GSH1_KLEP7
ID GSH1_KLEP7 Reviewed; 518 AA.
AC A6TCV4;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=Glutamate--cysteine ligase {ECO:0000255|HAMAP-Rule:MF_00578};
DE EC=6.3.2.2 {ECO:0000255|HAMAP-Rule:MF_00578};
DE AltName: Full=Gamma-ECS {ECO:0000255|HAMAP-Rule:MF_00578};
DE Short=GCS {ECO:0000255|HAMAP-Rule:MF_00578};
DE AltName: Full=Gamma-glutamylcysteine synthetase {ECO:0000255|HAMAP-Rule:MF_00578};
GN Name=gshA {ECO:0000255|HAMAP-Rule:MF_00578};
GN OrderedLocusNames=KPN78578_29640; ORFNames=KPN_03019;
OS Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=272620;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700721 / MGH 78578;
RG The Klebsiella pneumonia Genome Sequencing Project;
RA McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P.,
RA Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.;
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-
CC cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00578};
CC -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from
CC L-cysteine and L-glutamate: step 1/2. {ECO:0000255|HAMAP-
CC Rule:MF_00578}.
CC -!- SIMILARITY: Belongs to the glutamate--cysteine ligase type 1 family.
CC Type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_00578}.
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DR EMBL; CP000647; ABR78425.1; -; Genomic_DNA.
DR RefSeq; WP_002914353.1; NC_009648.1.
DR AlphaFoldDB; A6TCV4; -.
DR SMR; A6TCV4; -.
DR STRING; 272620.KPN_03019; -.
DR EnsemblBacteria; ABR78425; ABR78425; KPN_03019.
DR KEGG; kpn:KPN_03019; -.
DR HOGENOM; CLU_020728_3_0_6; -.
DR OMA; RYSWLLM; -.
DR UniPathway; UPA00142; UER00209.
DR Proteomes; UP000000265; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006750; P:glutathione biosynthetic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00578; Glu_cys_ligase; 1.
DR InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR InterPro; IPR007370; Glu_cys_ligase.
DR InterPro; IPR006334; Glut_cys_ligase.
DR PANTHER; PTHR38761; PTHR38761; 1.
DR Pfam; PF04262; Glu_cys_ligase; 1.
DR SUPFAM; SSF55931; SSF55931; 1.
DR TIGRFAMs; TIGR01434; glu_cys_ligase; 1.
PE 3: Inferred from homology;
KW ATP-binding; Glutathione biosynthesis; Ligase; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..518
FT /note="Glutamate--cysteine ligase"
FT /id="PRO_1000025175"
SQ SEQUENCE 518 AA; 58101 MW; 80276982F050682D CRC64;
MIPDVSQALA WLEKHPQALQ GIQRGLERET LRVNADGTLA TTGHPPALGS ALTHKWITTD
FAEALLEFIT PVDGDIEHML TFMRDVHRYT ARQLGDERMW PLSMPCYIAP GQDIELAQYG
TSNVGRLKTL YREGLKNRYG ALMQTISGVH YNFSLPMAFW QAKCGVEDAE SGKEAISAGY
FRSIRNYYRF GWVIPYLFGA SPAICSSFLQ GKPTTLPFEE TGNGMYYLPY ATSLRLSDLG
YTNKSQSNLG ITFNDLHEYV AGLKRAIKTP SEEYAKIGLQ KDGKYLQINS NILQIENELY
APIRPKRVTR RGETPSDALL RGGIEYIEVR SLDINPFSPI GVDAQQVRFL DLFMVWCALA
DAPEMSSDEL LCTRTNWNRV ILEGRKPGLT LGIGCESAQF PLAQVGKDLF RDLRRVAQTL
DSIHGGQAYQ QVCDELLACF DDPELTFSAR ILRSMIEEGI GGTGRALADR YRTQLREEPL
EILSEDDFIA ERDASVARQK KVEAEDSEPF EALLARHA
