GSF2_YEAST
ID GSF2_YEAST Reviewed; 403 AA.
AC Q04697; D6VZC7;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Glucose-signaling factor 2;
DE AltName: Full=Extracellular mutant protein 6;
GN Name=GSF2; Synonyms=ECM6, EFF2; OrderedLocusNames=YML048W;
GN ORFNames=YM9827.04;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP IDENTIFICATION.
RX PubMed=9335584; DOI=10.1093/genetics/147.2.435;
RA Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J.,
RA Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C.,
RA Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M.,
RA Davies J., Klis F.M., Robbins P.W., Bussey H.;
RT "Large scale identification of genes involved in cell surface biosynthesis
RT and architecture in Saccharomyces cerevisiae.";
RL Genetics 147:435-450(1997).
RN [5]
RP FUNCTION.
RX PubMed=9335593; DOI=10.1093/genetics/147.2.557;
RA Sherwood P.W., Carlson M.;
RT "Mutations in GSF1 and GSF2 alter glucose signaling in Saccharomyces
RT cerevisiae.";
RL Genetics 147:557-566(1997).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=10377429; DOI=10.1073/pnas.96.13.7415;
RA Sherwood P.W., Carlson M.;
RT "Efficient export of the glucose transporter Hxt1p from the endoplasmic
RT reticulum requires Gsf2p.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:7415-7420(1999).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: May be involved in the secretion of hexose transporters from
CC the endoplasmic reticulum. Involved in secretion of GAL2 and HXT1.
CC {ECO:0000269|PubMed:10377429, ECO:0000269|PubMed:9335593}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Single-pass type II membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 21400 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z47816; CAA87826.1; -; Genomic_DNA.
DR EMBL; AY692925; AAT92944.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09851.1; -; Genomic_DNA.
DR PIR; S50944; S50944.
DR RefSeq; NP_013664.1; NM_001182405.1.
DR AlphaFoldDB; Q04697; -.
DR BioGRID; 35120; 334.
DR DIP; DIP-4541N; -.
DR IntAct; Q04697; 25.
DR MINT; Q04697; -.
DR STRING; 4932.YML048W; -.
DR iPTMnet; Q04697; -.
DR MaxQB; Q04697; -.
DR PaxDb; Q04697; -.
DR PRIDE; Q04697; -.
DR EnsemblFungi; YML048W_mRNA; YML048W; YML048W.
DR GeneID; 854957; -.
DR KEGG; sce:YML048W; -.
DR SGD; S000004511; GSF2.
DR VEuPathDB; FungiDB:YML048W; -.
DR eggNOG; ENOG502QT03; Eukaryota.
DR HOGENOM; CLU_719597_0_0_1; -.
DR InParanoid; Q04697; -.
DR OMA; IMLAWLY; -.
DR BioCyc; YEAST:G3O-32645-MON; -.
DR PRO; PR:Q04697; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04697; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IDA:SGD.
DR GO; GO:0005741; C:mitochondrial outer membrane; HDA:SGD.
DR GO; GO:0051082; F:unfolded protein binding; IMP:SGD.
DR GO; GO:0006457; P:protein folding; IMP:SGD.
DR GO; GO:0034394; P:protein localization to cell surface; IMP:SGD.
DR InterPro; IPR022757; Gsf2.
DR Pfam; PF11055; Gsf2; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endoplasmic reticulum; Glycoprotein; Membrane;
KW Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..403
FT /note="Glucose-signaling factor 2"
FT /id="PRO_0000083865"
FT TOPO_DOM 1..177
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..403
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT COILED 330..388
FT /evidence="ECO:0000255"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 173
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 403 AA; 45870 MW; 096E07AFFA711ECB CRC64;
MEIYIRLNAD VEHDYAFQVS NEDTINNKIK KIFPSKTGLA DLMVLRPSIF HEKEPVKFYK
SIHPGYLSEG GCLMFHYEAD NEENLEELND SKPLIDQLWP GQLVVPEWKL SKKNIWVYTI
IMLAWLYTDL PDAISPTPGI CLTNQLSRLL IPVAKHMDLP EIAAKLEQEV QANYSSLVAQ
WLFFVMHIFK VGIITLFLKL GIANPISFNP YKLWSLRDLT SPSANGAKNS GGNNNTTDLK
TRLRSLGWIG AKRATYDDYQ TNYYNYVIDK MGGAVAAYRA GAIRKAAAPG IQLVAGEGFQ
SPLEDRFTAS TFTAIKTERK FILSEEYFVE LENNLKKILE EYDGDIGKMN AEIRRFRRFG
IYEPDEKLAS LVKLRREIAD EKEKASNNDA TFGIKKNDLK KSN
