GSF2_SCHPO
ID GSF2_SCHPO Reviewed; 1563 AA.
AC Q9P6S0; O59777; Q96WU8;
DT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 3.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Galactose-specific cell agglutination protein gsf2 {ECO:0000305};
DE AltName: Full=Galactose-specific flocculin {ECO:0000303|PubMed:22098069};
DE AltName: Full=Pombe flocculin 1 {ECO:0000303|PubMed:23236291};
DE Flags: Precursor;
GN Name=gsf2 {ECO:0000303|PubMed:22098069};
GN Synonyms=pfl1 {ECO:0000303|PubMed:23236291};
GN ORFNames=SPCC1742.01 {ECO:0000312|PomBase:SPCC1742.01}, SPCC1795.13,
GN SPCPB16A4.07c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, AND REPEATS.
RX PubMed=22098069; DOI=10.1111/j.1365-2958.2011.07908.x;
RA Matsuzawa T., Morita T., Tanaka N., Tohda H., Takegawa K.;
RT "Identification of a galactose-specific flocculin essential for non-sexual
RT flocculation and filamentous growth in Schizosaccharomyces pombe.";
RL Mol. Microbiol. 82:1531-1544(2011).
RN [3]
RP FUNCTION.
RX PubMed=23236291; DOI=10.1371/journal.pgen.1003104;
RA Kwon E.J., Laderoute A., Chatfield-Reed K., Vachon L., Karagiannis J.,
RA Chua G.;
RT "Deciphering the transcriptional-regulatory network of flocculation in
RT Schizosaccharomyces pombe.";
RL PLoS Genet. 8:E1003104-E1003104(2012).
CC -!- FUNCTION: Galactose-specific adhesion protein essential for non-sexual
CC flocculation and filamentous growth. Required for adhesion and
CC filamentous growth through recognition of galactose residues on cell
CC surface glycoconjugates (PubMed:22098069). Induces flocculation when
CC overexpressed (PubMed:23236291). {ECO:0000269|PubMed:22098069,
CC ECO:0000269|PubMed:23236291}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Lipid-anchor, GPI-
CC anchor {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Abolishes adhesion and invasive growth.
CC {ECO:0000269|PubMed:22098069}.
CC -!- SIMILARITY: Belongs to the mam3/map4 family. {ECO:0000305}.
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DR EMBL; CU329672; CAC39326.1; -; Genomic_DNA.
DR PIR; T41130; T41130.
DR RefSeq; NP_588031.3; NM_001023022.3.
DR AlphaFoldDB; Q9P6S0; -.
DR BioGRID; 275855; 13.
DR STRING; 4896.SPCC1742.01.1; -.
DR PaxDb; Q9P6S0; -.
DR EnsemblFungi; SPCC1742.01.1; SPCC1742.01.1:pep; SPCC1742.01.
DR GeneID; 2539287; -.
DR KEGG; spo:SPCC1742.01; -.
DR PomBase; SPCC1742.01; gsf2.
DR VEuPathDB; FungiDB:SPCC1742.01; -.
DR eggNOG; ENOG502QQXP; Eukaryota.
DR HOGENOM; CLU_245825_0_0_1; -.
DR OMA; DVIHPAV; -.
DR PRO; PR:Q9P6S0; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0031362; C:anchored component of external side of plasma membrane; NAS:PomBase.
DR GO; GO:0098631; F:cell adhesion mediator activity; TAS:PomBase.
DR GO; GO:0036349; P:galactose-specific flocculation; IMP:PomBase.
PE 3: Inferred from homology;
KW Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Repeat; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..1539
FT /note="Galactose-specific cell agglutination protein gsf2"
FT /id="PRO_0000014207"
FT PROPEP 1540..1563
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000014208"
FT REPEAT 203..280
FT /note="1-1"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 281..358
FT /note="1-2"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 359..436
FT /note="1-3"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 437..514
FT /note="1-4"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 515..592
FT /note="1-5"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 593..670
FT /note="1-6"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 671..750
FT /note="1-7"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 751..825
FT /note="1-8"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 826..869
FT /note="2-1"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 870..913
FT /note="2-2"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 914..957
FT /note="2-3"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 958..1001
FT /note="2-4"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 1002..1045
FT /note="2-5"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 1046..1089
FT /note="2-6"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 1090..1133
