ID   GRST_ANEMI              Reviewed;         256 AA.
AC   P14686;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Gramicidin S biosynthesis protein GrsT;
DE            EC=3.1.2.-;
GN   Name=grsT;
OS   Aneurinibacillus migulanus (Bacillus migulanus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae;
OC   Aneurinibacillus group; Aneurinibacillus.
OX   NCBI_TaxID=47500;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 9999 / DSM 2895 / JCM 8504 / NBRC 15520 / NCIMB 7096 / NCTC
RC   7096;
RX   PubMed=2477357; DOI=10.1128/jb.171.10.5422-5429.1989;
RA   Kraetzschmar J., Krause M., Marahiel M.A.;
RT   "Gramicidin S biosynthesis operon containing the structural genes grsA and
RT   grsB has an open reading frame encoding a protein homologous to fatty acid
RT   thioesterases.";
RL   J. Bacteriol. 171:5422-5429(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
RC   STRAIN=ATCC 9999 / DSM 2895 / JCM 8504 / NBRC 15520 / NCIMB 7096 / NCTC
RC   7096;
RX   PubMed=7512553; DOI=10.1128/jb.176.8.2458-2462.1994;
RA   Borchert S., Stachelhaus T., Marahiel M.A.;
RT   "Induction of surfactin production in Bacillus subtilis by gsp, a gene
RT   located upstream of the gramicidin S operon in Bacillus brevis.";
RL   J. Bacteriol. 176:2458-2462(1994).
CC   -!- FUNCTION: Probable thioesterase involved in the biosynthesis of
CC       gramicidin S.
CC   -!- PATHWAY: Antibiotic biosynthesis; gramicidin S biosynthesis.
CC   -!- SIMILARITY: Belongs to the thioesterase family. {ECO:0000305}.
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DR   EMBL; M29703; AAA58717.1; -; Genomic_DNA.
DR   EMBL; X15577; CAA33602.1; -; Genomic_DNA.
DR   PIR; B33593; B55218.
DR   RefSeq; WP_043064677.1; NZ_LGUG01000004.1.
DR   AlphaFoldDB; P14686; -.
DR   SMR; P14686; -.
DR   STRING; 47500.AF333_17655; -.
DR   ESTHER; bacbr-grst; Thioesterase.
DR   OrthoDB; 1119700at2; -.
DR   UniPathway; UPA00102; -.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR012223; TEII.
DR   InterPro; IPR001031; Thioesterase.
DR   PANTHER; PTHR11487; PTHR11487; 1.
DR   Pfam; PF00975; Thioesterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Hydrolase.
FT   CHAIN           1..256
FT                   /note="Gramicidin S biosynthesis protein GrsT"
FT                   /id="PRO_0000180362"
FT   ACT_SITE        95
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   256 AA;  29224 MW;  2694EAE1C33E1181 CRC64;
     MTFISQVNKW FVNANVNSAA KLRLFCIPYA GGGASAFYEW SHFFPKEIEV CSIQLPGREN
     RGAEVPLTNL QQIVEIVAEE IQPLINIPFA FLGHSMGALI SFELARTIRQ KSNVNPVHLF
     VSGRHAPQIP CAKQDYHLLP DEQFIQELRS LNGTPEIVLQ DAEMMSILLP RLRADFSVCG
     SYQYKNDEPF ECPITAFGGK NDNGVTYQSL EAWREQTKRE FSVCMYPGDH FFLYESKYEM
     IEFMCKQLRL VLAPKI