ID   GSH1_SHEON              Reviewed;         523 AA.
AC   Q8EBF9;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 2.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Glutamate--cysteine ligase {ECO:0000255|HAMAP-Rule:MF_00578};
DE            EC=6.3.2.2 {ECO:0000255|HAMAP-Rule:MF_00578};
DE   AltName: Full=Gamma-ECS {ECO:0000255|HAMAP-Rule:MF_00578};
DE            Short=GCS {ECO:0000255|HAMAP-Rule:MF_00578};
DE   AltName: Full=Gamma-glutamylcysteine synthetase {ECO:0000255|HAMAP-Rule:MF_00578};
GN   Name=gshA {ECO:0000255|HAMAP-Rule:MF_00578}; OrderedLocusNames=SO_3559;
OS   Shewanella oneidensis (strain MR-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=211586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MR-1;
RX   PubMed=12368813; DOI=10.1038/nbt749;
RA   Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C.,
RA   Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A.,
RA   Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C.,
RA   DeBoy R.T., Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R.,
RA   Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J.,
RA   Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J.,
RA   Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V.,
RA   Smith H.O., Venter J.C., Nealson K.H., Fraser C.M.;
RT   "Genome sequence of the dissimilatory metal ion-reducing bacterium
RT   Shewanella oneidensis.";
RL   Nat. Biotechnol. 20:1118-1123(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-
CC         cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00578};
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from
CC       L-cysteine and L-glutamate: step 1/2. {ECO:0000255|HAMAP-
CC       Rule:MF_00578}.
CC   -!- SIMILARITY: Belongs to the glutamate--cysteine ligase type 1 family.
CC       Type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_00578}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN56550.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE014299; AAN56550.2; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_719106.2; NC_004347.2.
DR   AlphaFoldDB; Q8EBF9; -.
DR   SMR; Q8EBF9; -.
DR   STRING; 211586.SO_3559; -.
DR   PaxDb; Q8EBF9; -.
DR   KEGG; son:SO_3559; -.
DR   PATRIC; fig|211586.12.peg.3454; -.
DR   eggNOG; COG2918; Bacteria.
DR   HOGENOM; CLU_020728_3_0_6; -.
DR   OMA; RYSWLLM; -.
DR   OrthoDB; 967793at2; -.
DR   PhylomeDB; Q8EBF9; -.
DR   UniPathway; UPA00142; UER00209.
DR   Proteomes; UP000008186; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004357; F:glutamate-cysteine ligase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IBA:GO_Central.
DR   GO; GO:0006750; P:glutathione biosynthetic process; IBA:GO_Central.
DR   HAMAP; MF_00578; Glu_cys_ligase; 1.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR007370; Glu_cys_ligase.
DR   InterPro; IPR006334; Glut_cys_ligase.
DR   PANTHER; PTHR38761; PTHR38761; 1.
DR   Pfam; PF04262; Glu_cys_ligase; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   TIGRFAMs; TIGR01434; glu_cys_ligase; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Glutathione biosynthesis; Ligase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..523
FT                   /note="Glutamate--cysteine ligase"
FT                   /id="PRO_0000192540"
SQ   SEQUENCE   523 AA;  58378 MW;  F6B4726A5A40A69B CRC64;
     MKPFNELVQH FSDAQGRAAL LGMLRGIERE ALRIDESGYL ALDGHPLELG SALTHSRITT
     DYSEALLEFI TPVNHQVESL LQGLTETHAY SVRHLHGQRL WPVSMPCYVK DEANIPIARY
     GTSNTGKMKT LYRKGLTYRY GALMQIISGV HFNFSVSQEL WQSLYELSDK SLSFDDFISE
     SYFGLIRNYR RLVWVLPYLF GASPALCNSF IKGQKTDLRF EKSGRGTLYL PYATSLRMSD
     LGYTNKEQAD LNISYNSLPE YLAGIRAAIK MPSANFANIG VKVDGEYRQL NANVLQIENE
     FYSPIRAKRV TKSGEKPSEA LARAGVEYIE VRALDVNPFS PIGIEASQVR FLDLFLLYCL
     LTPSPKSDAA EEARLSANLK SVVLEGRKPG LELHTATGTL SLQTWLLELF DNLSSLAVLL
     DGETNAYQAA LAHWRDAVVD PQKTLSGQVL QQLVTKGQDH GQWVMSLAQQ YHQYFIDYPL
     SSEAASDYDA EAQSSLAKQV ELEAAQSAVS LDDYLTDYFG APA