GRP16_ARATH
ID GRP16_ARATH Reviewed; 244 AA.
AC Q9LY08; Q43308; Q94FP7; Q94FP8; Q94FQ5; Q94FR1;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Tapetal oleosin GRP-16 {ECO:0000303|PubMed:11431566};
DE Short=T-oleosin GRP-16 {ECO:0000305};
DE AltName: Full=Glycine-rich protein 16 {ECO:0000303|PubMed:11431566};
DE Short=AtGRP-16 {ECO:0000303|PubMed:11431566};
DE AltName: Full=Glycine-rich protein 6 {ECO:0000303|PubMed:8220457};
DE Short=AtGRP-6 {ECO:0000303|PubMed:8220457};
DE AltName: Full=Oleopollenin GRP-16 {ECO:0000305|PubMed:14739246};
GN Name=GRP16 {ECO:0000303|PubMed:11431566};
GN Synonyms=GRP6 {ECO:0000303|PubMed:8220457};
GN OrderedLocusNames=At5g07540 {ECO:0000312|Araport:AT5G07540};
GN ORFNames=T2I1.250 {ECO:0000312|EMBL:CAB87943.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2), AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Columbia; TISSUE=Stamen;
RX PubMed=8220457; DOI=10.1046/j.1365-313x.1993.03040495.x;
RA de Oliveira D.E., Franco L.O., Simoens C., Seurinck J., Coppieters J.,
RA Botterman J., Van Montagu M.;
RT "Inflorescence-specific genes from Arabidopsis thaliana encoding glycine-
RT rich proteins.";
RL Plant J. 3:495-507(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 3; 4; 5 AND 6), SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Cvi-0, cv. Kas-1, cv. Landsberg erecta, and cv. Wassilewskija-2;
RX PubMed=11431566; DOI=10.1126/science.1060972;
RA Mayfield J.A., Fiebig A., Johnstone S.E., Preuss D.;
RT "Gene families from the Arabidopsis thaliana pollen coat proteome.";
RL Science 292:2482-2485(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=11929861; DOI=10.1074/jbc.m109298200;
RA Kim H.U., Hsieh K., Ratnayake C., Huang A.H.C.;
RT "A novel group of oleosins is present inside the pollen of Arabidopsis.";
RL J. Biol. Chem. 277:22677-22684(2002).
RN [7]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=14739246; DOI=10.1093/molbev/msh059;
RA Schein M., Yang Z., Mitchell-Olds T., Schmid K.J.;
RT "Rapid evolution of a pollen-specific oleosin-like gene family from
RT Arabidopsis thaliana and closely related species.";
RL Mol. Biol. Evol. 21:659-669(2004).
CC -!- FUNCTION: Lipid-binding oleosin involved in tapetum development,
CC especially for the physiology of tapetosomes (By similarity). Also
CC implicated in the formation of pollen coat (By similarity).
CC {ECO:0000250|UniProtKB:Q42574}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix, pollen coat {ECO:0000269|PubMed:11431566}. Lipid droplet
CC {ECO:0000250|UniProtKB:Q9LY09}. Membrane {ECO:0000255}; Multi-pass
CC membrane protein {ECO:0000255}. Note=Surface of oil bodies. Oleosins
CC exist at a monolayer lipid/water interface.
CC {ECO:0000250|UniProtKB:C3S7F0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=6;
CC Name=1;
CC IsoId=Q9LY08-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9LY08-2; Sequence=VSP_061405;
CC Name=3;
CC IsoId=Q9LY08-3; Sequence=VSP_061403, VSP_061407;
CC Name=4;
CC IsoId=Q9LY08-4; Sequence=VSP_061406;
CC Name=5;
CC IsoId=Q9LY08-5; Sequence=VSP_061404, VSP_061407;
CC Name=6;
CC IsoId=Q9LY08-6; Sequence=VSP_061402, VSP_061403, VSP_061407;
CC -!- TISSUE SPECIFICITY: Inflorescence-specific expression, especially in
CC buds and flowers florets (PubMed:8220457, PubMed:11929861,
CC PubMed:14739246). Present in pollen (at protein level)
CC (PubMed:11431566). {ECO:0000269|PubMed:11431566,
CC ECO:0000269|PubMed:11929861, ECO:0000269|PubMed:14739246,
CC ECO:0000269|PubMed:8220457}.
