ID   GSH1_PSEAE              Reviewed;         527 AA.
AC   Q9HTY6;
DT   30-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Glutamate--cysteine ligase {ECO:0000255|HAMAP-Rule:MF_00578};
DE            EC=6.3.2.2 {ECO:0000255|HAMAP-Rule:MF_00578};
DE   AltName: Full=Gamma-ECS {ECO:0000255|HAMAP-Rule:MF_00578};
DE            Short=GCS {ECO:0000255|HAMAP-Rule:MF_00578};
DE   AltName: Full=Gamma-glutamylcysteine synthetase {ECO:0000255|HAMAP-Rule:MF_00578};
GN   Name=gshA {ECO:0000255|HAMAP-Rule:MF_00578}; OrderedLocusNames=PA5203;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-
CC         cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00578};
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from
CC       L-cysteine and L-glutamate: step 1/2. {ECO:0000255|HAMAP-
CC       Rule:MF_00578}.
CC   -!- SIMILARITY: Belongs to the glutamate--cysteine ligase type 1 family.
CC       Type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_00578}.
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DR   EMBL; AE004091; AAG08588.1; -; Genomic_DNA.
DR   PIR; F82995; F82995.
DR   RefSeq; NP_253890.1; NC_002516.2.
DR   RefSeq; WP_003109990.1; NZ_QZGE01000002.1.
DR   AlphaFoldDB; Q9HTY6; -.
DR   SMR; Q9HTY6; -.
DR   STRING; 287.DR97_2572; -.
DR   PaxDb; Q9HTY6; -.
DR   PRIDE; Q9HTY6; -.
DR   EnsemblBacteria; AAG08588; AAG08588; PA5203.
DR   GeneID; 879474; -.
DR   KEGG; pae:PA5203; -.
DR   PATRIC; fig|208964.12.peg.5453; -.
DR   PseudoCAP; PA5203; -.
DR   HOGENOM; CLU_020728_3_0_6; -.
DR   InParanoid; Q9HTY6; -.
DR   OMA; RYSWLLM; -.
DR   PhylomeDB; Q9HTY6; -.
DR   BioCyc; PAER208964:G1FZ6-5322-MON; -.
DR   UniPathway; UPA00142; UER00209.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004357; F:glutamate-cysteine ligase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IBA:GO_Central.
DR   GO; GO:0006750; P:glutathione biosynthetic process; IBA:GO_Central.
DR   HAMAP; MF_00578; Glu_cys_ligase; 1.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR007370; Glu_cys_ligase.
DR   InterPro; IPR006334; Glut_cys_ligase.
DR   PANTHER; PTHR38761; PTHR38761; 1.
DR   Pfam; PF04262; Glu_cys_ligase; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   TIGRFAMs; TIGR01434; glu_cys_ligase; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Glutathione biosynthesis; Ligase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..527
FT                   /note="Glutamate--cysteine ligase"
FT                   /id="PRO_0000192532"
SQ   SEQUENCE   527 AA;  59224 MW;  3765951CFB49A79E CRC64;
     MSDLLSRRLA LLGAAANLPL LTECLHGIER ECLRVDSDGK LALTPHPRAL GSTLTHPQIT
     TDYSEALLEF ITPTETDVAD TLGDLERIHR FASSKLDGEY LWSPSMPCEL PDEESIPIAR
     YGSSMIGRLK YVYRKGLALR YGKTMQCIAG IHYNFSLPER LWPLLRQAEG SELSERDYQS
     AAYIALIRNF RRYSWLLMYL FGASPALDAG FLRGRPSQLE RLDEHTLYLP YATSLRMSDL
     GYQNNAQAGL TPCYNDLQSY IDSLRQAVST PYPPYEKVGT KQDGEWVQLN TNILQIENEY
     YSSIRPKRVT YTGERPVQAL AARGVQYVEV RCLDINPFLP LGIDLDEARF LDAFLLFCAF
     SDSPLLNGEC SDATDNFLAV VKEGRRPGLQ LQRRGQPVEL QVWANELLER IADTAALLDR
     ARGGEAHAAA LAAQRAKVAD AELTPSAQVL KVMRERGESF EAFSLRQSRE HAEYFRQHPL
     AAEEQARFEK MASDSLAEQT ELERDQDGDF DTFVAAYQAS ILGLISN