GSA_ORYSJ
ID GSA_ORYSJ Reviewed; 478 AA.
AC Q6YZE2; Q0J464;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic;
DE Short=GSA;
DE EC=5.4.3.8;
DE AltName: Full=Glutamate-1-semialdehyde aminotransferase;
DE Short=GSA-AT;
DE Flags: Precursor;
GN Name=GSA; OrderedLocusNames=Os08g0532200, LOC_Os08g41990;
GN ORFNames=OsJ_28060 {ECO:0000312|EMBL:EAZ43454.1}, P0702E04.16;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-4-amino-5-oxopentanoate = 5-aminolevulinate;
CC Xref=Rhea:RHEA:14265, ChEBI:CHEBI:57501, ChEBI:CHEBI:356416;
CC EC=5.4.3.8;
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000250};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC biosynthesis.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the class-III pyridoxal-phosphate-dependent
CC aminotransferase family. HemL subfamily. {ECO:0000305}.
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DR EMBL; AP005529; BAD11647.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF24251.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT06404.1; -; Genomic_DNA.
DR EMBL; CM000145; EAZ43454.1; -; Genomic_DNA.
DR EMBL; AK064826; BAG89226.1; -; mRNA.
DR EMBL; AK104694; BAG96887.1; -; mRNA.
DR RefSeq; XP_015650872.1; XM_015795386.1.
DR AlphaFoldDB; Q6YZE2; -.
DR SMR; Q6YZE2; -.
DR STRING; 4530.OS08T0532200-01; -.
DR PaxDb; Q6YZE2; -.
DR PRIDE; Q6YZE2; -.
DR EnsemblPlants; Os08t0532200-01; Os08t0532200-01; Os08g0532200.
DR EnsemblPlants; Os08t0532200-03; Os08t0532200-03; Os08g0532200.
DR GeneID; 4346136; -.
DR Gramene; Os08t0532200-01; Os08t0532200-01; Os08g0532200.
DR Gramene; Os08t0532200-03; Os08t0532200-03; Os08g0532200.
DR KEGG; osa:4346136; -.
DR eggNOG; KOG1401; Eukaryota.
DR HOGENOM; CLU_016922_1_5_1; -.
DR InParanoid; Q6YZE2; -.
DR OMA; WGPLIFG; -.
DR OrthoDB; 1160614at2759; -.
DR PlantReactome; R-OSA-4827054; Tetrapyrrole biosynthesis I.
DR UniPathway; UPA00251; UER00317.
DR UniPathway; UPA00668; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR ExpressionAtlas; Q6YZE2; baseline and differential.
DR Genevisible; Q6YZE2; OS.
DR GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR GO; GO:0042286; F:glutamate-1-semialdehyde 2,1-aminomutase activity; IEA:UniProtKB-EC.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR GO; GO:0008483; F:transaminase activity; IEA:InterPro.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00610; OAT_like; 1.
DR Gene3D; 3.40.640.10; -; 1.
DR Gene3D; 3.90.1150.10; -; 1.
DR HAMAP; MF_00375; HemL_aminotrans_3; 1.
DR InterPro; IPR004639; 4pyrrol_synth_GluAld_NH2Trfase.
DR InterPro; IPR005814; Aminotrans_3.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR Pfam; PF00202; Aminotran_3; 1.
DR SUPFAM; SSF53383; SSF53383; 1.
DR TIGRFAMs; TIGR00713; hemL; 1.
DR PROSITE; PS00600; AA_TRANSFER_CLASS_3; 1.
PE 2: Evidence at transcript level;
KW Chlorophyll biosynthesis; Chloroplast; Isomerase; Plastid;
KW Porphyrin biosynthesis; Pyridoxal phosphate; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..40
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 41..478
FT /note="Glutamate-1-semialdehyde 2,1-aminomutase,
FT chloroplastic"
FT /id="PRO_0000247473"
FT REGION 15..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 318
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 478 AA; 50238 MW; 672E23E08B8A0F11 CRC64;
MAGAAAASAA AAAVASGISA RPVAPRPSPS RARAPRSVVR AAISVEKGEK AYTVEKSEEI
FNAAKELMPG GVNSPVRAFK SVGGQPIVFD SVKGSRMWDV DGNEYIDYVG SWGPAIIGHA
DDTVNAALIE TLKKGTSFGA PCVLENVLAE MVISAVPSIE MVRFVNSGTE ACMGALRLVR
AFTGREKILK FEGCYHGHAD SFLVKAGSGV ATLGLPDSPG VPKGATSETL TAPYNDVEAV
KKLFEENKGQ IAAVFLEPVV GNAGFIPPQP GFLNALRDLT KQDGALLVFD EVMTGFRLAY
GGAQEYFGIT PDVSTLGKII GGGLPVGAYG GRKDIMEMVA PAGPMYQAGT LSGNPLAMTA
GIHTLKRLME PGTYDYLDKI TGDLVRGVLD AGAKTGHEMC GGHIRGMFGF FFTAGPVHNF
GDAKKSDTAK FGRFYRGMLE EGVYLAPSQF EAGFTSLAHT SQDIEKTVEA AAKVLRRI
