ID   3SA1F_NAJAT             Reviewed;          15 AA.
AC   P85429;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 1.
DT   29-SEP-2021, entry version 26.
DE   RecName: Full=Cytotoxin 1f;
DE   AltName: Full=Cardiotoxin 1f;
DE   Flags: Fragment;
OS   Naja atra (Chinese cobra).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8656;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND TOXIC DOSE.
RC   TISSUE=Venom;
RX   PubMed=18438973; DOI=10.1002/jmr.876;
RA   Dong D., Liu H., Xiao Q., Wang T., Liu H., Li R.;
RT   "Biomimetic affinity purification of cardiotoxin and its pharmacological
RT   effects on the nervous system.";
RL   J. Mol. Recognit. 21:148-153(2008).
CC   -!- FUNCTION: Shows cytolytic activity on many different cells by forming
CC       pore in lipid membranes. In vivo, increases heart rate or kills the
CC       animal by cardiac arrest. In addition, it binds to heparin with high
CC       affinity, interacts with Kv channel-interacting protein 1 (KCNIP1) in a
CC       calcium-independent manner, and binds to integrin alpha-V/beta-3
CC       (ITGAV/ITGB3) with moderate affinity. {ECO:0000250|UniProtKB:P60301,
CC       ECO:0000250|UniProtKB:P60304}.
CC   -!- SUBUNIT: Monomer in solution; Homodimer and oligomer in the presence of
CC       negatively charged lipids forming a pore with a size ranging between 20
CC       and 30 Angstroms. {ECO:0000250|UniProtKB:P60301}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18438973}. Target
CC       cell membrane {ECO:0000250|UniProtKB:P60301}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=6800; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:18438973};
CC   -!- TOXIC DOSE: LD(50) is 2.17 mg/kg by intraperitoneal injection in mice.
CC       {ECO:0000269|PubMed:18438973}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Type IA cytotoxin sub-subfamily. {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cardiotoxin; Cytolysis; Direct protein sequencing; Disulfide bond;
KW   Membrane; Secreted; Target cell membrane; Target membrane; Toxin.
FT   CHAIN           1..>15
FT                   /note="Cytotoxin 1f"
FT                   /evidence="ECO:0000269|PubMed:18438973"
FT                   /id="PRO_0000320216"
FT   DISULFID        3..?
FT                   /evidence="ECO:0000250|UniProtKB:P01442"
FT   DISULFID        14..?
FT                   /evidence="ECO:0000250|UniProtKB:P01442"
FT   NON_TER         15
SQ   SEQUENCE   15 AA;  1767 MW;  35BE85588F8E3E4B CRC64;
     LKCNKLVPLF YKTCP