GSID_ECOLI
ID GSID_ECOLI Reviewed; 303 AA.
AC P75799;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Glutathione transport system permease protein GsiD {ECO:0000305};
GN Name=gsiD {ECO:0000303|PubMed:16109926}; Synonyms=yliD;
GN OrderedLocusNames=b0832, JW0816;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION IN GLUTATHIONE TRANSPORT, AND SUBUNIT.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=16109926; DOI=10.1128/jb.187.17.5861-5867.2005;
RA Suzuki H., Koyanagi T., Izuka S., Onishi A., Kumagai H.;
RT "The yliA, -B, -C, and -D genes of Escherichia coli K-12 encode a novel
RT glutathione importer with an ATP-binding cassette.";
RL J. Bacteriol. 187:5861-5867(2005).
RN [5]
RP INTERACTION WITH GSIB.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=30515393; DOI=10.1155/2018/3429569;
RA Wang Z., Xia X., Zhang M., Fang J., Li Y., Zhang M.;
RT "Purification and characterization of glutathione binding protein GsiB from
RT Escherichia coli.";
RL Biomed. Res. Int. 2018:3429569-3429569(2018).
CC -!- FUNCTION: Part of the ABC transporter complex GsiABCD involved in
CC glutathione import. Probably responsible for the translocation of the
CC substrate across the membrane. {ECO:0000305|PubMed:16109926}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (GsiA),
CC two transmembrane proteins (GsiC and GsiD) and a solute-binding protein
CC (GsiB) (Probable). In the presence of glutathione, interacts with the
CC glutathione-binding protein GsiB (PubMed:30515393).
CC {ECO:0000269|PubMed:30515393, ECO:0000305|PubMed:16109926}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. {ECO:0000305}.
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DR EMBL; U00096; AAC73919.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35527.1; -; Genomic_DNA.
DR PIR; H64820; H64820.
DR RefSeq; NP_415353.1; NC_000913.3.
DR RefSeq; WP_001236051.1; NZ_SSZK01000002.1.
DR AlphaFoldDB; P75799; -.
DR BioGRID; 4259984; 35.
DR ComplexPortal; CPX-4325; Glutathione ABC transporter complex.
DR IntAct; P75799; 1.
DR STRING; 511145.b0832; -.
DR TCDB; 3.A.1.5.11; the atp-binding cassette (abc) superfamily.
DR PaxDb; P75799; -.
DR PRIDE; P75799; -.
DR EnsemblBacteria; AAC73919; AAC73919; b0832.
DR EnsemblBacteria; BAA35527; BAA35527; BAA35527.
DR GeneID; 945461; -.
DR KEGG; ecj:JW0816; -.
DR KEGG; eco:b0832; -.
DR PATRIC; fig|1411691.4.peg.1446; -.
DR EchoBASE; EB3248; -.
DR eggNOG; COG1173; Bacteria.
DR HOGENOM; CLU_028518_1_1_6; -.
DR InParanoid; P75799; -.
DR OMA; RAWWVVS; -.
DR PhylomeDB; P75799; -.
DR BioCyc; EcoCyc:YLID-MON; -.
DR BioCyc; MetaCyc:YLID-MON; -.
DR BRENDA; 7.4.2.10; 2026.
DR PRO; PR:P75799; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0071916; F:dipeptide transmembrane transporter activity; IBA:GO_Central.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR InterPro; IPR025966; OppC_N.
DR Pfam; PF00528; BPD_transp_1; 1.
DR Pfam; PF12911; OppC_N; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..303
FT /note="Glutathione transport system permease protein GsiD"
FT /id="PRO_0000060262"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 101..290
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ SEQUENCE 303 AA; 33238 MW; 94586DD1037F9D5E CRC64;
MRLFNWRRQA VLNAMPLVKP DQVRTPWHEF WRRFRRQHMA MTAALFVILL IVVAIFARWI
APYDAENYFD YDNLNNGPSL QHWFGVDSLG RDIFSRVLVG AQISLAAGVF AVFIGAAIGT
LLGLLAGYYE GWWDRLIMRI CDVLFAFPGI LLAIAVVAVL GSGIANVIIA VAIFSIPAFA
RLVRGNTLVL KQQTFIESAR SIGASDMTVL LRHILPGTVS SIVVFFTMRI GTSIISAASL
SFLGLGAQPP TPEWGAMLNE ARADMVIAPH VAVFPALAIF LTVLAFNLLG DGLRDALDPK
IKG
