ID   GSIB_PECAS              Reviewed;         514 AA.
AC   Q6D3B0;
DT   06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Glutathione-binding protein GsiB {ECO:0000250|UniProtKB:P75797};
DE   Flags: Precursor;
GN   Name=gsiB {ECO:0000250|UniProtKB:P75797}; OrderedLocusNames=ECA2834;
OS   Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia
OS   carotovora subsp. atroseptica).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=218491;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=15263089; DOI=10.1073/pnas.0402424101;
RA   Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J.,
RA   Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K.,
RA   Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J.,
RA   Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Norbertczak H.,
RA   Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S.,
RA   Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.;
RT   "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora
RT   subsp. atroseptica and characterization of virulence factors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004).
CC   -!- FUNCTION: Part of the ABC transporter complex GsiABCD involved in
CC       glutathione import. Binds glutathione. {ECO:0000250|UniProtKB:P75797}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (GsiA),
CC       two transmembrane proteins (GsiC and GsiD) and a solute-binding protein
CC       (GsiB). {ECO:0000250|UniProtKB:P75797}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250|UniProtKB:P75797}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 5 family.
CC       {ECO:0000305}.
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DR   EMBL; BX950851; CAG75734.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q6D3B0; -.
DR   SMR; Q6D3B0; -.
DR   STRING; 218491.ECA2834; -.
DR   EnsemblBacteria; CAG75734; CAG75734; ECA2834.
DR   KEGG; eca:ECA2834; -.
DR   eggNOG; COG0747; Bacteria.
DR   HOGENOM; CLU_017028_7_3_6; -.
DR   OMA; KESPWVP; -.
DR   Proteomes; UP000007966; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:UniProt.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   InterPro; IPR030678; Peptide/Ni-bd.
DR   InterPro; IPR039424; SBP_5.
DR   InterPro; IPR000914; SBP_5_dom.
DR   PANTHER; PTHR30290; PTHR30290; 1.
DR   Pfam; PF00496; SBP_bac_5; 1.
DR   PIRSF; PIRSF002741; MppA; 1.
PE   3: Inferred from homology;
KW   Periplasm; Reference proteome; Signal; Transport.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..514
FT                   /note="Glutathione-binding protein GsiB"
FT                   /id="PRO_0000279972"
SQ   SEQUENCE   514 AA;  56724 MW;  374D93CB2B02FDF1 CRC64;
     MSVMTIQRRW LVAAGVTAAM VASPVWAAKD AVIAVGSTFT SLDPYDANDS LSQTVAKSFY
     QGLFGFDKDM KLINVLADSY DISSDGLTYT VKLHPGVKFH DGSAFNAAAV KVNLDRASNP
     DNRLKRYNLF KMIDKTEAVD DLTVKITLKT PFSAFVNNLA HPAAVMISPA ALTQYGKEIG
     FHPVGTGPYR FVAWNQTDFV KVEKFSGYWK AGLPKLDSIT WRPVVDNNTR AALLQTGEAQ
     FAYPIPFEQA KVLEKNDKLA LVASPSILHR YISMNVTQKP FDNPKVRQAL NYAINKDALI
     KVAFSGYATP AEGPLPNSID YSVKYHPWPY DPAKARELLK EAGYPNGFTT TLWSSHNHST
     AQKVLQFTQQ QLAQVGVKVQ VTAMDAGQRA AEVEGKGVKE TGVRLFYTGW SASTGEADWA
     LSPLFATASW PPAQFNTAFY SNPQVDTDLA NALKTTDRAE KQTLYKDAQD KIWADAPWIF
     LATERLVSAN SKKLTGFYVM PDTLFSFEDA DLTE