ID   AMPI_ANTMY              Reviewed;          20 AA.
AC   P84885;
DT   06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   25-MAY-2022, entry version 13.
DE   RecName: Full=Protease inhibitor;
DE            Short=AmPI;
DE   Flags: Fragment;
OS   Antheraea mylitta (Tasar silkworm).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC   Saturniidae; Saturniinae; Saturniini; Antheraea.
OX   NCBI_TaxID=34739;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL
RP   STAGE, AND GLYCOSYLATION.
RC   STRAIN=Daba bivoltine {ECO:0000269|Ref.1};
RC   TISSUE=Hemolymph {ECO:0000269|Ref.1};
RA   Rai S.;
RT   "Ecological and biochemical studies on Terminalia arjuna - Antheraea
RT   mylitta herbivory.";
RL   Thesis (2006), Indraprastha University, India.
RN   [2]
RP   PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE
RP   SPECIFICITY, DEVELOPMENTAL STAGE, AND GLYCOSYLATION.
RC   STRAIN=Daba bivoltine; TISSUE=Larval hemolymph;
RX   PubMed=19723549; DOI=10.1016/j.peptides.2009.08.021;
RA   Rai S., Aggarwal K.K., Mitra B., Das T.K., Babu C.R.;
RT   "Purification, characterization and immunolocalization of a novel protease
RT   inhibitor from hemolymph of tasar silkworm, Antheraea mylitta.";
RL   Peptides 31:474-481(2010).
CC   -!- FUNCTION: Inhibits trypsin and chymotrypsin.
CC       {ECO:0000269|PubMed:19723549, ECO:0000269|Ref.1}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 4.5-9.0. High activity is also seen over the range pH
CC         2.0-4.5. Activity decreases with increasing pH above pH 9.0.
CC         {ECO:0000269|PubMed:19723549};
CC       Temperature dependence:
CC         Optimum temperature is 4-65 degrees Celsius. Activity decreases
CC         rapidly at higher temperatures, nearly 40% of activity is retained at
CC         100 degrees Celsius. {ECO:0000269|PubMed:19723549};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:19723549, ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Stored in epidermis and secreted into the hemolymph
CC       and cuticle. Not detected in the interior of the epidermis, fat body
CC       cells or columnar or goblet cells of the midgut epithelium (at protein
CC       level). {ECO:0000269|PubMed:19723549, ECO:0000269|Ref.1}.
CC   -!- DEVELOPMENTAL STAGE: Present in newly hatched larvae. Strongly
CC       expressed in the cuticle of fourth and fifth instar larvae. In
CC       epidermis, levels decreased with the increase in age of instars. In
CC       cuticle, levels increased with increase in age of instars.
CC       {ECO:0000269|PubMed:19723549, ECO:0000269|Ref.1}.
CC   -!- PTM: Glycosylated. {ECO:0000269|PubMed:19723549, ECO:0000269|Ref.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P84885; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycoprotein; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..>20
FT                   /note="Protease inhibitor"
FT                   /id="PRO_0000279692"
FT   NON_TER         20
FT                   /evidence="ECO:0000303|PubMed:19723549, ECO:0000303|Ref.1"
SQ   SEQUENCE   20 AA;  2052 MW;  BAC4DD3736306BF2 CRC64;
     AEPLASQLKE PIAGGGWWAA