ID   GSH1_LEPIN              Reviewed;         520 AA.
AC   Q8F4D5;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2005, sequence version 2.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Glutamate--cysteine ligase {ECO:0000255|HAMAP-Rule:MF_00578};
DE            EC=6.3.2.2 {ECO:0000255|HAMAP-Rule:MF_00578};
DE   AltName: Full=Gamma-ECS {ECO:0000255|HAMAP-Rule:MF_00578};
DE            Short=GCS {ECO:0000255|HAMAP-Rule:MF_00578};
DE   AltName: Full=Gamma-glutamylcysteine synthetase {ECO:0000255|HAMAP-Rule:MF_00578};
GN   Name=gshA {ECO:0000255|HAMAP-Rule:MF_00578}; OrderedLocusNames=LA_2106;
OS   Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain
OS   56601).
OC   Bacteria; Spirochaetes; Leptospirales; Leptospiraceae; Leptospira.
OX   NCBI_TaxID=189518;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=56601;
RX   PubMed=12712204; DOI=10.1038/nature01597;
RA   Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X.,
RA   Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X.,
RA   Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H., Yin H.-F., Zhang Y., Zhu G.-F.,
RA   Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y.,
RA   Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I.,
RA   Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P.;
RT   "Unique physiological and pathogenic features of Leptospira interrogans
RT   revealed by whole-genome sequencing.";
RL   Nature 422:888-893(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-
CC         cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00578};
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from
CC       L-cysteine and L-glutamate: step 1/2. {ECO:0000255|HAMAP-
CC       Rule:MF_00578}.
CC   -!- SIMILARITY: Belongs to the glutamate--cysteine ligase type 1 family.
CC       Type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_00578}.
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DR   EMBL; AE010300; AAN49305.2; -; Genomic_DNA.
DR   RefSeq; NP_712287.2; NC_004342.2.
DR   RefSeq; WP_000849965.1; NC_004342.2.
DR   AlphaFoldDB; Q8F4D5; -.
DR   SMR; Q8F4D5; -.
DR   STRING; 189518.LA_2106; -.
DR   EnsemblBacteria; AAN49305; AAN49305; LA_2106.
DR   KEGG; lil:LA_2106; -.
DR   PATRIC; fig|189518.3.peg.2100; -.
DR   HOGENOM; CLU_020728_3_0_12; -.
DR   InParanoid; Q8F4D5; -.
DR   OMA; RYSWLLM; -.
DR   UniPathway; UPA00142; UER00209.
DR   Proteomes; UP000001408; Chromosome I.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004357; F:glutamate-cysteine ligase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IBA:GO_Central.
DR   GO; GO:0006750; P:glutathione biosynthetic process; IBA:GO_Central.
DR   HAMAP; MF_00578; Glu_cys_ligase; 1.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR007370; Glu_cys_ligase.
DR   InterPro; IPR006334; Glut_cys_ligase.
DR   PANTHER; PTHR38761; PTHR38761; 1.
DR   Pfam; PF04262; Glu_cys_ligase; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   TIGRFAMs; TIGR01434; glu_cys_ligase; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Glutathione biosynthesis; Ligase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..520
FT                   /note="Glutamate--cysteine ligase"
FT                   /id="PRO_0000192529"
SQ   SEQUENCE   520 AA;  60577 MW;  16D408044D5C186D CRC64;
     MKTKELTQSK IEEVSLEILL RHAVKAKHGL EKESMRVNPD GTLSGTTHPI HLGSSLTNHY
     IKTDFAEPQL EYATHPRPKV EANIRELQDL HIFTIRKLEN ELIWPFSMPP VLPEEENEIP
     LGQYGTSHSG RWKTIYRHGL GLRYGRRMQT ISGVHYNFSF SKVFLRQFLG KEISNFTKEE
     ISSLYLHVIR NFLRRVHFLT YLTGSSPVFD FTFLPNPGSL KFEKHKNFTL YSTYATSLRM
     SEIGYTSKVQ DTLGIHYNSL EEYVDRMCYA VHTPYPKYVS FSENKDAQLN PNYLQIENEF
     YSPIRPKQVP KGDERPLDAL LQRGIEYIEI RSLDIDPYSP VGVCRSNLAF TQLILLDSLL
     KVSPSISEEE NFSLKENLNS VIWEGRNPEL KINVNGSKRN FQEAGAEYSE SLRHYAKILD
     LHTGRRTYQE AIDFQIKKWK NPDKTPSGKL LSEILKRNIE FREKGIELAQ ENKRMFSYLE
     YSPGTLMKME KETIRSFQEK EELEKQEIQT QYPTVKLCNH