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GSFE_PENAE
ID   GSFE_PENAE              Reviewed;         377 AA.
AC   D7PI19;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   10-AUG-2010, sequence version 1.
DT   03-AUG-2022, entry version 26.
DE   RecName: Full=Short chain dehydrogenase gsfE {ECO:0000303|PubMed:20534346};
DE            EC=1.-.-.- {ECO:0000269|PubMed:23978092};
DE   AltName: Full=Griseofulvin synthesis protein E {ECO:0000303|PubMed:20534346};
GN   Name=gsfE {ECO:0000303|PubMed:20534346};
OS   Penicillium aethiopicum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX   NCBI_TaxID=36650;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=IBT 5753;
RX   PubMed=20534346; DOI=10.1016/j.chembiol.2010.03.015;
RA   Chooi Y.H., Cacho R., Tang Y.;
RT   "Identification of the viridicatumtoxin and griseofulvin gene clusters from
RT   Penicillium aethiopicum.";
RL   Chem. Biol. 17:483-494(2010).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=13577889; DOI=10.1038/182476a0;
RA   Gentles J.C.;
RT   "Experimental ringworm in guinea pigs: oral treatment with griseofulvin.";
RL   Nature 182:476-477(1958).
RN   [3]
RP   BIOTECHNOLOGY.
RX   PubMed=4583105; DOI=10.1038/244292a0;
RA   Gull K., Trinci A.P.;
RT   "Griseofulvin inhibits fungal mitosis.";
RL   Nature 244:292-294(1973).
RN   [4]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND CATALYTIC ACTIVITY.
RX   PubMed=23978092; DOI=10.1021/cb400541z;
RA   Cacho R.A., Chooi Y.H., Zhou H., Tang Y.;
RT   "Complexity generation in fungal polyketide biosynthesis: a spirocycle-
RT   forming P450 in the concise pathway to the antifungal drug griseofulvin.";
RL   ACS Chem. Biol. 8:2322-2330(2013).
CC   -!- FUNCTION: Short chain dehydrogenase; part of the gene cluster that
CC       mediates the biosynthesis of griseofulvin, an important antifungal drug
CC       that has been in use for a long time for treating dermatophyte
CC       infections (PubMed:20534346, PubMed:23978092). The first step of the
CC       pathway is the formation of the heptaketide backbone by gsfA which is
CC       initiated by priming with acetyl-CoA, followed by sequential
CC       condensations of 6 malonyl-CoA units (PubMed:20534346). O-methylation
CC       at 3-OH by gsfB leads to griseophenone D which is further methylated at
CC       9-OH by gsfC to yield griseophenone C (PubMed:23978092). Griseophenone
CC       C is then substrate of halogenase gsfI which is responsible for the
CC       regio-specific chlorination at the C13 position to form griseophenone B
CC       (PubMed:23978092). The cytochrome P450 gsfF catalyzes the coupling of
CC       orcinol and phloroglucinol rings in griseophenone B to form desmethyl-
CC       dehydrogriseofulvin A which is further methylated at 5-OH by gsfD to
CC       yield dehydrogriseofulvin (PubMed:23978092). Finally, gsfE performs
CC       stereospecific reduction of enone 18 of dehydrogriseofulvin to afford
CC       the final product griseofulvin (PubMed:23978092).
CC       {ECO:0000269|PubMed:20534346, ECO:0000269|PubMed:23978092}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000269|PubMed:20534346, ECO:0000269|PubMed:23978092}.
CC   -!- DISRUPTION PHENOTYPE: Impairs the production of griseofulvin, but
CC       accumulates the intermediates dehydrogriseofulvin and dechloro-
CC       dehydrogriseofulvi (PubMed:23978092). {ECO:0000269|PubMed:23978092}.
CC   -!- BIOTECHNOLOGY: Griseofulvin is a spirocyclic fungal natural product
CC       used in treatment of fungal dermatophytes (PubMed:13577889,
CC       PubMed:4583105). {ECO:0000269|PubMed:13577889,
CC       ECO:0000269|PubMed:4583105}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. Highly divergent. {ECO:0000305}.
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DR   EMBL; GU574478; ADI24957.1; -; Genomic_DNA.
DR   AlphaFoldDB; D7PI19; -.
DR   SMR; D7PI19; -.
DR   BioCyc; MetaCyc:MON-19273; -.
DR   UniPathway; UPA00213; -.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   NADP; Nucleotide-binding; Oxidoreductase.
FT   CHAIN           1..377
FT                   /note="Short chain dehydrogenase gsfE"
FT                   /id="PRO_0000436724"
FT   ACT_SITE        125
FT                   /evidence="ECO:0000250|UniProtKB:Q6PQJ9"
FT   ACT_SITE        153
FT                   /evidence="ECO:0000250|UniProtKB:Q6PQJ9"
FT   BINDING         11..13
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PQJ9"
FT   BINDING         39..40
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PQJ9"
FT   BINDING         57..58
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PQJ9"
FT   BINDING         121
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PQJ9"
FT   BINDING         179
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PQJ9"
FT   BINDING         187..189
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PQJ9"
SQ   SEQUENCE   377 AA;  42538 MW;  74030079D4B61250 CRC64;
     MPKTAFITGA NGLSGSAIVE YLCNTTTSDD WGSIIVTSRS PFKSTVMDPR IKFIALDFVN
     DVSSLVETMK EVCGAVTHAY FCSYLHKDDF AESYTVNKAL FENFIAAIDK AAPKLENVTL
     QTGGKYYNLH VEPVPSPARE NDPRRYGPFE NFYFTQEDTL AEMQRGKTWS WNVIRPEAII
     GANSQPYGLN VALTIAMYFL ICRELGSASP MPTNQRYWEG TDDVSYAPLI ADLTIFVSTR
     KSCANEAFNV TNGDYFTWRY MWPRLAASLG AKADSQQCFE KPMPGEGELQ LDWSLAEWCK
     DKRKVWEDLC DRQGLPGAKA TFDLAGWAVG DFLYQRTWSA TLSVNKARRF GWTGHMDSYQ
     SFVDTFDKFR QLGLIPK
 
 
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