ID   GSH1_ONCVO              Reviewed;         652 AA.
AC   Q9NFN6; O44933;
DT   30-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2002, sequence version 2.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Glutamate--cysteine ligase;
DE            EC=6.3.2.2;
DE   AltName: Full=Gamma-ECS;
DE            Short=GCS;
DE   AltName: Full=Gamma-glutamylcysteine synthetase;
GN   Name=gcs-1;
OS   Onchocerca volvulus.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Onchocerca.
OX   NCBI_TaxID=6282;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX   PubMed=11163433; DOI=10.1016/s0166-6851(00)00325-x;
RA   Luersen K., Mueller S., Hussein A., Liebau E., Walter R.D.;
RT   "The gamma-glutamylcysteine synthetase of Onchocerca volvulus.";
RL   Mol. Biochem. Parasitol. 111:243-251(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-
CC         cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from
CC       L-cysteine and L-glutamate: step 1/2.
CC   -!- SIMILARITY: Belongs to the glutamate--cysteine ligase type 3 family.
CC       {ECO:0000305}.
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DR   EMBL; AF042168; AAB96970.1; -; mRNA.
DR   EMBL; AJ276620; CAB82447.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9NFN6; -.
DR   SMR; Q9NFN6; -.
DR   STRING; 6282.Q9NFN6; -.
DR   BindingDB; Q9NFN6; -.
DR   ChEMBL; CHEMBL2366513; -.
DR   PRIDE; Q9NFN6; -.
DR   HOGENOM; CLU_010467_0_0_1; -.
DR   BRENDA; 6.3.2.2; 4401.
DR   UniPathway; UPA00142; UER00209.
DR   Proteomes; UP000024404; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006750; P:glutathione biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR004308; GCS.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   PANTHER; PTHR11164; PTHR11164; 1.
DR   Pfam; PF03074; GCS; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Glutathione biosynthesis; Ligase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..652
FT                   /note="Glutamate--cysteine ligase"
FT                   /id="PRO_0000192567"
FT   CONFLICT        24
FT                   /note="Missing (in Ref. 1; CAB82447)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        315
FT                   /note="T -> S (in Ref. 1; CAB82447)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   652 AA;  74212 MW;  F8BAFB591E76F03F CRC64;
     MGLLTLGTPL PWNETVPYVD YIKEHGIAQF IALYHRLKGR EGDQLKWGDE IEYTIVKFDD
     DAKRVRVSLR AEELLHQLQA GEELNALLGN ENCCLWRPEF ASYMIEGTPG APYGGLLACF
     NVVESNMISR RAEVTRLLEN GESIMSISFP ALGTPDFTSP PYEPRPDDIN SFGCSLFFPD
     EVIYGGHPRF RNLVRNIRQR RGEKVAINVP IYKDINTPSP YQEDFTKAKD GGQAARAAKS
     DHIYMDHMGF GMGCCCLQVT FQAVNIDEAR WLYDQLTPIT PVLLALSAAT PVFRSRLADV
     DSRWDVISAS VDDRTAEERG LVPLKNNKFV LEKSRYDTTD CYIYPCSESY NDIPLQYDDK
     IYKQLIDGGI DDLLAQHIAH MFIRDPLQVF RERIEQDDTK STEHFETVQS SNWMNMRFKP
     PPPDSEIGWR VEFRPSEVQL TDFENAAYCC FVVLLTRVMI SFRITLILPI SALTENMKRA
     QRRNAVLEQK LLFRKGIATC NSPPCARGAG CTLESDDVVE MTVNEIINGN GNDFPGLIPL
     MRQYLDSADV DVDSRCTVSQ YLSFIQKRAS GELQTLAAWM REFISEHPEY KHDSYVGDRI
     IYDMLKEMDR ISKGEISCPK LLGDYCTKTD SRIPMAVRRA EEKLIVSTKK QS