ID   GSH1_VIBCH              Reviewed;         524 AA.
AC   Q9KUG5;
DT   30-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Glutamate--cysteine ligase {ECO:0000255|HAMAP-Rule:MF_00578};
DE            EC=6.3.2.2 {ECO:0000255|HAMAP-Rule:MF_00578};
DE   AltName: Full=Gamma-ECS {ECO:0000255|HAMAP-Rule:MF_00578};
DE            Short=GCS {ECO:0000255|HAMAP-Rule:MF_00578};
DE   AltName: Full=Gamma-glutamylcysteine synthetase {ECO:0000255|HAMAP-Rule:MF_00578};
GN   Name=gshA {ECO:0000255|HAMAP-Rule:MF_00578}; OrderedLocusNames=VC_0556;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-
CC         cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00578};
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from
CC       L-cysteine and L-glutamate: step 1/2. {ECO:0000255|HAMAP-
CC       Rule:MF_00578}.
CC   -!- SIMILARITY: Belongs to the glutamate--cysteine ligase type 1 family.
CC       Type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_00578}.
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DR   EMBL; AE003852; AAF93724.1; -; Genomic_DNA.
DR   PIR; E82309; E82309.
DR   RefSeq; NP_230207.1; NC_002505.1.
DR   RefSeq; WP_000032608.1; NZ_LT906614.1.
DR   AlphaFoldDB; Q9KUG5; -.
DR   SMR; Q9KUG5; -.
DR   STRING; 243277.VC_0556; -.
DR   PRIDE; Q9KUG5; -.
DR   DNASU; 2615233; -.
DR   EnsemblBacteria; AAF93724; AAF93724; VC_0556.
DR   GeneID; 57739279; -.
DR   KEGG; vch:VC_0556; -.
DR   PATRIC; fig|243277.26.peg.531; -.
DR   eggNOG; COG2918; Bacteria.
DR   HOGENOM; CLU_020728_3_0_6; -.
DR   OMA; RYSWLLM; -.
DR   BioCyc; VCHO:VC0556-MON; -.
DR   UniPathway; UPA00142; UER00209.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004357; F:glutamate-cysteine ligase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IBA:GO_Central.
DR   GO; GO:0006750; P:glutathione biosynthetic process; IBA:GO_Central.
DR   HAMAP; MF_00578; Glu_cys_ligase; 1.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR007370; Glu_cys_ligase.
DR   InterPro; IPR006334; Glut_cys_ligase.
DR   PANTHER; PTHR38761; PTHR38761; 1.
DR   Pfam; PF04262; Glu_cys_ligase; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   TIGRFAMs; TIGR01434; glu_cys_ligase; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Glutathione biosynthesis; Ligase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..524
FT                   /note="Glutamate--cysteine ligase"
FT                   /id="PRO_0000192541"
SQ   SEQUENCE   524 AA;  58961 MW;  1A4B4CEAEAA0D07B CRC64;
     MSKFAERLQT VANKPEVFQK FSRGLERESL RYTPEGALTQ TPHPKALGAA LTHRWITTDF
     AESLLEFITP VSHEVDTLLA QMSDIHHFAQ TKLGDEKLWP LSMPCYVGSE EGIVLAQYGS
     SNTGKMKTLY REGLKRRYGS LMQIISGVHF NFSFPESFWD ALFGEQEESA RQASKSAAYF
     GVIRNYYRFG WLIPYLFGAS PALCSSFLQG RKTDLPFESI GKTLYLPYAT SLRLSDLGYT
     NSAQSVLQIG FNSIEQYLQG LNEAIRTPSA EFAKLGVKVE GEYRQLNSNV LQIENELYAP
     IRPKRVTQSG ERPSQALARA GVEYIEVRSL DVNPFSPIGI NEDQVRFLDL FLAWTALSDS
     DPMDGCELAC WRDNWRKVVL EGRKPGLELQ IGCHGEVLTL QAWAKRVFAD LRVIAETMDA
     ALGGDAYQAV CAKLETWIEN PELTLSAQIL AEIKQHGGLG KTGCALGNEY RAVNLQHQYQ
     FYSQDEMEQE VRRSTQAQAD IEAKETLNFD AYLTQYFAYL QAVS