ID   AMPD2_STEME             Reviewed;          81 AA.
AC   C0HL83;
DT   28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT   28-MAR-2018, sequence version 1.
DT   25-MAY-2022, entry version 6.
DE   RecName: Full=Antimicrobial peptide D2 {ECO:0000303|PubMed:21056078};
DE            Short=Sm-AMP-D2 {ECO:0000303|PubMed:21056078};
DE            Short=Sm-D2 {ECO:0000303|PubMed:21056078};
DE   Flags: Precursor;
OS   Stellaria media (Common chickweed) (Alsine media).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Caryophyllaceae; Alsineae; Stellaria.
OX   NCBI_TaxID=13274 {ECO:0000303|PubMed:21056078};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 32-81,
RP   FUNCTION, MASS SPECTROMETRY, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Seed {ECO:0000303|PubMed:21056078};
RX   PubMed=21056078; DOI=10.1016/j.biochi.2010.10.019;
RA   Slavokhotova A.A., Odintsova T.I., Rogozhin E.A., Musolyamov A.K.,
RA   Andreev Y.A., Grishin E.V., Egorov T.A.;
RT   "Isolation, molecular cloning and antimicrobial activity of novel defensins
RT   from common chickweed (Stellaria media L.) seeds.";
RL   Biochimie 93:450-456(2011).
CC   -!- FUNCTION: Antimicrobial peptide probably active against fungi like
CC       B.sorokiniana, F.oxysporum, F.graminearum, F.avenaceum, B.cinerea,
CC       P.beta, P.infestans and P.debaryanum. {ECO:0000269|PubMed:21056078}.
CC   -!- MASS SPECTROMETRY: Mass=5755.5; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:21056078};
CC   -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; C0HL83; -.
DR   SMR; C0HL83; -.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR008176; Defensin_plant.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS00940; GAMMA_THIONIN; 1.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Disulfide bond; Fungicide;
KW   Signal.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000269|PubMed:21056078"
FT   PEPTIDE         32..81
FT                   /note="Antimicrobial peptide D2"
FT                   /evidence="ECO:0000269|PubMed:21056078"
FT                   /id="PRO_0000443560"
FT   DISULFID        34..81
FT                   /evidence="ECO:0000250|UniProtKB:P69241"
FT   DISULFID        45..66
FT                   /evidence="ECO:0000250|UniProtKB:P69241"
FT   DISULFID        51..75
FT                   /evidence="ECO:0000250|UniProtKB:P69241"
FT   DISULFID        55..77
FT                   /evidence="ECO:0000250|UniProtKB:P69241"
SQ   SEQUENCE   81 AA;  9208 MW;  767AA99F46956A71 CRC64;
     MAKTVLGIHV TFLTLLFAVI LLNDVMYTPV EKICERASGT WKGICIHSND CNNQCVKWEN
     AGSGSCHYQF PNYMCFCYFN C