ID   GRPE_DICDI              Reviewed;         213 AA.
AC   Q54QF9;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=GrpE protein homolog, mitochondrial;
DE   AltName: Full=dRoe1;
DE   Flags: Precursor;
GN   Name=grpE; ORFNames=DDB_G0283763;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Essential component of the PAM complex, a complex required
CC       for the translocation of transit peptide-containing proteins from the
CC       inner membrane into the mitochondrial matrix in an ATP-dependent
CC       manner. Seems to control the nucleotide-dependent binding of
CC       mitochondrial HSP70 to substrate proteins (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Probable component of the PAM complex at least composed of a
CC       mitochondrial HSP70 protein, grepE, tim16 and tim14. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the GrpE family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AAFI02000057; EAL65496.1; -; Genomic_DNA.
DR   RefSeq; XP_638912.1; XM_633820.1.
DR   AlphaFoldDB; Q54QF9; -.
DR   SMR; Q54QF9; -.
DR   STRING; 44689.DDB0216255; -.
DR   PaxDb; Q54QF9; -.
DR   EnsemblProtists; EAL65496; EAL65496; DDB_G0283763.
DR   GeneID; 8624309; -.
DR   KEGG; ddi:DDB_G0283763; -.
DR   dictyBase; DDB_G0283763; grpE.
DR   eggNOG; KOG3003; Eukaryota.
DR   HOGENOM; CLU_057217_0_1_1; -.
DR   InParanoid; Q54QF9; -.
DR   OMA; YAYEKIA; -.
DR   PhylomeDB; Q54QF9; -.
DR   PRO; PR:Q54QF9; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0005759; C:mitochondrial matrix; ISS:dictyBase.
DR   GO; GO:0005739; C:mitochondrion; ISS:dictyBase.
DR   GO; GO:0001405; C:PAM complex, Tim23 associated import motor; ISS:dictyBase.
DR   GO; GO:0000774; F:adenyl-nucleotide exchange factor activity; ISS:dictyBase.
DR   GO; GO:0051087; F:chaperone binding; IEA:InterPro.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IBA:GO_Central.
DR   GO; GO:0006457; P:protein folding; IEA:InterPro.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; ISS:dictyBase.
DR   CDD; cd00446; GrpE; 1.
DR   Gene3D; 2.30.22.10; -; 1.
DR   Gene3D; 3.90.20.20; -; 1.
DR   HAMAP; MF_01151; GrpE; 1.
DR   InterPro; IPR000740; GrpE.
DR   InterPro; IPR013805; GrpE_coiled_coil.
DR   InterPro; IPR009012; GrpE_head.
DR   PANTHER; PTHR21237; PTHR21237; 1.
DR   Pfam; PF01025; GrpE; 1.
DR   PRINTS; PR00773; GRPEPROTEIN.
DR   SUPFAM; SSF51064; SSF51064; 1.
DR   SUPFAM; SSF58014; SSF58014; 1.
PE   3: Inferred from homology;
KW   Chaperone; Mitochondrion; Reference proteome; Transit peptide.
FT   TRANSIT         1..32
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           33..213
FT                   /note="GrpE protein homolog, mitochondrial"
FT                   /id="PRO_0000328895"
FT   REGION          34..54
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        40..54
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   213 AA;  24585 MW;  D94EE7ADCA4C8CA6 CRC64;
     MNSLIRRGLV SIRTSTVIKP SSFGLMRNNR FYSTENNQEA AKPEETENKP APGSLEETIE
     KLKEELEETK KQLLYTAADR ENVRRFAKED NEKAKKFGIQ SFTKELLEVV DQLEMATNLF
     PKEKLDENKE LKDLHEGVKM TEQLFLKIMG NQGLQRFNPI GEKFDFNNHH AIFELNDPTK
     ENNTVGHVVK QGYKLHDRLV RPAMVGVNKI KPQ