GS1_DROME
ID GS1_DROME Reviewed; 231 AA.
AC Q94529; O02536; Q494L7; Q9VR01;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2001, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Probable pseudouridine-5'-phosphatase;
DE EC=3.1.3.96;
DE AltName: Full=GS1-like protein;
DE AltName: Full=Pseudouridine-5'-monophosphatase;
DE Short=5'-PsiMPase;
GN Name=Gs1l; ORFNames=CG15441;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=Canton-S; TISSUE=Embryo;
RX PubMed=9055824; DOI=10.1016/s0378-1119(96)00656-7;
RA Soehnge H., Huang X., Becker M., Conover D., Stern M.;
RT "Cloning and sequencing of ribosomal protein L27a and a gene similar to
RT human GS1 in Drosophila.";
RL Gene 185:257-263(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Dephosphorylates pseudouridine 5'-phosphate, a potential
CC intermediate in rRNA degradation. {ECO:0000250|UniProtKB:Q08623}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + psi-UMP = phosphate + pseudouridine;
CC Xref=Rhea:RHEA:10944, ChEBI:CHEBI:15377, ChEBI:CHEBI:17802,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58380; EC=3.1.3.96;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily.
CC CbbY/CbbZ/Gph/YieH family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC47471.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAC47474.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U66355; AAC47473.1; -; Genomic_DNA.
DR EMBL; U66355; AAC47474.1; ALT_INIT; Genomic_DNA.
DR EMBL; U66356; AAC47470.1; -; mRNA.
DR EMBL; U66356; AAC47471.1; ALT_INIT; mRNA.
DR EMBL; AE014134; AAF51007.1; -; Genomic_DNA.
DR EMBL; BT023759; AAZ41767.1; -; mRNA.
DR PIR; JC6201; JC6201.
DR RefSeq; NP_477228.1; NM_057880.5.
DR AlphaFoldDB; Q94529; -.
DR SMR; Q94529; -.
DR STRING; 7227.FBpp0077143; -.
DR PaxDb; Q94529; -.
DR PRIDE; Q94529; -.
DR DNASU; 33653; -.
DR EnsemblMetazoa; FBtr0077453; FBpp0077143; FBgn0019982.
DR GeneID; 33653; -.
DR KEGG; dme:Dmel_CG15441; -.
DR CTD; 33653; -.
DR FlyBase; FBgn0019982; Gs1l.
DR VEuPathDB; VectorBase:FBgn0019982; -.
DR eggNOG; KOG2914; Eukaryota.
DR GeneTree; ENSGT00390000014753; -.
DR HOGENOM; CLU_045011_13_0_1; -.
DR InParanoid; Q94529; -.
DR OMA; DSERVYT; -.
DR PhylomeDB; Q94529; -.
DR Reactome; R-DME-73614; Pyrimidine salvage.
DR BioGRID-ORCS; 33653; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33653; -.
DR PRO; PR:Q94529; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0019982; Expressed in adult Malpighian tubule (Drosophila) and 25 other tissues.
DR ExpressionAtlas; Q94529; baseline and differential.
DR Genevisible; Q94529; DM.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016791; F:phosphatase activity; IBA:GO_Central.
DR GO; GO:1990738; F:pseudouridine 5'-phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0009117; P:nucleotide metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0042060; P:wound healing; HMP:FlyBase.
DR CDD; cd07529; HAD_AtGPP-like; 1.
DR Gene3D; 1.10.150.240; -; 1.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR045228; Gpp1/Gpp2-like.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR006439; HAD-SF_hydro_IA.
DR InterPro; IPR041492; HAD_2.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR023198; PGP-like_dom2.
DR Pfam; PF13419; HAD_2; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR01509; HAD-SF-IA-v3; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Magnesium; Metal-binding; Nucleotide metabolism;
KW Reference proteome.
FT CHAIN 1..231
FT /note="Probable pseudouridine-5'-phosphatase"
FT /id="PRO_0000108069"
FT ACT_SITE 15
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 17
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 15
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 17
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT VARIANT 176..177
FT /note="FE -> VQ (in strain: Canton-S)"
SQ SEQUENCE 231 AA; 25785 MW; 3D4421C9665B69D4 CRC64;
MANKVLRKVT HCVFDMDGLL LDTERLYTVA TEMILEPYGK TYPFEIKEQV MGLQTEPLAR
FMVEHYELPM SWEEYARQQR ANTEILMRNA QLMPGAERLL RHLHANKVPF CLATSSGADM
VELKTAQHRE LFSLFNHKVC GSSDKEVVNG KPAPDIFLVA AGRFGVPPKP SDCLVFEDSP
NGVTAANSAG MQVVMVPDPR LSQEKTSHAT QVLASLADFK PEQFGLPAFT D
