GRP_SHEEP
ID GRP_SHEEP Reviewed; 134 AA.
AC P47851;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Gastrin-releasing peptide;
DE Short=GRP;
DE Contains:
DE RecName: Full=Neuromedin-C;
DE AltName: Full=GRP-10;
DE AltName: Full=GRP18-27 {ECO:0000250|UniProtKB:Q8R1I2};
DE Flags: Precursor;
GN Name=GRP;
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7988429; DOI=10.1210/endo.135.6.7988429;
RA Fraser M., McDonald T.J., Spindel E.R., Fahy M., Hill D., Challis J.R.;
RT "Gastrin-releasing peptide is produced in the pregnant ovine uterus.";
RL Endocrinology 135:2440-2445(1994).
CC -!- FUNCTION: Stimulates the release of gastrin and other gastrointestinal
CC hormones (By similarity). Contributes to the perception of prurient
CC stimuli and to the transmission of itch signals in the spinal cord that
CC promote scratching behavior (By similarity). Contributes primarily to
CC nonhistaminergic itch sensation (By similarity). In one study, shown to
CC act in the amygdala as part of an inhibitory network which inhibits
CC memory specifically related to learned fear (By similarity). In another
CC study, shown to act on vasoactive intestinal peptide (VIP)-expressing
CC cells in the auditory cortex, most likely via extrasynaptic diffusion
CC from local and long-range sources, to mediate disinhibition of
CC glutamatergic cells via VIP cell-specific GRPR signaling which leads to
CC enhanced auditory fear memories (By similarity). Contributes to the
CC regulation of food intake (By similarity). Inhibits voltage-gated
CC sodium channels but enhances voltage-gated potassium channels in
CC hippocampal neurons (By similarity). Induces sighing by acting directly
CC on the pre-Botzinger complex, a cluster of several thousand neurons in
CC the ventrolateral medulla responsible for inspiration during
CC respiratory activity (By similarity). {ECO:0000250|UniProtKB:P24393,
CC ECO:0000250|UniProtKB:P63153, ECO:0000250|UniProtKB:Q8R1I2}.
CC -!- FUNCTION: [Neuromedin-C]: Induces an itch response through activation
CC of receptors present on mast cells, triggering mast cell degranulation.
CC {ECO:0000250|UniProtKB:Q8R1I2}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P07492}.
CC Cytoplasmic vesicle, secretory vesicle lumen
CC {ECO:0000250|UniProtKB:Q863C3}. Cell projection, neuron projection
CC {ECO:0000250|UniProtKB:Q8R1I2}. Note=In neurons of the retrotrapezoid
CC nucleus/parafacial respiratory group, expressed on neuron projections
CC which project into the pre-Botzinger complex.
CC {ECO:0000250|UniProtKB:Q8R1I2}.
CC -!- SIMILARITY: Belongs to the bombesin/neuromedin-B/ranatensin family.
CC {ECO:0000305}.
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DR EMBL; S75723; AAB32675.1; -; mRNA.
DR PIR; I47010; I47010.
DR RefSeq; NP_001009321.1; NM_001009321.1.
DR AlphaFoldDB; P47851; -.
DR STRING; 9940.ENSOARP00000004291; -.
DR Ensembl; ENSOART00020039199; ENSOARP00020032472; ENSOARG00020024998.
DR GeneID; 443332; -.
DR KEGG; oas:443332; -.
DR CTD; 2922; -.
DR eggNOG; ENOG502TKSH; Eukaryota.
DR OrthoDB; 1427338at2759; -.
DR Proteomes; UP000002356; Unplaced.
DR GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR GO; GO:0043005; C:neuron projection; ISS:UniProtKB.
DR GO; GO:0034774; C:secretory granule lumen; ISS:UniProtKB.
DR GO; GO:0005184; F:neuropeptide hormone activity; IEA:InterPro.
DR GO; GO:0043303; P:mast cell degranulation; ISS:UniProtKB.
DR GO; GO:1903817; P:negative regulation of voltage-gated potassium channel activity; ISS:UniProtKB.
DR GO; GO:1905151; P:negative regulation of voltage-gated sodium channel activity; ISS:UniProtKB.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:InterPro.
DR GO; GO:2000987; P:positive regulation of behavioral fear response; ISS:UniProtKB.
DR GO; GO:0090277; P:positive regulation of peptide hormone secretion; ISS:UniProtKB.
DR GO; GO:1900738; P:positive regulation of phospholipase C-activating G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:1903942; P:positive regulation of respiratory gaseous exchange; ISS:UniProtKB.
DR InterPro; IPR000874; Bombesin.
DR InterPro; IPR015674; Gastrin-RP.
DR PANTHER; PTHR16866; PTHR16866; 1.
DR PANTHER; PTHR16866:SF2; PTHR16866:SF2; 1.
DR Pfam; PF02044; Bombesin; 1.
DR PROSITE; PS00257; BOMBESIN; 1.
PE 2: Evidence at transcript level;
KW Amidation; Cell projection; Cleavage on pair of basic residues;
KW Cytoplasmic vesicle; Mast cell degranulation; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000250|UniProtKB:P08989"
FT PEPTIDE 24..50
FT /note="Gastrin-releasing peptide"
FT /evidence="ECO:0000250|UniProtKB:P08989"
FT /id="PRO_0000003042"
FT PEPTIDE 41..50
FT /note="Neuromedin-C"
FT /evidence="ECO:0000250|UniProtKB:Q863C3"
FT /id="PRO_0000003043"
FT PROPEP 54..134
FT /evidence="ECO:0000250|UniProtKB:P08989"
FT /id="PRO_0000003044"
FT REGION 98..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 111..134
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 50
FT /note="Methionine amide"
FT /evidence="ECO:0000250|UniProtKB:P63153"
SQ SEQUENCE 134 AA; 14855 MW; C8958538E4C6F0A4 CRC64;
MRSREVSLVL LALVLCPAPR GSAAPVTAGR AGALAKMYTR GNHWAVGHLM GKKSVAESPQ
LREEESLKEQ LREYAQWEEA TRNLLSLLQA KVAQGHQPPR WEPLSIHQPA WDSKDVSNFK
DSGSQREGGN PQLY