CC -!- DEVELOPMENTAL STAGE: In flowers, present in the tapetum.
CC {ECO:0000269|PubMed:11929861}.
CC -!- SIMILARITY: Belongs to the oleosin family. {ECO:0000305}.
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DR EMBL; Z11858; CAA77881.1; -; Genomic_DNA.
DR EMBL; Z11867; CAA77893.1; -; mRNA.
DR EMBL; AF362474; AAK83828.1; -; Genomic_DNA.
DR EMBL; AF362475; AAK83834.1; -; Genomic_DNA.
DR EMBL; AF362484; AAK83846.1; -; Genomic_DNA.
DR EMBL; AF362485; AAK83847.1; -; Genomic_DNA.
DR EMBL; AL163912; CAB87943.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91173.1; -; Genomic_DNA.
DR EMBL; BT003862; AAO41912.1; -; mRNA.
DR EMBL; BT005157; AAO50690.1; -; mRNA.
DR PIR; S19932; S19932.
DR PIR; T49893; T49893.
DR RefSeq; NP_196371.1; NM_120836.5.
DR AlphaFoldDB; Q9LY08; -.
DR STRING; 3702.AT5G07540.1; -.
DR PaxDb; Q9LY08; -.
DR PRIDE; Q9LY08; -.
DR EnsemblPlants; AT5G07540.1; AT5G07540.1; AT5G07540. [Q9LY08-1]
DR EnsemblPlants; AT5G07540.2; AT5G07540.2; AT5G07540.
DR GeneID; 830647; -.
DR Gramene; AT5G07540.1; AT5G07540.1; AT5G07540. [Q9LY08-1]
DR Gramene; AT5G07540.2; AT5G07540.2; AT5G07540.
DR KEGG; ath:AT5G07540; -.
DR Araport; AT5G07540; -.
DR TAIR; locus:2183399; AT5G07540.
DR HOGENOM; CLU_1139376_0_0_1; -.
DR InParanoid; Q9LY08; -.
DR OMA; GKRLAWW; -.
DR PhylomeDB; Q9LY08; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LY08; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0012511; C:monolayer-surrounded lipid storage body; IEA:InterPro.
DR GO; GO:0070505; C:pollen coat; IEA:UniProtKB-SubCell.
DR GO; GO:0008289; F:lipid binding; ISS:TAIR.
DR GO; GO:0019915; P:lipid storage; IBA:GO_Central.
DR GO; GO:0022414; P:reproductive process; IEA:UniProt.
DR GO; GO:0019953; P:sexual reproduction; IBA:GO_Central.
DR InterPro; IPR000136; Oleosin.
DR PANTHER; PTHR33203; PTHR33203; 1.
DR Pfam; PF01277; Oleosin; 1.
DR PROSITE; PS00811; OLEOSINS; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Extracellular matrix; Lipid droplet; Membrane;
KW Reference proteome; Repeat; Secreted; Transmembrane; Transmembrane helix.