FT /note="2-7"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 1134..1140
FT /note="2-8"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REPEAT 1141..1162
FT /note="3-1"
FT /evidence="ECO:0000305"
FT REPEAT 1163..1184
FT /note="3-2"
FT /evidence="ECO:0000305"
FT REPEAT 1185..1200
FT /note="3-3"
FT /evidence="ECO:0000305"
FT REPEAT 1201..1221
FT /note="3-4"
FT /evidence="ECO:0000305"
FT REPEAT 1223..1244
FT /note="3-5"
FT /evidence="ECO:0000305"
FT REPEAT 1245..1266
FT /note="3-6"
FT /evidence="ECO:0000305"
FT REPEAT 1267..1282
FT /note="3-7"
FT /evidence="ECO:0000305"
FT REPEAT 1283..1304
FT /note="3-8"
FT /evidence="ECO:0000305"
FT REPEAT 1305..1326
FT /note="3-9"
FT /evidence="ECO:0000305"
FT REPEAT 1327..1348
FT /note="3-10"
FT /evidence="ECO:0000305"
FT REPEAT 1349..1364
FT /note="3-11"
FT /evidence="ECO:0000305"
FT REPEAT 1365..1386
FT /note="3-12"
FT /evidence="ECO:0000305"
FT REPEAT 1387..1397
FT /note="3-13"
FT /evidence="ECO:0000305"
FT REGION 203..825
FT /note="8 X 78 AA approximate tandem repeats"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REGION 826..1140
FT /note="8 X 44 AA approximate tandem repeats"
FT /evidence="ECO:0000305|PubMed:22098069"
FT REGION 1135..1393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1141..1397
FT /note="13 X 22 AA approximate tandem repeats"
FT /evidence="ECO:0000305"
FT LIPID 1539
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 224
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 263
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 302
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 341
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 380
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 419
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 458
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 497
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 536
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 575
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 614
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 653
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 784
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1510
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1516
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1529
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1532
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 1563 AA; 157643 MW; 3F6A2080630CD1A9 CRC64;
MSVRRFLSTS ARALLFTAAL LPSLTSGLPS GNVRILQKGM EPEDYLSSAS QNEVPHDISL
PKTELADPNF LVDDMPTLLG RDAAVDPSMF TSTFTVKNGN DANYITASPV SNDASMTAIS
TFTSGKEASY AIQASPSTFL PDSTTTSGSQ VSNAVEASST FVADTTSTSC NPATVLIVTT
SGSTSTSCPP PTTILIVTVP TTTTTTTVGY PGSVTTTLTG TPSNGTVIDT VEVPTTTNYG
YTTITTGYTG STTLTTTVPH SGNETGPTTV YVETPYPTTV TTTTTVGYPG SVTTTLTGAP
SNGTVIDTVE VPTTTNYGYT TVTTGYTGST TLTTTVPHSG NETGPTTVYV ETPYPTTVTT
TTTVGYPGSV TTTLTGAPSN GTVIDTVEVP TTTNYGYTTV TTGYTGSTTL TTTVPHSGNE
TGPTTVYVET PYPTTVTTTT TVGYPGSVTT TLTGAPSNGT VIDTVEIPTT TNYGYTTITT
GYTGSTTLTT TVPHSGNETG PTTVYVETPY PTTVTTTTTV GYPGSVTTTL TGAPSNGTVI
DTVEVPTTTN YGYTTITTGY TGSTTLTTTV PHSGNETGPT TVYVETPYPT TVTTTTTVGY
PGSVTTTLTG APSNGTVIDT VEVPTTTNYG YTTVTTGYTG STTLTTTVPH SGNETGPTTV
YVETPYPTTV TTTTTVGYSG SVTTTLTGSG SNSIVTETVD VPTTTSVNYG YTTITTGWTG
STTLTSIVTH SGSETGPTTV YIETPSVSAT TTTTTIGYSG SLTTTLTGSS GPVVTNTVEI
PYGNSSYIIP TTIVTGTVTT VTTGYTGTET STVTVIPTGT TGTTTVVIQT PTTVTATETD
IVTVTTGYTG TETSTVTVTP TGTSTGTTTV VIQTPTTVTA TETDIVTVTT GYTGTETSTV
TVTPTGTSTG TTTVVIQTPT TVTATETDIV TVTTGYTGTE TSTVTVTPTG TSTGTTTVVI
QTPTTVTATE TDIVTVTTGY TGTETSTVTV TPTGTSTGTT TVVIQTPTTV TATETDIVTV
TTGYTGTETS TVTVTPTGTS TGTTTVVIQT PTTVTATETD IVTVTTGYTG TETSTVTVTP
TGTSTGTTTV VIQTPTTVTA TETDIVTVTT GYTGTETSTV TVTPTGTATG TTTVVINTPT
TTGSEVLPTT GATGTAGTET QLTTATEVQP TTGATGTAGT ETQVTTGTET QATTATETQA
TTATEVQTTT GATGTAGTET QATTATEVQP TTGATGTAGT ETQVTTATEV QPTTGATGTA
GTETQVTTGT ETQATTATET QATTATEVQT TTGATGTAGT ETQATTATEV QPTTGATGTA
GTETQVTTAT EVQPTTGATG TAGTETQVTT GTETQATTAT ETQATTATEV QTTTGATGTA
GTETQVTTAT EVQPTTAVTE TSSSGYYTTI VSSTVVSTVV PGSTVYPVTH VTTTTGVSGE
SSAFTYTTSS TQYEPSTVVT TSYYTTSVYT SAPATETVSS TEAPESSTVT SNPIYQGSGT
STWSTVRQWN GSATYNYTYY TTGGFTGGNN TNVTGLYPSS AGANKPIAYL TFVSLFVYIV
TLI
