GSH0_SCHPO
ID GSH0_SCHPO Reviewed; 287 AA.
AC O94246;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Putative glutamate--cysteine ligase regulatory subunit;
DE AltName: Full=GCS light chain;
DE AltName: Full=Gamma-ECS regulatory subunit;
DE AltName: Full=Gamma-glutamylcysteine synthetase regulatory subunit;
DE AltName: Full=Glutamate--cysteine ligase modifier subunit;
GN ORFNames=SPCC737.06c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from
CC L-cysteine and L-glutamate: step 1/2.
CC -!- SUBUNIT: Heterodimer of a catalytic heavy chain and a regulatory light
CC chain. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the aldo/keto reductase family. Glutamate--
CC cysteine ligase light chain subfamily. {ECO:0000305}.
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DR EMBL; CU329672; CAA20862.1; -; Genomic_DNA.
DR PIR; T41579; T41579.
DR RefSeq; NP_588368.1; NM_001023359.2.
DR AlphaFoldDB; O94246; -.
DR SMR; O94246; -.
DR BioGRID; 275632; 24.
DR STRING; 4896.SPCC737.06c.1; -.
DR iPTMnet; O94246; -.
DR MaxQB; O94246; -.
DR PaxDb; O94246; -.
DR PRIDE; O94246; -.
DR EnsemblFungi; SPCC737.06c.1; SPCC737.06c.1:pep; SPCC737.06c.
DR GeneID; 2539059; -.
DR KEGG; spo:SPCC737.06c; -.
DR PomBase; SPCC737.06c; -.
DR VEuPathDB; FungiDB:SPCC737.06c; -.
DR eggNOG; KOG3023; Eukaryota.
DR HOGENOM; CLU_973728_0_0_1; -.
DR InParanoid; O94246; -.
DR OMA; NWGCLRK; -.
DR PhylomeDB; O94246; -.
DR Reactome; R-SPO-174403; Glutathione synthesis and recycling.
DR UniPathway; UPA00142; UER00209.
DR PRO; PR:O94246; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0017109; C:glutamate-cysteine ligase complex; ISO:PomBase.
DR GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central.
DR GO; GO:0035226; F:glutamate-cysteine ligase catalytic subunit binding; ISO:PomBase.
DR GO; GO:1990609; F:glutamate-cysteine ligase regulator activity; ISO:PomBase.
DR GO; GO:1990748; P:cellular detoxification; NAS:PomBase.
DR GO; GO:0006534; P:cysteine metabolic process; IC:PomBase.
DR GO; GO:0006750; P:glutathione biosynthetic process; ISO:PomBase.
DR GO; GO:0035229; P:positive regulation of glutamate-cysteine ligase activity; IBA:GO_Central.
DR Gene3D; 3.20.20.100; -; 1.
DR InterPro; IPR032963; Gclm.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR13295; PTHR13295; 1.
DR SUPFAM; SSF51430; SSF51430; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Glutathione biosynthesis; Reference proteome.
FT CHAIN 1..287
FT /note="Putative glutamate--cysteine ligase regulatory
FT subunit"
FT /id="PRO_0000310836"
SQ SEQUENCE 287 AA; 32660 MW; AEF98D8DE0C5C13C CRC64;
MIQNEKKHLI LYTGDVTKNL KSGIWNASRI KSNLELVKAL ENVKLTKFTG DGDENLKKNI
KVLVPVNEKP QKLDGKQEEY EIIVKLFFLD GENIDIKKRE ETLSQVFYNL HMLFGIDFVS
TLVVSFPHIT FLKESGNSSS NEIYDSIDEI PPQEIQSWVD TWKLLEEKVG EGKIGTLGVS
EFGVNELQRL ISSVNVVPES TQINIGQNCK LPNDLLNFAD RHHLKLFFHS DPSALLSESE
ITSVIHKACP EIPNPARVDW VIRYTILTRH TAVIHQKGYI VQSTYTE
