GSA_SOYBN
ID GSA_SOYBN Reviewed; 466 AA.
AC P45621;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 25-MAY-2022, entry version 126.
DE RecName: Full=Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic;
DE Short=GSA;
DE EC=5.4.3.8;
DE AltName: Full=Glutamate-1-semialdehyde aminotransferase;
DE Short=GSA-AT;
DE Flags: Precursor;
GN Name=GSA1; Synonyms=GSA;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Root nodule;
RX PubMed=8278535; DOI=10.1104/pp.102.3.829;
RA Sangwan I., O'Brian M.R.;
RT "Expression of the soybean (Glycine max) glutamate 1-semialdehyde
RT aminotransferase gene in symbiotic root nodules.";
RL Plant Physiol. 102:829-834(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Essex;
RX PubMed=7706283; DOI=10.1074/jbc.270.13.7387;
RA Frustaci J.M., Sangwan I., O'Brian M.R.;
RT "gsa1 is a universal tetrapyrrole synthesis gene in soybean and is
RT regulated by a GAGA element.";
RL J. Biol. Chem. 270:7387-7393(1995).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-4-amino-5-oxopentanoate = 5-aminolevulinate;
CC Xref=Rhea:RHEA:14265, ChEBI:CHEBI:57501, ChEBI:CHEBI:356416;
CC EC=5.4.3.8;
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC biosynthesis.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- TISSUE SPECIFICITY: Strongly expressed in leaves of etiolated plantlets
CC independently of light treatment and, to a much lesser extent, in
CC leaves of mature plants.
CC -!- SIMILARITY: Belongs to the class-III pyridoxal-phosphate-dependent
CC aminotransferase family. HemL subfamily. {ECO:0000305}.
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DR EMBL; L12453; AAA33968.1; -; mRNA.
DR EMBL; U20260; AAC48996.1; -; Genomic_DNA.
DR PIR; JQ2263; JQ2263.
DR RefSeq; NP_001238043.1; NM_001251114.1.
DR AlphaFoldDB; P45621; -.
DR SMR; P45621; -.
DR STRING; 3847.GLYMA06G00510.1; -.
DR PRIDE; P45621; -.
DR ProMEX; P45621; -.
DR EnsemblPlants; KRH51376; KRH51376; GLYMA_06G002900.
DR GeneID; 547795; -.
DR Gramene; KRH51376; KRH51376; GLYMA_06G002900.
DR KEGG; gmx:547795; -.
DR eggNOG; KOG1401; Eukaryota.
DR HOGENOM; CLU_016922_1_5_1; -.
DR InParanoid; P45621; -.
DR OMA; AQTWRFP; -.
DR OrthoDB; 1160614at2759; -.
DR UniPathway; UPA00251; UER00317.
DR UniPathway; UPA00668; -.
DR Proteomes; UP000008827; Chromosome 6.
DR Genevisible; P45621; GM.
DR GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR GO; GO:0042286; F:glutamate-1-semialdehyde 2,1-aminomutase activity; IEA:UniProtKB-EC.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR GO; GO:0008483; F:transaminase activity; IEA:InterPro.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00610; OAT_like; 1.
DR Gene3D; 3.40.640.10; -; 1.
DR Gene3D; 3.90.1150.10; -; 1.
DR HAMAP; MF_00375; HemL_aminotrans_3; 1.
DR InterPro; IPR004639; 4pyrrol_synth_GluAld_NH2Trfase.
DR InterPro; IPR005814; Aminotrans_3.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR Pfam; PF00202; Aminotran_3; 1.
DR SUPFAM; SSF53383; SSF53383; 1.
DR TIGRFAMs; TIGR00713; hemL; 1.
DR PROSITE; PS00600; AA_TRANSFER_CLASS_3; 1.
PE 2: Evidence at transcript level;
KW Chlorophyll biosynthesis; Chloroplast; Isomerase; Plastid;
KW Porphyrin biosynthesis; Pyridoxal phosphate; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..28
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 29..466
FT /note="Glutamate-1-semialdehyde 2,1-aminomutase,
FT chloroplastic"
FT /id="PRO_0000001260"
FT MOD_RES 306
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 466 AA; 49646 MW; 5523012AE808DE72 CRC64;
MAVSAITGAR LTLGMSLSSS TRSRTVAMAV SIDPKTDNKL TLTKSEEAFA AAKELMPGGV
NSPVRAFKSV GGQPIVIDSV KGSRMWDIDG NEYIDYVGSW GPAIIGHADD QVLAALGETM
KKGTSFGAPC LLENTLAELV IDAVPSIEMV RFVNSGTEAC MGALRLARAY TGREKIIKFE
GCYHGHADPF LVKAGSGVAT LGLPDSPGVP KAATFETLTA PYNDTEAIEK LFEANKGEIA
AVFLEPVVGN AGFIVPKPDF HSFLRKITKE NNTLLVFDEV MTGFRLSYGG AQEYFGITPD
ITTLGKIIGG GLPVGAYGGR RDIMEKVAPA GPMYQAGTLS GNPLAMTAGI ETLQRIKEPG
TYEYLDKITG ELVEGIIEAG KRAGHAICGG HIRGMFGFFF TEGPVYNFAD AKKSDTAKFA
RFFWGMLAEG VYLAPSQFEA GFTSLAHTSD DIKKTIAAAE KVFREI
