ID   GSK4_ORYSJ              Reviewed;         403 AA.
AC   Q5Z7J0; O04401;
DT   15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 140.
DE   RecName: Full=Shaggy-related protein kinase GSK4 {ECO:0000305};
DE            EC=2.7.11.1 {ECO:0000305};
DE   AltName: Full=Glycogen synthase kinase3-like protein 4 {ECO:0000305};
DE            Short=OsGSK4 {ECO:0000305};
DE   AltName: Full=Shaggy-related protein kinase etha homolog {ECO:0000305};
DE            Short=OsKETHA {ECO:0000303|PubMed:22570626};
DE            Short=SKETHA {ECO:0000303|PubMed:22570626};
GN   Name=GSK4 {ECO:0000305};
GN   OrderedLocusNames=Os06g0547900 {ECO:0000312|EMBL:BAF19738.1},
GN   LOC_Os06g35530 {ECO:0000305};
GN   ORFNames=OsJ_21630 {ECO:0000312|EMBL:EAZ37291.1},
GN   OSJNBa0016D02.31 {ECO:0000312|EMBL:BAD54124.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Nipponbare; TISSUE=Root;
RA   Dornelas M.C., Kreis M.;
RT   "Oryza sativa mRNA for shaggy-like kinase etha.";
RL   Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [7]
RP   INTERACTION WITH LIC.
RX   PubMed=22570626; DOI=10.1371/journal.pgen.1002686;
RA   Zhang C., Xu Y., Guo S., Zhu J., Huan Q., Liu H., Wang L., Luo G., Wang X.,
RA   Chong K.;
RT   "Dynamics of brassinosteroid response modulated by negative regulator LIC
RT   in rice.";
RL   PLoS Genet. 8:E1002686-E1002686(2012).
CC   -!- FUNCTION: Probable serine-threonine kinase that may regulate
CC       brassinosteroid signaling. {ECO:0000250|UniProtKB:Q60EZ2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC         Evidence={ECO:0000305};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.1; Evidence={ECO:0000305};
CC   -!- SUBUNIT: Interacts with LIC. {ECO:0000269|PubMed:22570626}.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr
CC       protein kinase family. GSK-3 subfamily. {ECO:0000305}.
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DR   EMBL; Y13437; CAA73848.1; -; mRNA.
DR   EMBL; AP004731; BAD54124.1; -; Genomic_DNA.
DR   EMBL; AP008212; BAF19738.1; -; Genomic_DNA.
DR   EMBL; AP014962; BAS98159.1; -; Genomic_DNA.
DR   EMBL; CM000143; EAZ37291.1; -; Genomic_DNA.
DR   EMBL; AK100950; BAG94846.1; -; mRNA.
DR   PIR; T03777; T03777.
DR   RefSeq; XP_015644139.1; XM_015788653.1.
DR   AlphaFoldDB; Q5Z7J0; -.
DR   SMR; Q5Z7J0; -.
DR   STRING; 4530.OS06T0547900-02; -.
DR   iPTMnet; Q5Z7J0; -.
DR   PaxDb; Q5Z7J0; -.
DR   PRIDE; Q5Z7J0; -.
DR   EnsemblPlants; Os06t0547900-01; Os06t0547900-01; Os06g0547900.
DR   EnsemblPlants; Os06t0547900-02; Os06t0547900-02; Os06g0547900.
DR   GeneID; 4341251; -.
DR   Gramene; Os06t0547900-01; Os06t0547900-01; Os06g0547900.
DR   Gramene; Os06t0547900-02; Os06t0547900-02; Os06g0547900.
DR   KEGG; osa:4341251; -.
DR   eggNOG; KOG0658; Eukaryota.
DR   HOGENOM; CLU_000288_181_20_1; -.
DR   InParanoid; Q5Z7J0; -.
DR   OMA; VRNICHR; -.
DR   OrthoDB; 990896at2759; -.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000007752; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR   GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR   GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR   CDD; cd14137; STKc_GSK3; 1.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR017441; Protein_kinase_ATP_BS.
DR   InterPro; IPR008271; Ser/Thr_kinase_AS.
DR   InterPro; IPR039192; STKc_GSK3.
DR   Pfam; PF00069; Pkinase; 1.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Brassinosteroid signaling pathway; Kinase; Nucleotide-binding;
KW   Reference proteome; Serine/threonine-protein kinase; Transferase.
FT   CHAIN           1..403
FT                   /note="Shaggy-related protein kinase GSK4"
FT                   /id="PRO_0000439009"
FT   DOMAIN          71..355
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   ACT_SITE        196
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         77..85
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         100
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   CONFLICT        213
FT                   /note="V -> L (in Ref. 1; CAA73848)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   403 AA;  45307 MW;  1C17C21B081E814A CRC64;
     MAAMPGGPDL AGAGGAVAVA VDAMQVDDPP RASAEEKHGP TIMGGNDPVT GHIISTTIGG
     KNDEPKRTIS YMAERVVGTG SFGVVFQAKC LETGETVAIK KVLQDKRYKN RELQIMRSMD
     HCNVISLKHC FFSTTSRDEL FLNLVMEFVP ESLYRVLKHY KDMKQRMPLI YVKLYMYQIF
     RGLAYIHTVP GVCHRDIKPQ NILVDPLTHQ VKVCDFGSAK MLIKGEANIS YICSRYYRAP
     ELIFGATEYT TSIDIWSAGC VLAELLLGQP LFPGESAVDQ LVEIIKVLGT PTREEIRCMN
     PNYTEFKFPQ IKACPWHKIF HKRMPPEAID LVSRLLQYSP NLRCTALEAC AHSFFDELRE
     PHAKLPNGRP FPPLFNFKQE LANTHPELVS RLLPEHAQRH SGF