ID   GRP2B_XENLA             Reviewed;         594 AA.
AC   Q6DCK3;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=RAS guanyl-releasing protein 2-B;
GN   Name=rasgrp2-b;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Spleen;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Functions as a calcium- and DAG-regulated nucleotide exchange
CC       factor specifically activating Rap through the exchange of bound GDP
CC       for GTP. May function in cell aggregation and adhesion.
CC       {ECO:0000250|UniProtKB:Q7LDG7}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}. Cell membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Synapse,
CC       synaptosome {ECO:0000250}. Note=Found both in the cytosol and
CC       associated with membranes. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RASGRP family. {ECO:0000305}.
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DR   EMBL; BC078012; AAH78012.1; -; mRNA.
DR   RefSeq; NP_001087119.1; NM_001093650.1.
DR   AlphaFoldDB; Q6DCK3; -.
DR   SMR; Q6DCK3; -.
DR   DNASU; 447008; -.
DR   GeneID; 447008; -.
DR   KEGG; xla:447008; -.
DR   CTD; 447008; -.
DR   Xenbase; XB-GENE-986629; rasgrp2.L.
DR   OrthoDB; 355412at2759; -.
DR   Proteomes; UP000186698; Chromosome 4L.
DR   Bgee; 447008; Expressed in spleen and 11 other tissues.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0043005; C:neuron projection; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0045202; C:synapse; IEA:UniProtKB-SubCell.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR   CDD; cd00029; C1; 1.
DR   CDD; cd00051; EFh; 1.
DR   CDD; cd00155; RasGEF; 1.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   InterPro; IPR046349; C1-like_sf.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   InterPro; IPR002219; PE/DAG-bd.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   PANTHER; PTHR23113; PTHR23113; 1.
DR   Pfam; PF00130; C1_1; 1.
DR   Pfam; PF00036; EF-hand_1; 1.
DR   Pfam; PF13202; EF-hand_5; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   SMART; SM00109; C1; 1.
DR   SMART; SM00054; EFh; 2.
DR   SMART; SM00147; RasGEF; 1.
DR   SMART; SM00229; RasGEFN; 1.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   SUPFAM; SSF57889; SSF57889; 1.
DR   PROSITE; PS00018; EF_HAND_1; 2.
DR   PROSITE; PS50222; EF_HAND_2; 2.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
DR   PROSITE; PS00479; ZF_DAG_PE_1; 1.
DR   PROSITE; PS50081; ZF_DAG_PE_2; 1.
PE   2: Evidence at transcript level;
KW   Calcium; Cell membrane; Cytoplasm; Guanine-nucleotide releasing factor;
KW   Membrane; Metal-binding; Reference proteome; Repeat; Synapse; Synaptosome;
KW   Zinc; Zinc-finger.
FT   CHAIN           1..594
FT                   /note="RAS guanyl-releasing protein 2-B"
FT                   /id="PRO_0000315612"
FT   DOMAIN          3..121
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT   DOMAIN          149..382
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT   DOMAIN          418..453
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          455..482
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   ZN_FING         490..540
FT                   /note="Phorbol-ester/DAG-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00226"
FT   REGION          377..403
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         431
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         433
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         435
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         437
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         442
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         460
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         462
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         464
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         466
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         471
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ   SEQUENCE   594 AA;  68338 MW;  8B6CC286623B8EBB CRC64;
     MDSSDLDKGL TIDEIIAKCI QSFDKDGKLS DPKLVQMFLM MHPWYIPSGD LAKKLFALSE
     SGDNVERERI CQFVRFWISE FPAEFDLNPE LGEQIRDLKR ALENKGNRRE SSLIDIESVP
     SYGWKRQVTQ RGPGGGRVRK TSLLFDHLDP AELAEHLTHL EFHSFSKILF QDYHSFVLHG
     CTVGNPVLER FIALFNGVSQ WIQLMVLSKH TPQQRAAVIK QFVQVAERLL QMQNFNTLMS
     VVGGLSHSSI SRLKDTQSHI SPETTKVYDS LLELLTSSDN YARYRRRFAT CKGFRFPALG
     VHLKDLMALH VALPDWADKA KTIINISKMR QVYKVVHELT EAQRLEPPVK ANPDLLNLLT
     VSLDQYRSEE EIYQLSLQRE PRARSTQTHA KSPPSPSPPL EEWASLKAKP DQALLCQHIE
     KMVESVFRLF DEDGDGHISQ EEFQSVRSNF PYLCAFNEID QNQDGKISKQ EMTSYFLRAS
     SVLDCKMGFI HNFAERTFLR PVSCQHCRNL ILGIYKKGLK CKACGITCHK HCRDHLSIEC
     KKRSKSVSER GESMEKGRHF FFTLPRSFRR STLYPDLREE EPHMEDDGVF DDHL