GRXC8_ARATH
ID GRXC8_ARATH Reviewed; 140 AA.
AC Q8LF89; B0FL43; Q9FMU3;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 12-DEC-2006, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Glutaredoxin-C8;
DE Short=AtGrxC8;
DE AltName: Full=Protein ROXY 2;
GN Name=GRXC8; Synonyms=ROXY2; OrderedLocusNames=At5g14070; ORFNames=MUA22.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18036205; DOI=10.1111/j.1365-313x.2007.03375.x;
RA Xing S., Zachgo S.;
RT "ROXY1 and ROXY2, two Arabidopsis glutaredoxin genes, are required for
RT anther development.";
RL Plant J. 53:790-801(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15170506; DOI=10.1007/s00018-004-3410-y;
RA Rouhier N., Gelhaye E., Jacquot J.-P.;
RT "Plant glutaredoxins: still mysterious reducing systems.";
RL Cell. Mol. Life Sci. 61:1266-1277(2004).
RN [7]
RP GENE FAMILY.
RX PubMed=16720602; DOI=10.1093/jxb/erl001;
RA Rouhier N., Couturier J., Jacquot J.-P.;
RT "Genome-wide analysis of plant glutaredoxin systems.";
RL J. Exp. Bot. 57:1685-1696(2006).
RN [8]
RP GENE FAMILY.
RX PubMed=19218396; DOI=10.1105/tpc.108.064477;
RA Li S., Lauri A., Ziemann M., Busch A., Bhave M., Zachgo S.;
RT "Nuclear activity of ROXY1, a glutaredoxin interacting with TGA factors, is
RT required for petal development in Arabidopsis thaliana.";
RL Plant Cell 21:429-441(2009).
RN [9]
RP INTERACTION WITH TGA9 AND TGA10, DEVELOPMENTAL STAGE, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=20805327; DOI=10.1104/pp.110.159111;
RA Murmu J., Bush M.J., Delong C., Li S., Xu M., Khan M., Malcolmson C.,
RA Fobert P.R., Zachgo S., Hepworth S.R.;
RT "Arabidopsis basic leucine-zipper transcription factors TGA9 and TGA10
RT interact with floral glutaredoxins ROXY1 and ROXY2 and are redundantly
RT required for anther development.";
RL Plant Physiol. 154:1492-1504(2010).
CC -!- FUNCTION: Has a glutathione-disulfide oxidoreductase activity in the
CC presence of NADPH and glutathione reductase. Reduces low molecular
CC weight disulfides and proteins (By similarity). Involved in flower
CC development. {ECO:0000250, ECO:0000269|PubMed:18036205}.
CC -!- SUBUNIT: Interacts with TGA9 and TGA10 in the nucleus.
CC {ECO:0000269|PubMed:20805327}.
CC -!- INTERACTION:
CC Q8LF89; Q9FYK5: ESR2; NbExp=4; IntAct=EBI-4434651, EBI-1536925;
CC Q8LF89; Q9LYC1: GID1B; NbExp=3; IntAct=EBI-4434651, EBI-963686;
CC Q8LF89; O23160: MYB73; NbExp=3; IntAct=EBI-4434651, EBI-25506855;
CC Q8LF89; Q9LF53: RGL3; NbExp=3; IntAct=EBI-4434651, EBI-15681313;
CC Q8LF89; Q39237: TGA1; NbExp=3; IntAct=EBI-4434651, EBI-541351;
CC Q8LF89; P43273: TGA2; NbExp=2; IntAct=EBI-4434651, EBI-541307;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P17695}. Nucleus
CC {ECO:0000269|PubMed:20805327}.
CC -!- TISSUE SPECIFICITY: Weakly expressed throughout the inflorescence
CC apical meristem and in young buds (PubMed:18036205). Localized to the
CC tapetum and middle layers (PubMed:20805327).
CC {ECO:0000269|PubMed:18036205, ECO:0000269|PubMed:20805327}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Defection in sporogenous
CC cell formation in adaxial anther lobes when associated with the
CC disruption of GRXC7/ROXY1 leading to fertility defects.
CC {ECO:0000269|PubMed:18036205}.
CC -!- SIMILARITY: Belongs to the glutaredoxin family. CC-type subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM61544.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; EU332351; ABY55157.1; -; mRNA.
DR EMBL; AB007650; BAB08286.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91984.1; -; Genomic_DNA.
DR EMBL; AF361802; AAK32815.1; -; mRNA.
DR EMBL; AY055101; AAL05901.1; -; mRNA.
DR EMBL; AY084985; AAM61544.1; ALT_INIT; mRNA.
DR RefSeq; NP_196911.1; NM_121410.3.
DR AlphaFoldDB; Q8LF89; -.
DR SMR; Q8LF89; -.
DR BioGRID; 16534; 16.
DR IntAct; Q8LF89; 17.
DR STRING; 3702.AT5G14070.1; -.
DR PaxDb; Q8LF89; -.
DR PRIDE; Q8LF89; -.
DR EnsemblPlants; AT5G14070.1; AT5G14070.1; AT5G14070.
DR GeneID; 831256; -.
DR Gramene; AT5G14070.1; AT5G14070.1; AT5G14070.
DR KEGG; ath:AT5G14070; -.
DR Araport; AT5G14070; -.
DR TAIR; locus:2174718; AT5G14070.
DR eggNOG; KOG1752; Eukaryota.
DR HOGENOM; CLU_026126_6_1_1; -.
DR InParanoid; Q8LF89; -.
DR OMA; MERALYK; -.
DR OrthoDB; 1535999at2759; -.
DR PhylomeDB; Q8LF89; -.
DR PRO; PR:Q8LF89; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8LF89; baseline and differential.
DR Genevisible; Q8LF89; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0097573; F:glutathione oxidoreductase activity; IEA:InterPro.
DR GO; GO:0048653; P:anther development; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEP:TAIR.
DR InterPro; IPR011905; GlrX-like_pln_2.
DR InterPro; IPR002109; Glutaredoxin.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR10168; PTHR10168; 1.
DR Pfam; PF00462; Glutaredoxin; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR TIGRFAMs; TIGR02189; GlrX-like_plant; 1.
DR PROSITE; PS51354; GLUTAREDOXIN_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Disulfide bond; Electron transport; Nucleus;
KW Redox-active center; Reference proteome; Transport.
FT CHAIN 1..140
FT /note="Glutaredoxin-C8"
FT /id="PRO_0000268715"
FT DOMAIN 31..139
FT /note="Glutaredoxin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00686"
FT MOTIF 137..140
FT /note="Responsive for interaction with TGA factors"
FT /evidence="ECO:0000250"
FT DISULFID 51..54
FT /note="Redox-active"
FT /evidence="ECO:0000250"
FT CONFLICT 68
FT /note="P -> R (in Ref. 5; AAM61544)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 140 AA; 14970 MW; 187A17318217DF57 CRC64;
MQYKTETRGS LSYNNNSKVM NNMNVFPSET LAKIESMAAE NAVVIFSVST CCMCHAIKRL
FRGMGVSPAV HELDLLPYGV EIHRALLRLL GCSSGGATSP GALPVVFIGG KMVGAMERVM
ASHINGSLVP LLKDAGALWL
