ID   GRP3S_ARATH             Reviewed;         116 AA.
AC   Q9ZSJ6; Q94A93; Q9SHT6;
DT   15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 2.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Glycine-rich protein 3 short isoform;
DE   Flags: Precursor;
GN   Name=GRP3S; OrderedLocusNames=At2g05380; ORFNames=F16J10.7;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH WAK1.
RX   PubMed=11335717; DOI=10.1074/jbc.m101283200;
RA   Park A.R., Cho S.K., Yun U.J., Jin M.Y., Lee S.H., Sachetto-Martins G.,
RA   Park O.K.;
RT   "Interaction of the Arabidopsis receptor protein kinase Wak1 with a
RT   glycine-rich protein, AtGRP-3.";
RL   J. Biol. Chem. 276:26688-26693(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
CC   -!- FUNCTION: Regulates the function of the receptor protein kinase WAK1.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with WAK1 (via the extracellular domain). Component
CC       of a 500 kDa complex, composed of GRP3 or GRP3-S, WAK1 and KAPP.
CC       {ECO:0000269|PubMed:11335717}.
CC   -!- INTERACTION:
CC       Q9ZSJ6; Q39191: WAK1; NbExp=2; IntAct=EBI-2319984, EBI-2320121;
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC       matrix {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q9ZSJ6-1; Sequence=Displayed;
CC   -!- MISCELLANEOUS: GRP3 and GRP3S bind to WAK1, WAK3 and WAK5, but GRP2,
CC       GRP4,GRP6, GRP7 and GRP8 did not bind to any of the WAK isoforms.
CC   -!- SIMILARITY: Belongs to the GRP family. {ECO:0000305}.
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DR   EMBL; AF104330; AAD11798.1; -; mRNA.
DR   EMBL; AC007289; AAD32899.2; -; Genomic_DNA.
DR   EMBL; CP002685; AEC05923.1; -; Genomic_DNA.
DR   EMBL; AY049267; AAK83609.1; -; mRNA.
DR   EMBL; BT001135; AAN64526.1; -; mRNA.
DR   PIR; G84467; G84467.
DR   RefSeq; NP_565325.1; NM_126561.4. [Q9ZSJ6-1]
DR   AlphaFoldDB; Q9ZSJ6; -.
DR   BioGRID; 486; 1.
DR   IntAct; Q9ZSJ6; 1.
DR   STRING; 3702.AT2G05380.1; -.
DR   PaxDb; Q9ZSJ6; -.
DR   PRIDE; Q9ZSJ6; -.
DR   ProteomicsDB; 230055; -. [Q9ZSJ6-1]
DR   DNASU; 815086; -.
DR   EnsemblPlants; AT2G05380.1; AT2G05380.1; AT2G05380. [Q9ZSJ6-1]
DR   GeneID; 815086; -.
DR   Gramene; AT2G05380.1; AT2G05380.1; AT2G05380. [Q9ZSJ6-1]
DR   KEGG; ath:AT2G05380; -.
DR   Araport; AT2G05380; -.
DR   TAIR; locus:2042803; AT2G05380.
DR   eggNOG; ENOG502S5AC; Eukaryota.
DR   HOGENOM; CLU_105596_2_0_1; -.
DR   InParanoid; Q9ZSJ6; -.
DR   PhylomeDB; Q9ZSJ6; -.
DR   PRO; PR:Q9ZSJ6; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q9ZSJ6; baseline and differential.
DR   Genevisible; Q9ZSJ6; AT.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR   InterPro; IPR010800; GRP.
DR   PANTHER; PTHR37389; PTHR37389; 1.
DR   Pfam; PF07172; GRP; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Extracellular matrix; Reference proteome; Repeat;
KW   Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..116
FT                   /note="Glycine-rich protein 3 short isoform"
FT                   /id="PRO_0000389635"
FT   REPEAT          54..59
FT                   /note="1"
FT   REPEAT          62..67
FT                   /note="2"
FT   REPEAT          68..73
FT                   /note="3"
FT   REPEAT          75..80
FT                   /note="4"
FT   REPEAT          81..86
FT                   /note="5"
FT   REGION          27..83
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          54..86
FT                   /note="5 X 6 AA tandem repeats of G-G-G-G-[HYRS]-[GYQ]"
FT   CONFLICT        5
FT                   /note="T -> A (in Ref. 1; AAD11798)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        114
FT                   /note="S -> P (in Ref. 1; AAD11798)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   116 AA;  11586 MW;  A0E8A065AAB8433C CRC64;
     MASKTLLLLG LFAFLFIVSE MAAAGTVKSE SEETVKPEQH GGGFGDNGGG RYQGGGGHGG
     HGGGGYQGGG GRYQGGGGRQ GGGGSYCRHG CCYKGYHGCS RCCSYAGEAV QTQSGH