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GSA_PSEAE
ID   GSA_PSEAE               Reviewed;         427 AA.
AC   P48247;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   08-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 147.
DE   RecName: Full=Glutamate-1-semialdehyde 2,1-aminomutase;
DE            Short=GSA;
DE            EC=5.4.3.8;
DE   AltName: Full=Glutamate-1-semialdehyde aminotransferase;
DE            Short=GSA-AT;
GN   Name=hemL; OrderedLocusNames=PA3977;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=7565600; DOI=10.1007/bf02191605;
RA   Hungerer C., Troup B., Romling U., Jahn D.;
RT   "Cloning, mapping and characterization of the Pseudomonas aeruginosa hemL
RT   gene.";
RL   Mol. Gen. Genet. 248:375-380(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-4-amino-5-oxopentanoate = 5-aminolevulinate;
CC         Xref=Rhea:RHEA:14265, ChEBI:CHEBI:57501, ChEBI:CHEBI:356416;
CC         EC=5.4.3.8;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC       biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the class-III pyridoxal-phosphate-dependent
CC       aminotransferase family. HemL subfamily. {ECO:0000305}.
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DR   EMBL; X82072; CAA57575.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG07364.1; -; Genomic_DNA.
DR   PIR; G83149; G83149.
DR   PIR; S57898; S57898.
DR   RefSeq; NP_252666.1; NC_002516.2.
DR   RefSeq; WP_003093150.1; NZ_QZGE01000001.1.
DR   PDB; 5I92; X-ray; 1.75 A; A/B/C/D/E/F=1-427.
DR   PDBsum; 5I92; -.
DR   AlphaFoldDB; P48247; -.
DR   SMR; P48247; -.
DR   STRING; 287.DR97_3888; -.
DR   PaxDb; P48247; -.
DR   PRIDE; P48247; -.
DR   EnsemblBacteria; AAG07364; AAG07364; PA3977.
DR   GeneID; 880859; -.
DR   KEGG; pae:PA3977; -.
DR   PATRIC; fig|208964.12.peg.4169; -.
DR   PseudoCAP; PA3977; -.
DR   HOGENOM; CLU_016922_1_5_6; -.
DR   InParanoid; P48247; -.
DR   OMA; WGPLIFG; -.
DR   PhylomeDB; P48247; -.
DR   BioCyc; PAER208964:G1FZ6-4051-MON; -.
DR   UniPathway; UPA00251; UER00317.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042286; F:glutamate-1-semialdehyde 2,1-aminomutase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   GO; GO:0008483; F:transaminase activity; IEA:InterPro.
DR   GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00610; OAT_like; 1.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   HAMAP; MF_00375; HemL_aminotrans_3; 1.
DR   InterPro; IPR004639; 4pyrrol_synth_GluAld_NH2Trfase.
DR   InterPro; IPR005814; Aminotrans_3.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   Pfam; PF00202; Aminotran_3; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   TIGRFAMs; TIGR00713; hemL; 1.
DR   PROSITE; PS00600; AA_TRANSFER_CLASS_3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Isomerase; Porphyrin biosynthesis;
KW   Pyridoxal phosphate; Reference proteome.
FT   CHAIN           1..427
FT                   /note="Glutamate-1-semialdehyde 2,1-aminomutase"
FT                   /id="PRO_0000120433"
FT   MOD_RES         265
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        157
FT                   /note="A -> S (in Ref. 1; CAA57575)"
FT                   /evidence="ECO:0000305"
FT   HELIX           3..11
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   TURN            12..14
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           16..18
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           22..25
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   TURN            27..29
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          36..41
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          43..46
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          51..56
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           57..59
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           69..79
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           90..102
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          107..113
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           115..130
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          134..139
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           147..149
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          150..153
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          161..165
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           170..173
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          176..180
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           184..194
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           195..197
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          198..203
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          205..207
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          209..211
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           219..230
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          233..237
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   TURN            239..244
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           249..254
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          259..264
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           265..268
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          274..278
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           280..283
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   TURN            287..289
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          290..292
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          295..297
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           302..314
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           320..341
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          347..351
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   STRAND          354..358
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           368..373
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           376..388
FT                   /evidence="ECO:0007829|PDB:5I92"
FT   HELIX           409..424
FT                   /evidence="ECO:0007829|PDB:5I92"
SQ   SEQUENCE   427 AA;  45398 MW;  670EB87D144E2D28 CRC64;
     MSRSETLFNN AQKHIPGGVN SPVRAFKSVG GTPLFFKHAE GAYVLDEDDK RYVDYVGSWG
     PMILGHSHPD VLDAVRRQLD HGLSYGAPTA LEVEMADLVC SMVPSMEMVR MVSSGTEATM
     SAIRLARGYT GRDSIIKFEG CYHGHSDSLL VKAGSGALTF GVPNSPGVPA AFAKHTLTLP
     FNDIEAVRKT LGEVGKEVAC IIVEPVAGNM NCVPPAPGFL EGLREACDEH GVVLIFDEVM
     TGFRVALGGA QAYYGVTPDL STFGKIIGGG MPVGAFGGKR EIMQQISPLG PVYQAGTLSG
     NPLAMAAGLT TLRLISRPGF HDELTAYTTR MLDGLQQRAD AAGIPFVTTQ AGGMFGLYFS
     GADAIVTFED VMASDVERFK RFFHLMLDGG VYLAPSAFEA GFTSIAHGDK ELEITLNAAE
     KAFAALK
 
 
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