ID   GSFJ_PENAE              Reviewed;         554 AA.
AC   D7PI13;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   10-AUG-2010, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Probable efflux pump gsfJ {ECO:0000303|PubMed:20534346};
DE   AltName: Full=Griseofulvin synthesis protein J {ECO:0000303|PubMed:20534346};
GN   Name=gsfJ {ECO:0000303|PubMed:20534346};
OS   Penicillium aethiopicum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX   NCBI_TaxID=36650;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=IBT 5753;
RX   PubMed=20534346; DOI=10.1016/j.chembiol.2010.03.015;
RA   Chooi Y.H., Cacho R., Tang Y.;
RT   "Identification of the viridicatumtoxin and griseofulvin gene clusters from
RT   Penicillium aethiopicum.";
RL   Chem. Biol. 17:483-494(2010).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=13577889; DOI=10.1038/182476a0;
RA   Gentles J.C.;
RT   "Experimental ringworm in guinea pigs: oral treatment with griseofulvin.";
RL   Nature 182:476-477(1958).
RN   [3]
RP   BIOTECHNOLOGY.
RX   PubMed=4583105; DOI=10.1038/244292a0;
RA   Gull K., Trinci A.P.;
RT   "Griseofulvin inhibits fungal mitosis.";
RL   Nature 244:292-294(1973).
RN   [4]
RP   FUNCTION.
RX   PubMed=23978092; DOI=10.1021/cb400541z;
RA   Cacho R.A., Chooi Y.H., Zhou H., Tang Y.;
RT   "Complexity generation in fungal polyketide biosynthesis: a spirocycle-
RT   forming P450 in the concise pathway to the antifungal drug griseofulvin.";
RL   ACS Chem. Biol. 8:2322-2330(2013).
CC   -!- FUNCTION: Probable efflux pump; part of the gene cluster that mediates
CC       the biosynthesis of griseofulvin. {ECO:0000269|PubMed:20534346,
CC       ECO:0000269|PubMed:23978092}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- BIOTECHNOLOGY: Griseofulvin is a spirocyclic fungal natural product
CC       used in treatment of fungal dermatophytes (PubMed:13577889,
CC       PubMed:4583105). {ECO:0000269|PubMed:13577889,
CC       ECO:0000269|PubMed:4583105}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; GU574478; ADI24949.1; -; Genomic_DNA.
DR   AlphaFoldDB; D7PI13; -.
DR   SMR; D7PI13; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 2.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..554
FT                   /note="Probable efflux pump gsfJ"
FT                   /id="PRO_0000436729"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        181..201
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        206..226
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        248..268
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        279..299
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        321..341
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        349..369
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        379..399
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        410..430
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        447..467
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        518..538
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   554 AA;  59587 MW;  8E8367420E234816 CRC64;
     MDKQNTDSEK RATAEAPPQS LCTLVSSSEQ GGMDITNHNA KAGAADEYPH GVRLAAVVFS
     LMLGMFLVAL DNTILGTAIP KITDEFHDLN KVSWYGSAYL MTFGCGFQST WGKFYKYFPI
     KVWFLVAVFI FEVGSLICAV AQNPTTLIVG RAIAGFGGSG VGVGIFTIIG FAAPPENRPQ
     LLGFTGATYG IAAVLGPLIG GAFTDKCFYI NLPIGGVAAG TIFLLFKPPT SASPAKATPK
     EKFLQMDLVG ATLMMGLIVS YILALQYGGQ THSWKSSEVI GLLVGFFLFV LAFVTWEIYQ
     KERAMIVPRL FMRRYISVGS IYMFFFSGAY FIILYYLPIY FQSVYNSSPI GSGVKMLALI
     IPLTLAAIVQ GWALSKIRIV PLFWIIGGAL GTVGCGLFYT FDTETSVGKW VGYQIIVGFS
     TGWTFQIAMS NAQVHAPPED MSQATAIVNF FMTVGGAFFI SAAQCAFSNQ LIKTITKNLP
     ELDPTVAIST GATQIREAFT ASQVPIVVDA YMVGLKAVFA ITIAAFGVAT VIGFFGSWKK
     LLADELEKAT GGVA