3SA1_NAJOX
ID 3SA1_NAJOX Reviewed; 60 AA.
AC P01451;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Cytotoxin 1;
DE AltName: Full=Cytotoxin I;
DE Short=CTI;
OS Naja oxiana (Central Asian cobra) (Oxus cobra).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX NCBI_TaxID=8657;
RN [1]
RP PROTEIN SEQUENCE, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RA Grishin E.V., Sukhikh A.P., Adamovich T.B., Ovchinnikov Y.A.;
RT "Isolation, properties and sequence determination of the two cytotoxins
RT from the venom of the Middle-Asian cobra Naja Naja oxiana.";
RL Bioorg. Khim. 2:1018-1034(1976).
RN [2]
RP STRUCTURE BY NMR, AND DISULFIDE BONDS.
RX PubMed=15584897; DOI=10.1042/bj20041814;
RA Dubovskii P.V., Lesovoy D.M., Dubinnyi M.A., Konshina A.G., Utkin Y.N.,
RA Efremov R.G., Arseniev A.S.;
RT "Interaction of three-finger toxins with phospholipid membranes: comparison
RT of S- and P-type cytotoxins.";
RL Biochem. J. 387:807-815(2005).
CC -!- FUNCTION: This three-finger cytotoxin is a basic protein that interacts
CC and penetrates into the cell membrane, with the tips of all the three
CC loops. Cytotoxins which have a 'Pro-30' (P-type) interacts with
CC membrane stronger that those which have a Ser-28 (S-type). CTI
CC interacts with membrane weaker than CTII.
CC -!- SUBUNIT: Monomer in solution; Homodimer and oligomer in the presence of
CC negatively charged lipids forming a pore with a size ranging between 20
CC and 30 Angstroms. {ECO:0000250|UniProtKB:P60301}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}. Target cell
CC membrane {ECO:0000250|UniProtKB:P60301}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC -!- MISCELLANEOUS: Is classified as a S-type cytotoxin, since a serine
CC residue stands at position 27 (Ser-29 in standard classification).
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC subfamily. Type IA cytotoxin sub-subfamily. {ECO:0000305}.
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DR PIR; A01716; H3NJ1R.
DR PDB; 1RL5; NMR; -; A=1-60.
DR PDB; 1ZAD; NMR; -; A=1-60.
DR PDB; 5NPN; NMR; -; A=1-60.
DR PDB; 5NQ4; NMR; -; A=1-60.
DR PDB; 5T8A; NMR; -; A=1-60.
DR PDBsum; 1RL5; -.
DR PDBsum; 1ZAD; -.
DR PDBsum; 5NPN; -.
DR PDBsum; 5NQ4; -.
DR PDBsum; 5T8A; -.
DR AlphaFoldDB; P01451; -.
DR BMRB; P01451; -.
DR SMR; P01451; -.
DR EvolutionaryTrace; P01451; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR CDD; cd00206; snake_toxin; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR003572; Cytotoxin_Cobra.
DR InterPro; IPR003571; Snake_3FTx.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR018354; Snake_toxin_con_site.
DR PRINTS; PR00282; CYTOTOXIN.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00272; SNAKE_TOXIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cardiotoxin; Cytolysis; Direct protein sequencing;
KW Disulfide bond; Membrane; Secreted; Target cell membrane; Target membrane;
KW Toxin.
FT CHAIN 1..60
FT /note="Cytotoxin 1"
FT /evidence="ECO:0000269|Ref.1"
FT /id="PRO_0000093516"
FT DISULFID 3..21
FT /evidence="ECO:0000269|PubMed:15584897,
FT ECO:0000312|PDB:1RL5, ECO:0000312|PDB:1ZAD,
FT ECO:0000312|PDB:5NPN, ECO:0000312|PDB:5NQ4,
FT ECO:0000312|PDB:5T8A"
FT DISULFID 14..38
FT /evidence="ECO:0000269|PubMed:15584897,
FT ECO:0000312|PDB:1RL5, ECO:0000312|PDB:1ZAD,
FT ECO:0000312|PDB:5NPN, ECO:0000312|PDB:5NQ4,
FT ECO:0000312|PDB:5T8A"
FT DISULFID 42..53
FT /evidence="ECO:0000269|PubMed:15584897,
FT ECO:0000312|PDB:1RL5, ECO:0000312|PDB:1ZAD,
FT ECO:0000312|PDB:5NPN, ECO:0000312|PDB:5NQ4,
FT ECO:0000312|PDB:5T8A"
FT DISULFID 54..59
FT /evidence="ECO:0000269|PubMed:15584897,
FT ECO:0000312|PDB:1RL5, ECO:0000312|PDB:1ZAD,
FT ECO:0000312|PDB:5NPN, ECO:0000312|PDB:5NQ4,
FT ECO:0000312|PDB:5T8A"
FT STRAND 2..4
FT /evidence="ECO:0007829|PDB:1RL5"
FT STRAND 11..13
FT /evidence="ECO:0007829|PDB:1RL5"
FT STRAND 20..29
FT /evidence="ECO:0007829|PDB:1RL5"
FT STRAND 34..41
FT /evidence="ECO:0007829|PDB:1RL5"
FT STRAND 47..54
FT /evidence="ECO:0007829|PDB:1RL5"
SQ SEQUENCE 60 AA; 6821 MW; C0E8AE8AC6A86F11 CRC64;
LKCNKLVPIA YKTCPEGKNL CYKMFMMSDL TIPVKRGCID VCPKNSLLVK YVCCNTDRCN