FT CHAIN 1..244
FT /note="Tapetal oleosin GRP-16"
FT /id="PRO_0000454876"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 110..113
FT /note="A-1"
FT /evidence="ECO:0000255"
FT REPEAT 114..117
FT /note="A-2"
FT /evidence="ECO:0000255"
FT REPEAT 118..121
FT /note="B-1"
FT /evidence="ECO:0000255"
FT REPEAT 124..127
FT /note="A-3"
FT /evidence="ECO:0000255"
FT REPEAT 128..131
FT /note="A-4"
FT /evidence="ECO:0000255"
FT REPEAT 132..135
FT /note="B-2"
FT /evidence="ECO:0000255"
FT REPEAT 136..139
FT /note="A-5"
FT /evidence="ECO:0000255"
FT REPEAT 142..145
FT /note="B-3"
FT /evidence="ECO:0000255"
FT REPEAT 146..149
FT /note="A-6"
FT /evidence="ECO:0000255"
FT REPEAT 150..153
FT /note="A-7"
FT /evidence="ECO:0000255"
FT REPEAT 154..157
FT /note="A-8"
FT /evidence="ECO:0000255"
FT REPEAT 158..161
FT /note="A-9"
FT /evidence="ECO:0000255"
FT REPEAT 162..165
FT /note="A-10"
FT /evidence="ECO:0000255"
FT REPEAT 166..169
FT /note="A-11"
FT /evidence="ECO:0000255"
FT REPEAT 170..173
FT /note="A-12"
FT /evidence="ECO:0000255"
FT REPEAT 174..177
FT /note="A-13"
FT /evidence="ECO:0000255"
FT REPEAT 178..181
FT /note="A-14"
FT /evidence="ECO:0000255"
FT REPEAT 182..185
FT /note="A-15"
FT /evidence="ECO:0000255"
FT REPEAT 186..189
FT /note="A-16"
FT /evidence="ECO:0000255"
FT REPEAT 190..193
FT /note="A-17"
FT /evidence="ECO:0000255"
FT REPEAT 194..197
FT /note="A-18"
FT /evidence="ECO:0000255"
FT REPEAT 198..201
FT /note="A-19"
FT /evidence="ECO:0000255"
FT REPEAT 202..205
FT /note="A-20"
FT /evidence="ECO:0000255"
FT REPEAT 206..209
FT /note="B-4"
FT /evidence="ECO:0000255"
FT REPEAT 210..213
FT /note="A-21"
FT /evidence="ECO:0000255"
FT REPEAT 214..217
FT /note="B-5"
FT /evidence="ECO:0000255"
FT REPEAT 218..221
FT /note="A-22"
FT /evidence="ECO:0000255"
FT REPEAT 222..225
FT /note="B-6"
FT /evidence="ECO:0000255"
FT REPEAT 226..229
FT /note="B-7"
FT /evidence="ECO:0000255"
FT REPEAT 231..234
FT /note="B-8"
FT /evidence="ECO:0000255"
FT REPEAT 235..238
FT /note="A-23"
FT /evidence="ECO:0000255"
FT REGION 94..244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 110..238
FT /note="23 X 4 AA approximate repeat A"
FT REGION 118..234
FT /note="8 X 4 AA approximate repeat B"
FT VAR_SEQ 110..113
FT /note="Missing (in isoform 6)"
FT /id="VSP_061402"
FT VAR_SEQ 144..151
FT /note="Missing (in isoform 3 and isoform 6)"
FT /id="VSP_061403"
FT VAR_SEQ 145..164
FT /note="Missing (in isoform 5)"
FT /id="VSP_061404"
FT VAR_SEQ 159..180
FT /note="ASGGASGGASGGASGGASGGGP -> GPGGAS (in isoform 2)"
FT /id="VSP_061405"
FT VAR_SEQ 161..180
FT /note="Missing (in isoform 4)"
FT /id="VSP_061406"
FT VAR_SEQ 175..180
FT /note="ASGGGP -> GPGGAS (in isoform 3, isoform 5 and
FT isoform 6)"
FT /id="VSP_061407"
FT CONFLICT 25
FT /note="L -> F (in Ref. 2; AAK83847)"
FT /evidence="ECO:0000305"
FT CONFLICT 163..164
FT /note="AS -> GP (in Ref. 2; AAK83828/AAK83834)"
FT /evidence="ECO:0000305"
FT CONFLICT 220
FT /note="W -> S (in Ref. 1; CAA77881/CAA77893 and 2;
FT AAK83834/AAK83846/AAK83847/AAK83828)"
FT /evidence="ECO:0000305"
FT CONFLICT 233
FT /note="E -> A (in Ref. 1; CAA77881/CAA77893 and 2;
FT AAK83834/AAK83846/AAK83847/AAK83828)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 244 AA; 22300 MW; 109E19301304CCFF CRC64;
MLSFLIPVVQ FFQVVIAAVA SVVFLVFAGI TFGASIVGLT IATPLFVIFS PILVPATIAT
TFLVGGATAA VALGVTAFAL ILWLFKHRIG VKPKNNPAPK GAPTKADQPG ASGGASGDKP
GEMSGAGGPS GDKPGGASGG GDKPGGASGG GPGGASGGAS GGASGGASGG ASGGASGGGP
GGASGGGPGG ASGGGPGGAS GGASGDKPEG APGDKPGGAW GGKPGKKPGH KPEGARGGKR
LAWW
