ID   GRP2_PINMA              Reviewed;         294 AA.
AC   P86969;
DT   21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT   21-SEP-2011, sequence version 1.
DT   03-AUG-2022, entry version 16.
DE   RecName: Full=Glycine-rich protein 2;
DE   AltName: Full=Nacre uncharacterized shell protein 6;
DE            Short=NUSP6;
DE   Flags: Precursor;
OS   Pinctada maxima (Silver-lipped pearl oyster) (White-lipped pearl oyster).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia;
OC   Autobranchia; Pteriomorphia; Pterioida; Pterioidea; Pteriidae; Pinctada.
OX   NCBI_TaxID=104660;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND IDENTIFICATION.
RC   TISSUE=Mantle {ECO:0000269|PubMed:19915030};
RX   PubMed=19915030; DOI=10.1093/molbev/msp278;
RA   Jackson D.J., McDougall C., Woodcroft B., Moase P., Rose R.A., Kube M.,
RA   Reinhardt R., Rokhsar D.S., Montagnani C., Joubert C., Piquemal D.,
RA   Degnan B.M.;
RT   "Parallel evolution of nacre building gene sets in molluscs.";
RL   Mol. Biol. Evol. 27:591-608(2010).
RN   [2]
RP   PROTEIN SEQUENCE OF 84-94; 133-153 AND 273-280, SUBCELLULAR LOCATION, AND
RP   TISSUE SPECIFICITY.
RC   TISSUE=Shell;
RX   PubMed=23213212; DOI=10.1073/pnas.1210552109;
RA   Marie B., Joubert C., Tayale A., Zanella-Cleon I., Belliard C.,
RA   Piquemal D., Cochennec-Laureau N., Marin F., Gueguen Y., Montagnani C.;
RT   "Different secretory repertoires control the biomineralization processes of
RT   prism and nacre deposition of the pearl oyster shell.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:20986-20991(2012).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23213212}.
CC   -!- TISSUE SPECIFICITY: Nacreous layer of shell (at protein level).
CC       Expressed primarily in the mantle with highest level in the mantle
CC       pallium and lower level in the mantle edge.
CC       {ECO:0000269|PubMed:23213212}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=GT277832; Type=Miscellaneous discrepancy; Note=Premature stop codon at position 283.; Evidence={ECO:0000305};
CC       Sequence=GT277939; Type=Miscellaneous discrepancy; Note=Premature stop codon at position 35.; Evidence={ECO:0000305};
CC       Sequence=GT278267; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=GT279323; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=GT282974; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=GT284326; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; GT277832; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT277939; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT278267; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT278413; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT278416; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT278464; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT279323; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT279413; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT279459; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT280703; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT281122; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT281315; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT281945; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT282886; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT282974; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT284148; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT284326; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT284381; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; EZ420153; -; NOT_ANNOTATED_CDS; mRNA.
DR   AlphaFoldDB; P86969; -.
DR   SMR; P86969; -.
DR   PRIDE; P86969; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..294
FT                   /note="Glycine-rich protein 2"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000412716"
FT   REGION          205..225
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        23
FT                   /note="G -> A (in Ref. 1; GT277939)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        34
FT                   /note="T -> F (in Ref. 1; GT277939)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        108
FT                   /note="G -> R (in Ref. 1; GT278464/GT284148/GT279323/
FT                   GT284326/GT280703/GT278267)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        146
FT                   /note="A -> P (in Ref. 1; GT278464)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        151
FT                   /note="D -> Y (in Ref. 1; GT284148)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        161..162
FT                   /note="KM -> LL (in Ref. 1; GT277832)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        167
FT                   /note="A -> S (in Ref. 1; GT278267)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        171..172
FT                   /note="GA -> VV (in Ref. 1; GT277832)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        182
FT                   /note="A -> P (in Ref. 1; GT278267)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        230
FT                   /note="R -> K (in Ref. 1; GT280703/GT281315)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   294 AA;  30054 MW;  6214004C439F2128 CRC64;
     MKMWFRLATF VTLIIEFAHC QLGAGLTSAL TGNTGNAAHA GNSGNMATSM AMAAAMGSDF
     AALGPLGAKL FSGAGMSPAV AYKMFDGSVA DFQKYKAKMN LMKRLNAGGS GGIAGGGGGG
     ATGGLGALAM GKLALFTGGG MDPVVAYKMF DGSVSDFQKY KMKKKLAAAA GAGGGMGIGT
     LAMMDGSMAD MAKFNMLSKV LSPPTGSQTG AAANGTSAGA AVRGGSSPLR RIANMRMTRK
     LCEDTPVVYR MRRCSPQLPC RHALTTCKQT LRYGSVCCAK NLYAAAMFEN MPGF