ID   GSHR_RAT                Reviewed;         424 AA.
AC   P70619;
DT   05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 2.
DT   25-MAY-2022, entry version 136.
DE   RecName: Full=Glutathione reductase;
DE            Short=GR;
DE            Short=GRase;
DE            EC=1.8.1.7;
DE   Flags: Fragment;
GN   Name=Gsr;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Hou W.M., Cheplinsky A.B.;
RL   Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Maintains high levels of reduced glutathione in the cytosol.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 glutathione + NADP(+) = glutathione disulfide + H(+) +
CC         NADPH; Xref=Rhea:RHEA:11740, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:57925, ChEBI:CHEBI:58297, ChEBI:CHEBI:58349; EC=1.8.1.7;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC       Note=Binds 1 FAD per subunit. {ECO:0000250};
CC   -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I pyridine nucleotide-disulfide
CC       oxidoreductase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB18132.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; U73174; AAB18132.1; ALT_FRAME; mRNA.
DR   AlphaFoldDB; P70619; -.
DR   SMR; P70619; -.
DR   STRING; 10116.ENSRNOP00000020252; -.
DR   BindingDB; P70619; -.
DR   ChEMBL; CHEMBL3286088; -.
DR   iPTMnet; P70619; -.
DR   PhosphoSitePlus; P70619; -.
DR   jPOST; P70619; -.
DR   PaxDb; P70619; -.
DR   PRIDE; P70619; -.
DR   UCSC; RGD:621747; rat.
DR   RGD; 621747; Gsr.
DR   eggNOG; KOG0405; Eukaryota.
DR   InParanoid; P70619; -.
DR   PhylomeDB; P70619; -.
DR   Reactome; R-RNO-3299685; Detoxification of Reactive Oxygen Species.
DR   Reactome; R-RNO-499943; Interconversion of nucleotide di- and triphosphates.
DR   Reactome; R-RNO-5628897; TP53 Regulates Metabolic Genes.
DR   SABIO-RK; P70619; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0005829; C:cytosol; IDA:RGD.
DR   GO; GO:0009897; C:external side of plasma membrane; ISO:RGD.
DR   GO; GO:0005739; C:mitochondrion; IDA:RGD.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IDA:RGD.
DR   GO; GO:0043295; F:glutathione binding; IDA:RGD.
DR   GO; GO:0004362; F:glutathione-disulfide reductase (NADPH) activity; IDA:CACAO.
DR   GO; GO:0042802; F:identical protein binding; IPI:RGD.
DR   GO; GO:0050661; F:NADP binding; IDA:RGD.
DR   GO; GO:0007568; P:aging; IEP:RGD.
DR   GO; GO:0045454; P:cell redox homeostasis; IBA:GO_Central.
DR   GO; GO:0034599; P:cellular response to oxidative stress; IBA:GO_Central.
DR   GO; GO:0006749; P:glutathione metabolic process; IDA:RGD.
DR   GO; GO:0014823; P:response to activity; IEP:RGD.
DR   GO; GO:0046686; P:response to cadmium ion; IEP:RGD.
DR   GO; GO:0045471; P:response to ethanol; IEP:RGD.
DR   GO; GO:0010041; P:response to iron(III) ion; IEP:RGD.
DR   GO; GO:0002931; P:response to ischemia; IEP:RGD.
DR   GO; GO:0035094; P:response to nicotine; IEP:RGD.
DR   GO; GO:0006979; P:response to oxidative stress; IEP:RGD.
DR   GO; GO:0033189; P:response to vitamin A; IEP:RGD.
DR   GO; GO:0007283; P:spermatogenesis; IMP:RGD.
DR   Gene3D; 3.30.390.30; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   InterPro; IPR016156; FAD/NAD-linked_Rdtase_dimer_sf.
DR   InterPro; IPR006322; Glutathione_Rdtase_euk/bac.
DR   InterPro; IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
DR   PANTHER; PTHR42737; PTHR42737; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   Pfam; PF02852; Pyr_redox_dim; 1.
DR   SUPFAM; SSF51905; SSF51905; 2.
DR   SUPFAM; SSF55424; SSF55424; 1.
DR   TIGRFAMs; TIGR01421; gluta_reduc_1; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; FAD; Flavoprotein; NADP; Oxidoreductase;
KW   Redox-active center; Reference proteome.
FT   CHAIN           <1..424
FT                   /note="Glutathione reductase"
FT                   /id="PRO_0000067957"
FT   ACT_SITE        413
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   DISULFID        32
FT                   /note="Interchain"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   424 AA;  46301 MW;  0714FF531F90BEBA CRC64;
     VNVGCVPKKV MWNTAVHSEF IHDHVDYGFQ NCKSKFNWHV IKEKRDAYVS RLNNIYQNNL
     TKSHIEVIHG YATFRDGPQP TAEVNGKKFT APHILIATGG VPTVPHENQI PGASLGITSD
     GFFQLEDLPS RSVIVGAGYI AVEIAGILSA LGSKTSLMIR HDKVLRSFDS LISSNCTEEL
     ENAGGVEVLT VKKFSQVKEV KKTSSGLELH VVTALPGRKP TVTTIPDVDC LLWAIGRDPN
     SKGLNLNKLG IQTDDKGHIL VDEFQNTNVK GVYAVGDVCG KALLTPVAIA AGRKLAHRLF
     EGKEDSRLDY DNIPTVVFSH PPIGTVGLTE DEAVHKYGKD NVKIYSTAFT PMYHAVTTRK
     TKCVMKMVCA NKEEKVVGIH MQGIGCDEML QGFAVAVKMG ATKADFDNRV AIHPTSSEEL
     VTLR