ID   AMPE_ECOLI              Reviewed;         284 AA.
AC   P0AE14; P13017;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Protein AmpE;
GN   Name=ampE; OrderedLocusNames=b0111, JW0107;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=2691840; DOI=10.1111/j.1365-2958.1989.tb00259.x;
RA   Lindquist S., Galleni M., Lindberg F., Normark S.;
RT   "Signalling proteins in enterobacterial AmpC beta-lactamase regulation.";
RL   Mol. Microbiol. 3:1091-1102(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=2607970; DOI=10.1111/j.1365-2958.1989.tb00262.x;
RA   Honore N., Nicolas M.H., Cole S.T.;
RT   "Regulation of enterobacterial cephalosporinase production: the role of a
RT   membrane-bound sensory transducer.";
RL   Mol. Microbiol. 3:1121-1130(1989).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: Putative signaling protein in beta-lactamase regulation. AmpE
CC       seems not to act as a direct sensor for beta-lactams.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   -!- INDUCTION: The level of AmpE affects the basal level of beta-lactamase
CC       expression in the absence of AmpD and in the presence of the AmpR
CC       transcriptional regulator.
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DR   EMBL; X15237; CAA33315.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73222.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAB96680.1; -; Genomic_DNA.
DR   PIR; S05570; S05570.
DR   RefSeq; NP_414653.1; NC_000913.3.
DR   RefSeq; WP_000172005.1; NZ_SSZK01000004.1.
DR   AlphaFoldDB; P0AE14; -.
DR   BioGRID; 4259732; 6.
DR   BioGRID; 851020; 1.
DR   DIP; DIP-47964N; -.
DR   IntAct; P0AE14; 2.
DR   STRING; 511145.b0111; -.
DR   TCDB; 9.B.125.1.1; the ampe/cobd (ampe/cobd) family.
DR   jPOST; P0AE14; -.
DR   PaxDb; P0AE14; -.
DR   PRIDE; P0AE14; -.
DR   EnsemblBacteria; AAC73222; AAC73222; b0111.
DR   EnsemblBacteria; BAB96680; BAB96680; BAB96680.
DR   GeneID; 58392247; -.
DR   GeneID; 946678; -.
DR   KEGG; ecj:JW0107; -.
DR   KEGG; eco:b0111; -.
DR   PATRIC; fig|1411691.4.peg.2171; -.
DR   EchoBASE; EB0040; -.
DR   eggNOG; COG3725; Bacteria.
DR   HOGENOM; CLU_054212_2_2_6; -.
DR   OMA; GPFRDAW; -.
DR   PhylomeDB; P0AE14; -.
DR   BioCyc; EcoCyc:EG10042-MON; -.
DR   PRO; PR:P0AE14; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0046677; P:response to antibiotic; IBA:GO_Central.
DR   InterPro; IPR031347; AmpE.
DR   Pfam; PF17113; AmpE; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..284
FT                   /note="Protein AmpE"
FT                   /id="PRO_0000064592"
FT   TOPO_DOM        1..43
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        61..67
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        85..194
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        195..211
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        212..267
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        268..284
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   284 AA;  32122 MW;  C92C05B2DFE0D6AD CRC64;
     MTLFTTLLVL IFERLFKLGE HWQLDHRLEA FFRRVKHFSL GRTLGMTIIA MGVTFLLLRA
     LQGVLFNVPT LLVWLLIGLL CIGAGKVRLH YHAYLTAASR NDSHARATMA GELTMIHGVP
     AGCDEREYLR ELQNALLWIN FRFYLAPLFW LIVGGTWGPV TLMGYAFLRA WQYWLARYQT
     PHHRLQSGID AVLHVLDWVP VRLAGVVYAL IGHGEKALPA WFASLGDFHT SQYQVLTRLA
     QFSLAREPHV DKVETPKAAV SMAKKTSFVV VVVIALLTIY GALV