GSG1_MOUSE
ID GSG1_MOUSE Reviewed; 324 AA.
AC Q8R1W2; Q8C2N5; Q9D9Z3; Q9Z1H7;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Germ cell-specific gene 1 protein;
DE AltName: Full=Germ cell-associated protein 1;
GN Name=Gsg1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=7957958; DOI=10.1016/0014-5793(94)01155-9;
RA Tanaka H., Yoshimura Y., Nishina Y., Nozaki M., Nojima H., Nishimune Y.;
RT "Isolation and characterization of cDNA clones specifically expressed in
RT testicular germ cells.";
RL FEBS Lett. 355:4-10(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=9337410; DOI=10.1007/s003359900602;
RA Matsui M., Ichihara H., Kobayashi S., Tanaka H., Tsuchida J., Nozaki M.,
RA Yoshimura Y., Nojima H., Rochelle J.M., Nishimune Y., Taketo M.M.,
RA Seldin M.F.;
RT "Mapping of six germ-cell-specific genes to mouse chromosomes.";
RL Mamm. Genome 8:873-874(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Testis, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP FUNCTION, INTERACTION WITH PAPOLB, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=18325338; DOI=10.1016/j.febslet.2008.01.065;
RA Choi H.-S., Lee S.-H., Kim H., Lee Y.;
RT "Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to
RT the endoplasmic reticulum through protein-protein interactions.";
RL FEBS Lett. 582:1203-1209(2008).
CC -!- FUNCTION: May cause the redistribution of PAPOLB from the cytosol to
CC the endoplasmic reticulum. {ECO:0000269|PubMed:18325338}.
CC -!- SUBUNIT: Interacts with PAPOLB. {ECO:0000269|PubMed:18325338}.
CC -!- INTERACTION:
CC Q8R1W2; Q9WVP6: Papolb; NbExp=8; IntAct=EBI-7842142, EBI-7842113;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:18325338}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:18325338}. Note=Colocalizes with PAPOLB in the
CC endoplasmic reticulum.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q8R1W2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8R1W2-2; Sequence=VSP_033001;
CC Name=3;
CC IsoId=Q8R1W2-3; Sequence=VSP_032999, VSP_033000;
CC Name=4;
CC IsoId=Q8R1W2-4; Sequence=VSP_032998, VSP_033001;
CC -!- TISSUE SPECIFICITY: Expressed in spermatogenic cells (at protein
CC level). Expressed in germ cells within the testis from day 21 onwards.
CC {ECO:0000269|PubMed:18325338, ECO:0000269|PubMed:7957958}.
CC -!- SIMILARITY: Belongs to the GSG1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH23009.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAA37087.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; D87325; BAA37087.1; ALT_FRAME; mRNA.
DR EMBL; AK006326; BAB24527.1; -; mRNA.
DR EMBL; AK088285; BAC40258.1; -; mRNA.
DR EMBL; AC122820; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC023009; AAH23009.1; ALT_INIT; mRNA.
DR CCDS; CCDS39682.1; -. [Q8R1W2-2]
DR CCDS; CCDS39683.1; -. [Q8R1W2-4]
DR RefSeq; NP_001074021.1; NM_001080552.1. [Q8R1W2-2]
DR RefSeq; NP_001074022.1; NM_001080553.1. [Q8R1W2-4]
DR RefSeq; NP_034482.2; NM_010352.2. [Q8R1W2-4]
DR AlphaFoldDB; Q8R1W2; -.
DR SMR; Q8R1W2; -.
DR BioGRID; 200083; 1.
DR IntAct; Q8R1W2; 3.
DR MINT; Q8R1W2; -.
DR iPTMnet; Q8R1W2; -.
DR PhosphoSitePlus; Q8R1W2; -.
DR PRIDE; Q8R1W2; -.
DR Antibodypedia; 23587; 131 antibodies from 18 providers.
DR DNASU; 14840; -.
DR Ensembl; ENSMUST00000087729; ENSMUSP00000085022; ENSMUSG00000030206. [Q8R1W2-2]
DR Ensembl; ENSMUST00000111910; ENSMUSP00000107541; ENSMUSG00000030206. [Q8R1W2-4]
DR Ensembl; ENSMUST00000111911; ENSMUSP00000107542; ENSMUSG00000030206. [Q8R1W2-4]
DR GeneID; 14840; -.
DR KEGG; mmu:14840; -.
DR UCSC; uc009elj.1; mouse. [Q8R1W2-2]
DR UCSC; uc009elk.1; mouse. [Q8R1W2-4]
DR CTD; 83445; -.
DR MGI; MGI:1194499; Gsg1.
DR VEuPathDB; HostDB:ENSMUSG00000030206; -.
DR GeneTree; ENSGT01050000244814; -.
DR InParanoid; Q8R1W2; -.
DR OMA; GAQITYI; -.
DR OrthoDB; 957556at2759; -.
DR PhylomeDB; Q8R1W2; -.
DR TreeFam; TF331388; -.
DR BioGRID-ORCS; 14840; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Gsg1; mouse.
DR PRO; PR:Q8R1W2; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8R1W2; protein.
DR Bgee; ENSMUSG00000030206; Expressed in seminiferous tubule of testis and 34 other tissues.
DR ExpressionAtlas; Q8R1W2; baseline and differential.
DR Genevisible; Q8R1W2; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0070063; F:RNA polymerase binding; IPI:MGI.
DR InterPro; IPR012478; GSG-1.
DR Pfam; PF07803; GSG-1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Endoplasmic reticulum; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..324
FT /note="Germ cell-specific gene 1 protein"
FT /id="PRO_0000329462"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..107
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032999"
FT VAR_SEQ 1..4
FT /note="MAKM -> M (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:7957958,
FT ECO:0000303|PubMed:9337410"
FT /id="VSP_032998"
FT VAR_SEQ 108..125
FT /note="EPGEKCRRFIELTPPAQR -> MEKASLLHLPWGPVAKVF (in isoform
FT 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_033000"
FT VAR_SEQ 125
FT /note="R -> RGEKGLLEFATLQGSCHPTLRFGGEWLMEKASLLHLPWGPVAKVF
FT (in isoform 2 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:7957958, ECO:0000303|PubMed:9337410"
FT /id="VSP_033001"
FT CONFLICT 178
FT /note="M -> I (in Ref. 3; BAC40258)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 324 AA; 36108 MW; 9AB3A42C147F2B90 CRC64;
MAKMEFQKGS SDQRTFISAI LNMLSLGLST ASLLSSEWFV GTQKVPKPLC GQSLAAKCFD
MPMSLDGGIA NTSAQEVVQY TWETGDDRFS FLAFRSGMWL SCEETMEEPG EKCRRFIELT
PPAQRWLSLG AQTAYIGLQL ISFLLLLTDL LLTTNPGCGL KLSAFAAVSL VLSGLLGMVA
HMLYSQVFQA TANLGPEDWR PHSWNYGWAF YTAWVSFTCC MASAVTTFNM YTRMVLEFKC
RHSKSFNTNP SCLAQHHRCF LPPPLTCTTH AGEPLSSCHQ YPSHPIRSVS EAIDLYSALQ
DKEFQQGISQ ELKEVVEPSV EEQR
