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GSH1A_ORYSI
ID   GSH1A_ORYSI             Reviewed;         492 AA.
AC   Q8GU95; A2XZZ8; B8AXK6; Q8GU96;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Glutamate--cysteine ligase A, chloroplastic;
DE            EC=6.3.2.2;
DE   AltName: Full=Gamma-ECS A;
DE            Short=GCS A;
DE   AltName: Full=Gamma-glutamylcysteine synthetase A;
DE   Flags: Precursor;
GN   Name=GSH1-1; ORFNames=OsI_18304;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RA   Peng L.T.;
RT   "The functional analysis of gamma-glutamylcysteine synthetase gene in Oryza
RT   sativa.";
RL   Thesis (2003), Institute of Plant Physiology and Ecology, China.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-
CC         cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from
CC       L-cysteine and L-glutamate: step 1/2.
CC   -!- SUBUNIT: Homodimer or monomer when oxidized or reduced, respectively.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC   -!- PTM: The Cys-156-Cys-376 disulfide bridge is known to modulate the
CC       enzyme activity according to the redox status. The oxidized form
CC       constitutes the active enzyme (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the carboxylate-amine ligase family. Glutamate--
CC       cysteine ligase type 2 subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAD48598.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AJ508915; CAD48598.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AJ508916; CAD48599.3; -; mRNA.
DR   EMBL; CM000130; EEC78447.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8GU95; -.
DR   SMR; Q8GU95; -.
DR   STRING; 39946.Q8GU95; -.
DR   EnsemblPlants; BGIOSGA019114-TA; BGIOSGA019114-PA; BGIOSGA019114.
DR   Gramene; BGIOSGA019114-TA; BGIOSGA019114-PA; BGIOSGA019114.
DR   HOGENOM; CLU_026610_0_0_1; -.
DR   OMA; DYVEWAL; -.
DR   UniPathway; UPA00142; UER00209.
DR   Proteomes; UP000007015; Chromosome 5.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006750; P:glutathione biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR035434; GCL_bact_plant.
DR   InterPro; IPR006336; GCS2.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR011556; Glut_cys_lig_pln_type.
DR   PANTHER; PTHR34378; PTHR34378; 1.
DR   Pfam; PF04107; GCS2; 1.
DR   PIRSF; PIRSF017901; GCL; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   TIGRFAMs; TIGR01436; glu_cys_lig_pln; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Chloroplast; Disulfide bond; Glutathione biosynthesis; Ligase;
KW   Nucleotide-binding; Plastid; Reference proteome; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..492
FT                   /note="Glutamate--cysteine ligase A, chloroplastic"
FT                   /id="PRO_0000333021"
FT   DISULFID        156..376
FT                   /evidence="ECO:0000250"
FT   CONFLICT        240
FT                   /note="H -> R (in Ref. 1; CAD48598/CAD48599)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   492 AA;  55662 MW;  F13E5F2B9F05A9A0 CRC64;
     MAVASRLAVA RVAPDGGAAG RRRRRGRPVV AVPTAGRGRG GAVAASPPTE EAVQMTEPLT
     KEDLVAYLVS GCKPKENWRI GTEHEKFGFE VDTLRPIKYD QIRDILNGLA ERFDWDKIVE
     ENNVIGLKQG KQSISLEPGG QFELSGAPLE TLHQTCAEVN SHLYQVKAVG EEMGIGFLGI
     GFQPKWALSD IPIMPKGRYE IMRNYMPKVG SLGLDMMFRT CTVQVNLDFS SEQDMIRKFH
     TGLALQPIAT AIFANSPFKE GKPNGYLSLR SHIWTDTDNN RSGMLPFVFD DSFGFERYVD
     YALDVPMYFV YRNKKYIDCT GMSFRDFMVG KLPQAPGELP TLNDWENHLT TIFPEVRLKR
     YLEMRGADGG PWRRLCALPA FWVGLLYDEE SLQSISDMTS DWTNEEREML RRKVPVTGLK
     TPFRDGYVRD LAEEILQLSK NGLERRGYKE VGFLREVDAV ISSGVTPAER LLNLYETKWQ
     RSVDPVFQEL LY
 
 
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