GSH1A_ORYSJ
ID GSH1A_ORYSJ Reviewed; 492 AA.
AC Q688Q9; B7EUG4;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Glutamate--cysteine ligase A, chloroplastic;
DE EC=6.3.2.2;
DE AltName: Full=Gamma-ECS A;
DE Short=GCS A;
DE AltName: Full=Gamma-glutamylcysteine synthetase A;
DE Flags: Precursor;
GN Name=GSH1-1; OrderedLocusNames=Os05g0129000, LOC_Os05g03820;
GN ORFNames=OSJNBa0056I11.11;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-
CC cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
CC -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from
CC L-cysteine and L-glutamate: step 1/2.
CC -!- SUBUNIT: Homodimer or monomer when oxidized or reduced, respectively.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC -!- PTM: The Cys-156-Cys-376 disulfide bridge is known to modulate the
CC enzyme activity according to the redox status. The oxidized form
CC constitutes the active enzyme (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the carboxylate-amine ligase family. Glutamate--
CC cysteine ligase type 2 subfamily. {ECO:0000305}.
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DR EMBL; AC130598; AAU10747.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF16455.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS92097.1; -; Genomic_DNA.
DR EMBL; AK103315; BAG96011.1; -; mRNA.
DR RefSeq; XP_015640661.1; XM_015785175.1.
DR RefSeq; XP_015640662.1; XM_015785176.1.
DR AlphaFoldDB; Q688Q9; -.
DR SMR; Q688Q9; -.
DR STRING; 4530.OS05T0129000-01; -.
DR PaxDb; Q688Q9; -.
DR PRIDE; Q688Q9; -.
DR EnsemblPlants; Os05t0129000-01; Os05t0129000-01; Os05g0129000.
DR GeneID; 4337696; -.
DR Gramene; Os05t0129000-01; Os05t0129000-01; Os05g0129000.
DR KEGG; osa:4337696; -.
DR eggNOG; ENOG502QVEN; Eukaryota.
DR HOGENOM; CLU_026610_0_0_1; -.
DR InParanoid; Q688Q9; -.
DR OMA; DYVEWAL; -.
DR OrthoDB; 397529at2759; -.
DR PlantReactome; R-OSA-1119342; Gamma-glutamyl cycle.
DR PlantReactome; R-OSA-1119483; Glutathione biosynthesis.
DR UniPathway; UPA00142; UER00209.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR ExpressionAtlas; Q688Q9; baseline and differential.
DR Genevisible; Q688Q9; OS.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006750; P:glutathione biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR035434; GCL_bact_plant.
DR InterPro; IPR006336; GCS2.
DR InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR InterPro; IPR011556; Glut_cys_lig_pln_type.
DR PANTHER; PTHR34378; PTHR34378; 1.
DR Pfam; PF04107; GCS2; 1.
DR PIRSF; PIRSF017901; GCL; 1.
DR SUPFAM; SSF55931; SSF55931; 1.
DR TIGRFAMs; TIGR01436; glu_cys_lig_pln; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chloroplast; Disulfide bond; Glutathione biosynthesis; Ligase;
KW Nucleotide-binding; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN ?..492
FT /note="Glutamate--cysteine ligase A, chloroplastic"
FT /id="PRO_0000333022"
FT DISULFID 156..376
FT /evidence="ECO:0000250"
SQ SEQUENCE 492 AA; 55723 MW; 4798C6DBA0215FAF CRC64;
MAVASRLAVA RVAPDGGAAG RRRRRGRPVV AVPTAGRGRG GAVAASPPTE EAVQMTEPLT
KEDLVAYLVS GCKPKENWRI GTEHEKFGFE VDTLRPIKYD QIRDILNGLA ERFDWDKIVE
ENNVIGLKQG KQSISLEPGG QFELSGAPLE TLHQTCAEVN SHLYQVKAVG EEMGIGFLGI
GFQPKWALSD IPIMPKGRYE IMRNYMPKVG SLGLDMMFRT CTVQVNLDFS SEQDMIRKFR
TGLALQPIAT AIFANSPFKE GKPNGYLSLR SHIWTDTDNN RSGMLPFVFD DSFGFERYVD
YALDIPMYFV YRNKKYIDCT GMSFRDFMVG KLPQAPGELP TLNDWENHLT TIFPEVRLKR
YLEMRGADGG PWRRLCALPV FWVGLLYDEE SLQSISDMTS DWTNEEREML RRKVPVTGLK
TPFRDGYVRD LAEEILQLSK NGLERRGYKE VGFLREVDAV ISSGVTPAER LLNLYETKWQ
RSVDPVFQEL LY
