ID   GRPE1_BOVIN             Reviewed;         217 AA.
AC   Q3SZC1; Q9T2U7;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=GrpE protein homolog 1, mitochondrial;
DE   AltName: Full=Mt-GrpE#1;
DE            Short=mt-GrpE;
DE   Flags: Precursor;
GN   Name=GRPEL1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 28-55 AND 170-196.
RC   TISSUE=Liver;
RX   PubMed=7711059; DOI=10.1016/0167-4838(95)00007-h;
RA   Naylor D.J., Ryan M.T., Condron R., Hoogenraad N.J., Hoj P.B.;
RT   "Affinity-purification and identification of GrpE homologues from mammalian
RT   mitochondria.";
RL   Biochim. Biophys. Acta 1248:75-79(1995).
CC   -!- FUNCTION: Essential component of the PAM complex, a complex required
CC       for the translocation of transit peptide-containing proteins from the
CC       inner membrane into the mitochondrial matrix in an ATP-dependent
CC       manner. Seems to control the nucleotide-dependent binding of
CC       mitochondrial HSP70 to substrate proteins (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Probable component of the PAM complex at least composed of a
CC       mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and
CC       TIMM14/DNAJC19. Binds to HSP70, HSC70 and HSJ1B (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC   -!- SIMILARITY: Belongs to the GrpE family. {ECO:0000305}.
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DR   EMBL; BC102964; AAI02965.1; -; mRNA.
DR   RefSeq; NP_001029673.1; NM_001034501.2.
DR   AlphaFoldDB; Q3SZC1; -.
DR   SMR; Q3SZC1; -.
DR   STRING; 9913.ENSBTAP00000013431; -.
DR   PaxDb; Q3SZC1; -.
DR   PeptideAtlas; Q3SZC1; -.
DR   PRIDE; Q3SZC1; -.
DR   Ensembl; ENSBTAT00000013431; ENSBTAP00000013431; ENSBTAG00000010181.
DR   GeneID; 515580; -.
DR   KEGG; bta:515580; -.
DR   CTD; 80273; -.
DR   VEuPathDB; HostDB:ENSBTAG00000010181; -.
DR   VGNC; VGNC:29665; GRPEL1.
DR   eggNOG; KOG3003; Eukaryota.
DR   GeneTree; ENSGT00390000005589; -.
DR   HOGENOM; CLU_057217_0_1_1; -.
DR   InParanoid; Q3SZC1; -.
DR   OMA; YAYEKIA; -.
DR   OrthoDB; 1525208at2759; -.
DR   TreeFam; TF105284; -.
DR   Proteomes; UP000009136; Chromosome 6.
DR   Bgee; ENSBTAG00000010181; Expressed in oocyte and 105 other tissues.
DR   ExpressionAtlas; Q3SZC1; baseline and differential.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0001405; C:PAM complex, Tim23 associated import motor; IBA:GO_Central.
DR   GO; GO:0000774; F:adenyl-nucleotide exchange factor activity; IBA:GO_Central.
DR   GO; GO:0051087; F:chaperone binding; IEA:InterPro.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IBA:GO_Central.
DR   GO; GO:0006457; P:protein folding; IEA:InterPro.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   CDD; cd00446; GrpE; 1.
DR   Gene3D; 2.30.22.10; -; 1.
DR   Gene3D; 3.90.20.20; -; 1.
DR   HAMAP; MF_01151; GrpE; 1.
DR   InterPro; IPR000740; GrpE.
DR   InterPro; IPR013805; GrpE_coiled_coil.
DR   InterPro; IPR009012; GrpE_head.
DR   PANTHER; PTHR21237; PTHR21237; 1.
DR   Pfam; PF01025; GrpE; 1.
DR   PRINTS; PR00773; GRPEPROTEIN.
DR   SUPFAM; SSF51064; SSF51064; 1.
DR   SUPFAM; SSF58014; SSF58014; 1.
DR   PROSITE; PS01071; GRPE; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Chaperone; Direct protein sequencing; Mitochondrion;
KW   Reference proteome; Transit peptide.
FT   TRANSIT         1..27
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000269|PubMed:7711059"
FT   CHAIN           28..217
FT                   /note="GrpE protein homolog 1, mitochondrial"
FT                   /id="PRO_0000245345"
FT   MOD_RES         94
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q99LP6"
FT   MOD_RES         94
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q99LP6"
FT   MOD_RES         100
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q99LP6"
FT   MOD_RES         120
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q99LP6"
FT   MOD_RES         215
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q9HAV7"
FT   MOD_RES         215
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q99LP6"
FT   CONFLICT        28
FT                   /note="C -> S (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        36
FT                   /note="N -> D (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   217 AA;  24306 MW;  4BC6FB43FC049FF5 CRC64;
     MAARCVRLAR GSLPAFALSL RSSPRLLCTA AKQKNNGQNL EEDAGQNEQK TDLPSTEKTL
     MEEKVKLEEQ LKETMEKYKR ALADTENLRQ RSQKLVEEAK LYGIQGFCKD LLEVADILEK
     ATQCVPQEEI RDDNPHLKSL YEGLVMTEVQ IQKVFTKHGL LRLNPLGAKF DPYEHEALFH
     TPVEGKEPGT VALVNKVGYK LHGRTLRPAL VGVVKGA