT "Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member
RT of rat GRO/CINCs, is a predominant chemokine produced by
RT lipopolysaccharide-stimulated rat macrophages in culture.";
RL Biochem. Biophys. Res. Commun. 220:945-948(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 36-88.
RC TISSUE=Lung;
RX PubMed=1415488;
RA Huang S., Paulauskis J.D., Godleski J.J., Kobzik L.;
RT "Expression of macrophage inflammatory protein-2 and KC mRNA in pulmonary
RT inflammation.";
RL Am. J. Pathol. 141:981-988(1992).
RN [6]
RP STRUCTURE BY NMR.
RX PubMed=7957925; DOI=10.1016/0014-5793(94)01081-1;
RA Hanzawa H., Haruyama H., Watanabe K., Tsurufuji S.;
RT "The three dimensional structure of rat cytokine CINC/Gro in solution by
RT homonuclear 3D NMR.";
RL FEBS Lett. 354:207-212(1994).
RN [7]
RP STRUCTURE BY NMR.
RX PubMed=9192722; DOI=10.1093/oxfordjournals.jbchem.a021662;
RA Hanzawa H., Haruyama H., Konishi K., Watanabe K., Tsurufuji S.;
RT "Subunit association and monomer structure of CINC/Gro revealed by 1H-
RT NMR.";
RL J. Biochem. 121:835-841(1997).
RN [8]
RP STRUCTURE BY NMR.
RX PubMed=9504410; DOI=10.1093/oxfordjournals.jbchem.a021917;
RA Hanzawa H., Haruyama H., Konishi K., Watanabe K., Tsurufuji S.;
RT "Solution structure of CINC/Gro investigated by heteronuclear NMR.";
RL J. Biochem. 123:62-70(1998).
CC -!- FUNCTION: Has chemotactic activity for neutrophils. Contributes to
CC neutrophil activation during inflammation.
CC -!- SUBUNIT: Monomer and homodimer.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: At least expressed in the lung and trachea.
CC -!- INDUCTION: By lipopolysaccharides (LPS) and inflammation.
CC -!- SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
CC {ECO:0000305}.
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DR EMBL; D11445; BAA02009.1; -; Genomic_DNA.
DR EMBL; D11444; BAA02008.1; -; mRNA.
DR EMBL; M86536; AAA42053.1; -; mRNA.
DR EMBL; S45856; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; JN0572; JN0572.
DR RefSeq; NP_110472.1; NM_030845.1.
DR AlphaFoldDB; P14095; -.
DR SMR; P14095; -.
DR STRING; 10116.ENSRNOP00000003778; -.
DR PaxDb; P14095; -.
DR PRIDE; P14095; -.
DR GeneID; 81503; -.
DR KEGG; rno:81503; -.
DR UCSC; RGD:619869; rat.
DR CTD; 2919; -.
DR RGD; 619869; Cxcl1.
DR eggNOG; ENOG502S7MM; Eukaryota.
DR InParanoid; P14095; -.
DR OrthoDB; 1617719at2759; -.
DR PhylomeDB; P14095; -.
DR Reactome; R-RNO-380108; Chemokine receptors bind chemokines.
DR Reactome; R-RNO-418594; G alpha (i) signalling events.
DR Reactome; R-RNO-6798695; Neutrophil degranulation.
DR PRO; PR:P14095; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005615; C:extracellular space; IDA:RGD.
DR GO; GO:0008009; F:chemokine activity; IDA:RGD.
DR GO; GO:0045236; F:CXCR chemokine receptor binding; IBA:GO_Central.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0002526; P:acute inflammatory response; IDA:RGD.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR GO; GO:0097398; P:cellular response to interleukin-17; ISO:RGD.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IBA:GO_Central.
DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0030593; P:neutrophil chemotaxis; IDA:RGD.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IDA:RGD.
DR GO; GO:1902035; P:positive regulation of hematopoietic stem cell proliferation; ISO:RGD.
DR GO; GO:0070965; P:positive regulation of neutrophil mediated killing of fungus; ISO:RGD.
DR GO; GO:0043268; P:positive regulation of potassium ion transport; IDA:RGD.
DR GO; GO:0010765; P:positive regulation of sodium ion transport; IDA:RGD.
DR GO; GO:0032930; P:positive regulation of superoxide anion generation; ISO:RGD.
DR GO; GO:0001975; P:response to amphetamine; IEP:RGD.
DR GO; GO:0032355; P:response to estradiol; IEP:RGD.
DR GO; GO:0010332; P:response to gamma radiation; IEP:RGD.
DR GO; GO:0051384; P:response to glucocorticoid; IEP:RGD.
DR GO; GO:0009408; P:response to heat; IEP:RGD.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
DR CDD; cd00273; Chemokine_CXC; 1.
DR InterPro; IPR001089; Chemokine_CXC.
DR InterPro; IPR018048; Chemokine_CXC_CS.
DR InterPro; IPR001811; Chemokine_IL8-like_dom.
DR InterPro; IPR033899; CXC_Chemokine_domain.
DR InterPro; IPR036048; Interleukin_8-like_sf.
DR PANTHER; PTHR10179; PTHR10179; 1.
DR Pfam; PF00048; IL8; 1.
DR PRINTS; PR00437; SMALLCYTKCXC.
DR SMART; SM00199; SCY; 1.
DR SUPFAM; SSF54117; SSF54117; 1.
DR PROSITE; PS00471; SMALL_CYTOKINES_CXC; 1.
PE 1: Evidence at protein level;
KW Cytokine; Direct protein sequencing; Disulfide bond; Growth factor;
KW Inflammatory response; Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000269|PubMed:2684956,
FT ECO:0000269|PubMed:8607872"
FT CHAIN 25..96
FT /note="Growth-regulated alpha protein"
FT /id="PRO_0000005055"
FT DISULFID 33..59
FT /evidence="ECO:0000250"
FT DISULFID 35..75
FT /evidence="ECO:0000250"
SQ SEQUENCE 96 AA; 10249 MW; 6046C9E2C2582652 CRC64;
MVSATRSLLC AALPVLATSR QATGAPVANE LRCQCLQTVA GIHFKNIQSL KVMPPGPHCT
QTEVIATLKN GREACLDPEA PMVQKIVQKM LKGVPK
