ID   GRP_BOMOR               Reviewed;         155 AA.
AC   P29007;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Gastrin-releasing peptide;
DE            Short=GRP-29;
DE   Contains:
DE     RecName: Full=Neuromedin-C;
DE     AltName: Full=GRP-10;
DE   Contains:
DE     RecName: Full=C-terminal extension peptide;
DE              Short=CTEP;
DE   Flags: Precursor;
GN   Name=grp;
OS   Bombina orientalis (Oriental fire-bellied toad).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Bombinatoridae; Bombina.
OX   NCBI_TaxID=8346;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 51-60, AMIDATION AT MET-60,
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION OF
RP   GASTRIN-RELEASING PEPTIDE.
RC   TISSUE=Intestine;
RX   PubMed=1551901; DOI=10.1016/s0021-9258(19)50516-6;
RA   Nagalla S.R., Gibson B.W., Tang D., Reeve J.R. Jr., Spindel E.R.;
RT   "Gastrin-releasing peptide (GRP) is not mammalian bombesin. Identification
RT   and molecular cloning of a true amphibian GRP distinct from amphibian
RT   bombesin in Bombina orientalis.";
RL   J. Biol. Chem. 267:6916-6922(1992).
CC   -!- FUNCTION: Stimulates the release of gastrin and other gastrointestinal
CC       hormones. {ECO:0000250|UniProtKB:P63153}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P07492}.
CC       Cytoplasmic vesicle, secretory vesicle lumen
CC       {ECO:0000250|UniProtKB:Q863C3}.
CC   -!- TISSUE SPECIFICITY: Brain and stomach. In the stomach GRP was
CC       localized, at the base of the gastric pits, to occasional cells whose
CC       distribution and appearance were consistent with that of gut
CC       neuroendocrine cells.
CC   -!- SIMILARITY: Belongs to the bombesin/neuromedin-B/ranatensin family.
CC       {ECO:0000305}.
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DR   EMBL; M83737; AAA51409.1; -; mRNA.
DR   PIR; A42437; A42437.
DR   AlphaFoldDB; P29007; -.
DR   GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR   GO; GO:0034774; C:secretory granule lumen; ISS:UniProtKB.
DR   GO; GO:0005184; F:neuropeptide hormone activity; IEA:InterPro.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:InterPro.
DR   GO; GO:0090277; P:positive regulation of peptide hormone secretion; ISS:UniProtKB.
DR   GO; GO:1900738; P:positive regulation of phospholipase C-activating G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR   InterPro; IPR000874; Bombesin.
DR   InterPro; IPR015674; Gastrin-RP.
DR   PANTHER; PTHR16866; PTHR16866; 1.
DR   PANTHER; PTHR16866:SF2; PTHR16866:SF2; 1.
DR   Pfam; PF02044; Bombesin; 1.
DR   PROSITE; PS00257; BOMBESIN; 1.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Cytoplasmic vesicle;
KW   Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000269|PubMed:1551901"
FT   PEPTIDE         32..60
FT                   /note="Gastrin-releasing peptide"
FT                   /evidence="ECO:0000269|PubMed:1551901"
FT                   /id="PRO_0000003047"
FT   PEPTIDE         51..60
FT                   /note="Neuromedin-C"
FT                   /evidence="ECO:0000269|PubMed:1551901"
FT                   /id="PRO_0000003048"
FT   PEPTIDE         64..125
FT                   /note="C-terminal extension peptide"
FT                   /evidence="ECO:0000269|PubMed:1551901"
FT                   /id="PRO_0000003049"
FT   PROPEP          128..155
FT                   /id="PRO_0000003050"
FT   MOD_RES         60
FT                   /note="Methionine amide"
FT                   /evidence="ECO:0000269|PubMed:1551901"
SQ   SEQUENCE   155 AA;  17813 MW;  8972A6EE05AC474F CRC64;
     MEGVLLFWKY RALFFLVLCS LVLCKVHLSQ ASPTSQQHND AASLSKIYPR GSHWAVGHLM
     GKKSIEEYPY AYDEADRSSA AVFSEGDKPS DGYQQWKESL LNLLKMIEVN EYRNSKAMRE
     ASVYNKKFSG AEDNNLKEML DYLYQMMNMK ENTSS