AMPP2_COCP7
ID AMPP2_COCP7 Reviewed; 487 AA.
AC C5PAH2;
DT 27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-SEP-2009, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Probable Xaa-Pro aminopeptidase CPC735_009100;
DE EC=3.4.11.9;
DE AltName: Full=Aminoacylproline aminopeptidase;
DE AltName: Full=Prolidase;
GN ORFNames=CPC735_009100;
OS Coccidioides posadasii (strain C735) (Valley fever fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Onygenales; Onygenaceae; Coccidioides.
OX NCBI_TaxID=222929;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C735;
RX PubMed=19717792; DOI=10.1101/gr.087551.108;
RA Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R.,
RA Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y.,
RA McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M.,
RA Barker B.M., Galgiani J.N., Orbach M.J., Kirkland T.N., Cole G.T.,
RA Henn M.R., Birren B.W., Taylor J.W.;
RT "Comparative genomic analyses of the human fungal pathogens Coccidioides
RT and their relatives.";
RL Genome Res. 19:1722-1731(2009).
CC -!- FUNCTION: Catalyzes the removal of a penultimate prolyl residue from
CC the N-termini of peptides. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Release of any N-terminal amino acid, including proline, that
CC is linked to proline, even from a dipeptide or tripeptide.;
CC EC=3.4.11.9;
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 2 manganese ions per subunit. {ECO:0000250};
CC -!- SIMILARITY: Belongs to the peptidase M24B family. {ECO:0000305}.
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DR EMBL; ACFW01000030; EER26734.1; -; Genomic_DNA.
DR RefSeq; XP_003068879.1; XM_003068833.1.
DR AlphaFoldDB; C5PAH2; -.
DR SMR; C5PAH2; -.
DR PRIDE; C5PAH2; -.
DR EnsemblFungi; EER26734; EER26734; CPC735_009100.
DR GeneID; 9694362; -.
DR KEGG; cpw:CPC735_009100; -.
DR VEuPathDB; FungiDB:CPC735_009100; -.
DR HOGENOM; CLU_017266_1_2_1; -.
DR Proteomes; UP000009084; Unassembled WGS sequence.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0070006; F:metalloaminopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.40.350.10; -; 1.
DR Gene3D; 3.90.230.10; -; 1.
DR InterPro; IPR007865; Aminopep_P_N.
DR InterPro; IPR029149; Creatin/AminoP/Spt16_NTD.
DR InterPro; IPR036005; Creatinase/aminopeptidase-like.
DR InterPro; IPR000994; Pept_M24.
DR InterPro; IPR001131; Peptidase_M24B_aminopep-P_CS.
DR Pfam; PF05195; AMP_N; 1.
DR Pfam; PF00557; Peptidase_M24; 1.
DR SMART; SM01011; AMP_N; 1.
DR SUPFAM; SSF53092; SSF53092; 1.
DR SUPFAM; SSF55920; SSF55920; 1.
DR PROSITE; PS00491; PROLINE_PEPTIDASE; 1.
PE 3: Inferred from homology;
KW Aminopeptidase; Hydrolase; Manganese; Metal-binding; Metalloprotease;
KW Protease.
FT CHAIN 1..487
FT /note="Probable Xaa-Pro aminopeptidase CPC735_009100"
FT /id="PRO_0000411832"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 275
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 286
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 286
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 421
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 460
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 460
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
SQ SEQUENCE 487 AA; 54980 MW; C26B4C0FBE5FAF02 CRC64;
MAGSNTLSSS EHGDDPRGHS YSIHIQTRGT TLDKYPAKQH ARNVARQLGL TDGLIYLMSQ
STRTLEDSDQ PQPFRQRRYF FYLSGVDEPD CHLTFDIKSD ILTLYVPHYD LRKAIWVGPT
LRPSEAMMRY DLNAAKTYDE LSKNIRTWAS KRMSPVIYIL HEGQKPNINA HFLAFNHEDL
LPAMDACREI KDEHEIDLIR RANEISASAH IEVLLGIRNM QNEAEIHGKF LDTCVSQGAR
NQSYEIIAAS GENAAILHYT KNNEPLDDRQ LVCLDAGAEW NCYASDVTRT FPRRPYWPSC
ESANIYSVVQ RMQEECINGL KEGVRYLDLH ILAHRIAIEE LLSLGILKGG STEEILQSGA
SLVFFPHGLG HHVGLEVHDV SPTPLMAFSL DKYKGLPLLS CRPPCTLSAP YLKAGMVVTV
EPGIYFSRPA LKDARRKPLS KYIDMDVVQK YIPVGGVRIE DDILVTRDGF ENLTKAPKGR
EMLKTIR
